A5085188266- HIV/AIDS Research and Interventions
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- SARS-CoV-2 detection and testing
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 and COVID-19 Research
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 epidemiological studies
- Clinical Laboratory Practices and Quality Control
- Blood groups and transfusion
- COVID-19 diagnosis using AI
- Syphilis Diagnosis and Treatment
- Data-Driven Disease Surveillance
- Tuberculosis Research and Epidemiology
- HIV, Drug Use, Sexual Risk
- Mobile Health and mHealth Applications
- Immunodeficiency and Autoimmune Disorders
- Biosensors and Analytical Detection
- Electronic Health Records Systems
- Cytomegalovirus and herpesvirus research
- Global Maternal and Child Health
- Respiratory viral infections research
- Child and Adolescent Health
- Ethics in Clinical Research
- Genital Health and Disease
Instituto Nacional de Saúde
2011-2024
Fundação Ariel Glaser Contra o SIDA Pediátrico
2023
Ludwig-Maximilians-Universität München
2020-2023
Catholic University of Mozambique
2013
Objective: To evaluate the accuracy of a point-of-care (POC) nucleic acid–based test (NAT) for early infant HIV diagnosis (EID) in primary health clinics Mozambique. Methods: POC and laboratory NAT EID tests were conducted on matched blood samples collected from 827 HIV-exposed infants younger than 18 months who enrolled consecutively at 4 periurban central hospital Maputo. Lancet heel draw by nurses was tested site HIV-1/-2 RNA Alere device also used to create dried spots later testing...
To evaluate the accuracy of point-of-care tests (POCTs) for CD4 cell, clinical chemistry and hemoglobin in primary healthcare clinics Mozambique.POCT laboratory-based assays were conducted on adult HIV-positive patients enrolled consecutively at Mozambique. Patients tested on-site with POCT (Pima), (Reflotron) (HemoCue) devices using finger prick blood. Results obtained paired blood samples used agreement analysis (bias limits agreement). Repeatability was also performed cell...
Background Clinical laboratory reference values from North American and European populations are currently used in most Africans countries due to the absence of locally derived ranges, despite previous studies reporting significant differences between populations. Our aim was define ranges for both genders 18 24 year-old Mozambicans preparation clinical vaccine trials. Methods A cross-sectional study including 257 volunteers (102 males 155 females) years performedat a youth clinic Maputo,...
Viral load testing is the WHO-recommended monitoring assay for patients on HIV antiretroviral therapy (ART). Point-of-care (POC) assays may help improve access to viral in resource-limited settings. We compared performance of Alere Q NAT POC technology (Alere Technologies, Jena, Germany), measuring total RNA using finger prick capillary whole-blood samples collected a periurban health center, with that laboratory-based plasma test (Roche Cobas Ampliprep/Cobas TaqMan v2) conducted matched...
Background Prevalence of HIV in Mozambique among individuals aged 15–49 years is 11.5%. The prevalence higher women than men across the country, peaking at ages 25–29 and 35–39 years, respectively. In this study, we aimed determining incidence HIV, Hepatitis B (HBV), syphilis youths. We also characterized a cohort youths for future participation phase I/II vaccine trials. Methods study was conducted youth clinic Maputo Central Hospital from August 2009 to October 2011. Youths both genders...
Introduction Point-of-care (POC) CD4 testing can improve access to treatment by enabling decentralization and reducing patient loss-to-follow-up. As new POC technologies become available, their performance should be assessed before widespread deployment. This study reports the findings of five independent evaluations PointCare NOW system. Materials/Methods Evaluations were conducted in Southern Africa (Mozambique, South Africa) North America (Canada, USA). 492 blood samples (55 from...
BackgroundDespite antiretroviral treatment (ART), immune dysfunction persists in children with perinatal HIV infection (HEI). Here we investigated the impact of status on maternal antibody (Ab) passage, long-term vaccine induced immunity and B-cell maturation.Methods46 Exposed Uninfected (HEU), 43 HEI, 15 unexposed uninfected (HUU) infants were vaccinated 3 doses DTaP-HepB-Hib-PCV10-OP at 2, 3, 4 months Matola Provincial Hospital, Maputo, Mozambique. Tetanus toxoid specific (TT) IgG, Ab...
The expansion of HIV antiretroviral therapy into decentralized rural settings will increasingly require simple point-of-care (POC) diagnostic tests that can be used without laboratory infrastructure and technical skills. New POC test devices are becoming available but decisions around which technologies to deploy may biased systematic assessment their suitability for healthcare settings. To address this, we developed a standardized, quantitative scorecard tool objectively evaluate the...
Introduction Anemia, syphilis and HIV are high burden diseases among pregnant women in sub-Saharan Africa. A quasi-experimental study was conducted four health facilities Southern Mozambique to evaluate the effect of point-of-care technologies for hemoglobin quantification, testing CD4+ T-cell enumeration performed within maternal child services on treatment coverage, assessing acceptability by workers. Methods Demographic data attending first antenatal care were extracted from existing...
Background Globally, nearly 22 million HIV-infected patients are currently accessing antiretroviral treatment; however, almost one people living with HIV died of AIDS-related illnesses in 2018. Advanced disease remains a significant issue to curb HIV-related mortality. Methods We analyzed 864,389 CD4 testing records collected by 1,016 Alere Pima Analyzers implemented at variety facilities, including peripheral between January 2012 and December 2016 across four countries sub-Saharan Africa....
Background. Voluntary medical male circumcision (VMMC) is a priority HIV preventive intervention. To facilitate scale- up, the World Health Organization actively seeking techniques that are quicker, easier, and safer than open surgical methods. Objective. compare conventional with suturing minimally invasive technique using Gomco clamp plus tissue adhesive. Methods. We conducted non-blinded randomised controlled trial comprising 200 volunteers >18 years of age, seen at outpatient...
(1) Background: Laboratory-based molecular assays are the gold standard to detect SARS-CoV-2. In resource-limited settings, implementation of these has been hampered by operational challenges and long turnaround times. Rapid antigen detection tests an attractive alternative. Our aim is evaluate clinical performance two SARS-CoV-2 rapid during a high transmission period. (2) Methods: A total 1277 patients seeking diagnosis were enrolled at four health facilities. Nasopharyngeal swabs for real...
Background: In resource-poor countries, antigen-based rapid tests (Ag-RDTs) performed at primary healthcare and community settings improved access to SARS-CoV-2 diagnostics. However, the technical skills biosafety requirements inherent nasopharyngeal oropharyngeal (OP) specimens limit scale-up of testing. The collection nasal-swabs is programmatically viable, but its performance has not been evaluated in settings. Methods: We first SteriPack self-collected nasal swabs for detection by...
ABSTRACT Dried blood spot (DBS) may overestimate the viral RNA, mainly in patients with low load (VL), due to proviral DNA and intracellular RNA. The Burnett HemaSpot provide integrated solutions for collection, separation, drying of plasma from whole blood. This study aims evaluate performance both devices compared identify antiretroviral therapy (ART) failure. were separately evaluated a cross-sectional design. Patients on ART included studies (Burnett: 611, October 2019 January 2020)...
Plasma is considered the gold standard for HIV viral load (VL) testing, however its use challenging due to need phlebotomy and centrifugation services, as well cold chain transporting laboratories testing. The of Dried Blood Spot (DBS) specimen has allowed a rapid expansion antiretroviral therapy (ART) monitoring in remote areas many African countries, however, VL DBS may overestimate copies RNA result at clinically relevant range 1000 copies/ml, proviral DNA intracellular RNA....
Abstract Background The extent of population exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was uncertain in many African countries during the onset pandemic. Methods We conducted a cross-sectional study and randomly selected surveyed general occupational groups from 6 July 24 August 2020, 3 cities Mozambique. Anti–SARS-CoV-2–specific immunoglobulin M (IgM) G (IgG) antibodies were measured using point-of-care rapid test. prevalence weighted for (by age, sex, city)...
Introduction: Point-of-care (POC) CD4 testing can improve access to treatment by enabling decentralization and reducing patient loss-to-follow-up.As new POC technologies become available, their performance should be assessed before widespread deployment.This study reports the findings of five independent evaluations PointCare NOW system.Materials/Methods: Evaluations were conducted in Southern Africa (Mozambique, South Africa) North America (Canada, USA).492 blood samples (55 from...
Early diagnosis of SARS-CoV-2 is fundamental to reduce the risk community transmission and mortality, as well public sector expenditures. Three years after onset pandemic, there are still gaps on what known regarding costs cost drivers for major diagnostic testing strategies in low- middle-income countries (LMICs). This study aimed estimate symptomatic suspected patients by reverse transcription polymerase chain reaction (RT-PCR) antigen rapid tests (Ag-RDT) Mozambique. We conducted a...
No abstract available.
Our study aimed to evaluate the stability of human immunodeficiency virus 1 (HIV-1) RNA on cobas plasma separation card (PSC) specimens for viral load (VL) testing after being exposed varied temperatures and storage times.For this purpose, venous PSC were collected stored at 25ºC 42ºC a period up 28 days. Plasma VL baseline was used as reference, against which compared different time points.From 30 patients included in study, 600 fresh obtained. fewer than 1,000 copies/mL 16 patients, 99.4%...
Since 2010, point-of-care (POC) CD4 testing platforms have been introduced in both urban and rural settings to expand access by bringing diagnostic services closer patients. We conducted an analysis of routinely collected data determine the invalid result rates associated with POC testing.We analyzed 981,152 records from Alere Pima Analyzers between January 2011 December 2016 across five countries sub-Saharan Africa. Routinely programmatic were used rate test results per month, facility...
Conventional diagnostic systems struggled to meet the fluctuating demand for testing across different waves of coronavirus disease 2019 (COVID-19) pandemic. This study aimed assess feasibility and effectiveness walkthrough (WT) approach in extending access COVID-19 high-risk populations traditionally underrepresented at health facilities (HFs) observe its impact on demand. An interventional was implemented markets (WT markets) ports ports) Maputo City Province, Mozambique. Demographic,...