A5101917706- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- T-cell and Retrovirus Studies
- Whipple's Disease and Interleukins
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Fungal Infections and Studies
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Infective Endocarditis Diagnosis and Management
- COVID-19 epidemiological studies
- Adhesion, Friction, and Surface Interactions
- Long-Term Effects of COVID-19
- 3D IC and TSV technologies
- Plant and Fungal Interactions Research
- RNA regulation and disease
- Viral Infections and Outbreaks Research
National Institute for Viral Disease Control and Prevention
2019-2025
Center for Disease Control
2021-2023
Anhui University of Science and Technology
2023
Nanchang Center for Disease Control and Prevention
2023
Chinese Center For Disease Control and Prevention
2021
In recent years, coxsackievirus A6 (CVA6) has surpassed enterovirus A71 to become the main pathogen causing severe Hand, Foot, and Mouth disease (HFMD) in China with a substantial burden. However, there is currently no commercial CVA6 vaccine. The D3a genotype of predominant China. this study, virus-like particles (VLPs) mRNA vaccines based on sub-genotype were successfully developed. immunogenicity protective effects VLP combined Al(OH)3 CpG adjuvant indicated that VLP-induced neutralizing...
Abstract Hand, foot, and mouth disease (HFMD), which is a frequently reported concerning worldwide, severe burden on societies globally, especially in the countries of East Southeast Asia. Coxsackievirus A16 (CV-A16) one most important causes HFMD threat to human health, children under 5 years age. To investigate epidemiological characteristics, spread dynamics, recombinant forms (RFs), other features CV-A16, we leveraged continuous surveillance data CV-A16-related cases collected over an...
After 56 days without coronavirus disease 2019 (COVID-19) cases, reemergent cases were reported in Beijing, China on June 11, 2020. Here, we report the genetic characteristics of severe acute respiratory syndrome 2 (SARS-CoV-2) sequenced from clinical specimens 4 human and environmental samples. The nucleotide similarity among six SARS-CoV-2 genomes ranged 99.98% to 99.99%. Compared with reference strain (GenBank No. NC_045512), all genome sequences shared same substitutions at nt241 (C →...
ABSTRACT China experienced another outbreak of acute hemorrhagic conjunctivitis (AHC) in 2023, with a total 195 297 recorded cases. This marks the third nationwide nearly two decades, following previous outbreaks 2007 and 2010. Descriptive epidemiological analysis shows that number cases 2023 peaked September, similar to last outbreaks. The age groups highest incidence rate three 2007, 2010, are 15–20, 10–15, 10–15 years old, respectively. Students main occupational group Hainan, Guangxi,...
Multiple subtypes of avian influenza virus (AIV), including H5N1, H5N6, and H5N8 viruses, are currently co-circulating in wild birds poultry causing sporadic human infections. Vaccine development is essential for pandemic preparedness. In this study, we constructed a candidate vaccine (CVV) using reverse genetics (RG) based on the sequence first human-infected subtype AIV, A/Astrakhan/3212/2020 (H5N8). We evaluated immunogenicity rH5N8/PR8 strain combination with Alum, ISA51, MF59 adjuvants,...
ABSTRACT Coxsackievirus A10 (CVA10) has been reported frequently in many infectious diseases and cases associated with hand, foot, mouth disease (HFMD) emerging increasingly recent years. Based on the National HFMD System Surveillance, 180 CVA10 strains were isolated from mainland of China between 2008 2023. These analyzed alongside 103 representative full VP1 sequences obtained GenBank, a focus global‐scale phylogenetic analysis spatiotemporal dynamics CVA10. Eight genotypes (A–H) defined,...
Abstract Hand, foot, and mouth disease (HFMD) is a common global epidemic. From 2008 onwards, many HFMD outbreaks caused by coxsackievirus A6 (CV-A6) have been reported worldwide. Since 2013, with dramatically increasing number of CV-A6-related cases, CV-A6 has become the predominant pathogen in mainland China. Phylogenetic analysis based on VP1 capsid gene revealed that subtype D3 dominated outbreaks. Here, we performed large-scale (near) full-length genetic Chinese variants, including 158...
G9P[8] became the predominant rotavirus A (RVA) genotype in China 2012. To evaluate its genetic composition at whole-genome level, 115 RVA strains isolated from children under 5 years old were sequenced and characterized. All 13 2016 2017 an additional 54 2018 genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1- E1 -H1. The other 48 all E2 -H1, with NSP4 gene characterized a DS-1-like genotype. time of most recent common ancestor (tMRCA) evolution rates VP7, VP4, (E1 E2) genes these estimated by...
Hand, foot, and mouth disease (HFMD) caused by a group of enteroviruses is global public health problem. In recent years, coxsackievirus A6 (CVA6) has emerged as an important HFMD agent. Previous studies have shown that mutations glycine 64 in RNA-dependent RNA polymerase (3D polymerase), which central to viral replication, cause phenotypic changes such ribavirin resistance, increased replication fidelity, virulence attenuation poliovirus enterovirus A71. this study, we constructed CVA6...
Echovirus 30 (E30) infection causes a wide spectrum of diseases with mild symptoms, such as hand, foot, and mouth disease (HFMD), acute flaccid paralysis, aseptic meningitis other diseases, especially one the most common pathogens causing outbreaks. We established novel mouse model E30 by inoculating neonatal mice clinical isolates observed pathological changes induced E30.
In recent years, the prevalence of hand, foot, and mouth disease (HFMD) caused by enteroviruses other than enterovirus A71 (EV-A71) coxsackievirus A16 (CVA16) has gradually increased. The throat swab specimens 2701 HFMD cases were tested, VP1 regions CVA10 RNA amplified using RT-PCR, phylogenetic analysis was performed. Children aged 1–5 years accounted for majority (81.65%) boys more girls. positivity rates EV-A71, CVA16, EVs 15.22% (219/1439), 28.77% (414/1439), 56.01% (806/1439),...
Nineteen CVA9 isolates were obtained between 2010 and 2019 from six provinces of mainland China, using the HFMD surveillance network established in China. Nucleotide sequencing revealed that full-length VP1 19 was 906 bases encoding 302 amino acids. The combination thresholds phylogenetic tree nucleotide divergence different genotypes within same serotype led to a value 15–25%, enabled worldwide be categorized into ten genotypes: A–J. showed prototype strain included genotype A, B, C, D, E,...
Enterovirus A71 (EV-A71) is one of the main pathogens causing hand, foot, and mouth disease (HFMD) outbreaks in Asian children under 5 years age. In severe cases, it can cause neurological complications be life-threatening. this study, 200 newly sequenced EV-A71 whole-genome sequences were combined with 772 from GenBank for large-scale analysis to investigate global epidemiology, phylogeny, Bayesian phylodynamic characteristics. Based on phylogenetic 3Dpol region, six new evolutionary...
The aim of this study was to determine the global genetic diversity and transmission dynamics coxsackievirus B4 (CVB4) propose future directions for disease surveillance. Next-generation sequencing performed obtain complete genome sequence CVB4, CVB4 worldwide were analyzed using bioinformatics methods such as phylogenetic analysis, evolutionary dynamics, phylogeographic analysis. Forty genomes identified from asymptomatic infected individuals hand, foot, mouth (HFMD) patients. Frequent...
As the proportion of non-enterovirus 71 and non-coxsackievirus A16 which composition in hand, foot, mouth pathogenic spectrum gradually increases worldwide, attention paid to other enteroviruses has increased. a member species enterovirus A, coxsackievirus A14 (CVA14) been epidemic around world until now since it isolated. However, studies on CVA14 are poor effective population size, evolutionary dynamics, recombination patterns not well understood. In this study, 15 strains were isolated...
Abstract Enterovirus B83 (EV-B83) is a new member of the enterovirus B group. Currently, there are only two full-length genomic sequences EV-B83 in GenBank database and few VP1 region sequences. The aetiology epidemiology unclear. 24 stool specimens were collected from twelve AFP patients 298 healthy children support polio eradication activities Tibet 1999. Two polioviruses (isolated by L20B cell) one non-polio RD isolated nine 90 enteroviruses health children. Through molecular typing, we...
EV-A120 is a recently identified serotype of the enterovirus A species. Only one full-length genomic sequence currently available in GenBank, and very few studies have been conducted on globally. Thus, additional information research are needed to explore its genetic characteristics, phylogeny, relationship with enteroviral disease. In this study, we report phylogenetic characteristics strain (Q0082/XZ/CHN/2000) from Tibet, China. The amino acid similarity nucleotide prototype were 96.3%...
Abstract Enterovirus C99 (EV‐C99) is a newly identified EV serotype within the species C. Few studies on EV‐C99 have been conducted globally. More information and research are needed to assess its genetic characteristics, phylogenetic relationships, associations with enteroviral diseases. Here, characteristics of 11 Chinese strains reported. The full‐length genomic sequences these show 79.4–80.5% nucleotide identity 91.7–94.3% amino acid (aa) prototype EV‐C99. A maximum likelihood tree...
Abstract Echovirus 25 (E25), a member of the Enterovirus B (EV-B) species, can cause aseptic meningitis (AM), viral (VM), and acute flaccid paralysis (AFP). However, systematic studies on molecular epidemiology E25, especially those concerning its evolution recombination, are lacking. In this study, 18 strains isolated from seven provinces China between 2009 2018, were collected based Chinese hand, foot, mouth disease (HFMD) surveillance network, 95 sequences downloaded GenBank also...
Abstract Enterovirus C116 (EV‐C116) is a new member of the enterovirus C group which closely associated with several infectious diseases. Although sporadic studies have detected EV‐C116 in clinical samples worldwide, there currently limited information available. In this study, two EV‐C‐positive fecal specimens were apparently healthy children, harbored low abundance, through meta‐transcriptome sequencing. Based on prototypes EV‐Cs, lineages observed. Lineage 1 included many types that could...
Novel posa-like viral genomes were first identified in swine fecal samples using metagenomics and designated as unclassified viruses the order Picornavirales. In present study, nine husavirus strains China. Their share 94.1–99.9% similarity, alignment of these 697 nucleotide polymorphism sites across their full-length genomes. These directly clustered with Husavirus 1 lineage, genomic arrangement showed similar characteristics. have undergone a complex evolutionary process, wide geographic...