A5012045710- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Schizophrenia research and treatment
- Mental Health Treatment and Access
- Chemical Synthesis and Analysis
- Neural dynamics and brain function
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- Connective tissue disorders research
- Pluripotent Stem Cells Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Peptidase Inhibition and Analysis
- Neurogenesis and neuroplasticity mechanisms
- FOXO transcription factor regulation
- Autophagy in Disease and Therapy
- Fibroblast Growth Factor Research
- CRISPR and Genetic Engineering
- Synthesis of Indole Derivatives
- Genomic variations and chromosomal abnormalities
- Marine animal studies overview
- Genetics and Neurodevelopmental Disorders
- Suicide and Self-Harm Studies
- Protein Degradation and Inhibitors
- Hereditary Neurological Disorders
University of Pennsylvania
2020-2025
California University of Pennsylvania
2021-2024
Albert Einstein College of Medicine
2010-2017
Yeshiva University
2013-2015
Institut thématique Génétique, génomique et bioinformatique
2014
McGill University
2009
Orange County School
1999
Mutations in Kinesin proteins (Kifs) are linked to various neurological diseases, but the specific and redundant functions of vertebrate Kifs incompletely understood. For example, Kif5A, not other Kinesin-1 heavy-chain family members, is implicated Charcot-Marie-Tooth disease (CMT) Hereditary Spastic Paraplegia (HSP), mechanism its involvement progressive axonal degeneration characteristic these diseases well We report that zebrafish kif5Aa mutants exhibit hyperexcitability, peripheral...
Live imaging of transcription and RNA dynamics has been successful in cultured cells tissues vertebrates but is challenging to accomplish vivo. The zebrafish offers important advantages study these processes--optical transparency during embryogenesis, genetic tractability rapid development. Therefore, an intact vertebrate organism, we have adapted the MS2 RNA-labeling system zebrafish. By using this binary coexpress a fluorescent bacteriophage coat protein (MCP) interest tagged with multiple...
In animals, specification of the primordial germ cells (PGCs), stem germline, is required to transmit genetic information from one generation next. Bucky ball (Buc) essential for plasm (GP) assembly in oocytes and its overexpression results excess PGCs zebrafish embryos. However, mechanistic basis response Buc overexpression, whether endogenous functions during embryogenesis are unknown. Here we show that Buc, like GP overexpressed Buc-GFP, accumulates at embryonic cleavage furrows....
Prolyl oligopeptidases cleave peptides on the carboxy side of internal proline residues and their inhibition has potential in treatment human brain disorders. Using our docking program fitted, we have designed a series constrained covalent inhibitors, built from bicyclic scaffolds, to study optimal shape required for these small molecules. These structures bear nitrile functional groups that predicted covalently bind catalytic serine enzyme. Synthesis biological assays using brain-derived...
Behavioral thresholds define the lowest stimulus intensities sufficient to elicit a behavioral response. Establishment of baseline during development is critical for proper responses throughout animal’s life. Despite relevance such innate thresholds, molecular mechanisms establishing are not well understood. The acoustic startle response conserved behavior whose threshold established yet subsequently acutely regulated. We have previously identified zebrafish mutant line ( escapist ) that...
ABSTRACT Aim Connection Learning Healthcare System, one of the eight hubs National Institute Mental Health funded Early Psychosis Intervention Network, supports uniform data collection, analysis, feedback and infrastructure development to promote a culture continuous quality improvement across 25 Coordinated Specialty Care programs serving young people experiencing first episode psychosis their families in Maryland Pennsylvania. This report describes hub its constituent programs, approach...
During skeletal morphogenesis diverse mechanisms are used to support bone formation. This can be seen in the bones that require a cartilage template for their development. In mammals is removed, but zebrafish persists and mineralizes around scaffold. Remodeling of unmineralized occurs via planar cell polarity (PCP) mediated rearrangements contribute lengthening elements; however, maintain chondrocyte supports perichondral ossification remain unclear. We report double mutants disrupting two...
Abstract Microdeletion of a 3Mb region encompassing 45 protein-coding genes at chromosome 22q11.2 (22q11.2DS) predisposes individuals to multiple neurodevelopmental disorders and is one the greatest genetic risk factors for schizophrenia. Defective mitochondrial function has been hypothesized contribute 22q11.2DS pathogenesis; however, which six phenotypes their underlying mechanisms remain unresolved. To systematically test functional roles in neurodevelopment behavior, we generated mutants...
ABSTRACT Individuals with monoallelic pathogenic variants in the histone lysine methyltransferase DOT1L display global developmental delay and varying congenital anomalies. However, impact of loss remains unclear. Here, we present a largely female cohort 11 individuals delays dysmorphic facial features. We found that include missense clustered catalytic domain, frameshift, stop-gain variants. demonstrate specific cause activity therefore sought to define effects decreased function. Using...
Summary Microdeletion of a 3Mbp region encompassing 45 protein-coding genes at chromosome 22q11.2 (22q11.2DS) predisposes to multiple neurodevelopmental disorders and is one the greatest genetic risk factors for schizophrenia. Defective mitochondrial function has been hypothesized contribute 22q11.2DS pathogenesis; however, which six phenotypes their underlying mechanisms remain unresolved. To systematically test functional roles in neurodevelopment behavior, we generated mutants each 37...
A series of 23 neutral, anionic, and zwitterionic surfactants were tested at a concentration 0.1% wt/vol for their influence on attachment Mycobacterium sp. to cellulose acetate (CA) polyamide (PA) reverse osmosis (RO) membranes. Four cell bioassays used: (1) semiconcurrent addition surfactant bacteria RO coupons (standard assay); (2) pretreatment membranes (membrane (3) treatment adsorbed cells (detachment (4) mycobacteria (cell assay). Seventeen inhibited PA membranes, whereas 15 CA in...
Abstract An organocatalytic oxa‐Mannich/aldol reaction is developed to give chromenes in high yields without the need of a co‐catalyst.
Abstract Behavioral thresholds define the lowest stimulus intensities sufficient to elicit a behavioral response. Establishment of baseline during development is critical for proper responses throughout animal’s life. Despite relevance such innate thresholds, molecular mechanisms establishing are not well understood. The acoustic startle response conserved behavior whose threshold established yet subsequently acutely regulated. We have previously identified zebrafish mutant line ( escapist )...
VITAL ROLES OF KINESINS IN DEVELOPMENT AND DISEASE ZEBRAFISH Philip D. Campbell. Mentor: Dr. Florence L. Marlow HOW THE BRAIN HANDLES SENSORY UNCERTAINTY Fanny Cazettes. Jose Pena OPTICAL TOOLS TO STUDY ISOFORM-SPECIFIC SMALL GTPASES IMMUNE CELLS Veronika Miskolci. Dianne Cox & Louis Hodgson NEUTROPHIL AGING IS REGULATED BY MICROBIOME Dachuan Zhang. Paul S. Frenette