A5100335849- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Mast cells and histamine
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- COVID-19 Clinical Research Studies
- Polyamine Metabolism and Applications
- Cancer-related molecular mechanisms research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Asthma and respiratory diseases
- RNA Interference and Gene Delivery
- Urticaria and Related Conditions
- Immune Response and Inflammation
- melanin and skin pigmentation
- Lymphatic System and Diseases
- Effects of Vibration on Health
- COVID-19 and healthcare impacts
- NF-κB Signaling Pathways
- Lipoproteins and Cardiovascular Health
- interferon and immune responses
- Diagnosis and Treatment of Venous Diseases
- Long-Term Effects of COVID-19
- Vitamin C and Antioxidants Research
Shanghai Jiao Tong University
2006-2024
Ruijin Hospital
2023
Shanghai Institute of Hematology
2023
Northeast Agricultural University
2022
Renji Hospital
2017-2021
Cleveland Clinic
2019
Hangzhou Medical College
2017
Shanghai University of Traditional Chinese Medicine
2016
Jilin University
2014
Primary Children's Hospital
2013
Although mature dendritic cells (DCs) are potent initiators of adaptive immune response, immature steady-state DCs contribute to tolerance. In this study, we show that ex vivo splenic capable inducing conversion naïve CD4 + T Foxp3 regulatory (aTreg) in the presence TGF-β. particular, when compared with CD8α − DCs, DC subset were superior higher frequencies conversion. This was not attributable difference basal level costimulation, because deficiency CD40 or CD80/86 signaling did diminish...
The success of clinically relevant immunotherapies requires reversing tumor-induced immunosuppression. Here we demonstrated that linear polyethylenimine-based (PEI-based) nanoparticles encapsulating siRNA were preferentially and avidly engulfed by regulatory DCs expressing CD11c programmed cell death 1-ligand 1 (PD-L1) at ovarian cancer locations in mice. PEI-siRNA uptake transformed these from immunosuppressive cells to efficient antigen-presenting activated tumor-reactive lymphocytes...
Tumors can induce generation and accumulation of the immunosuppressive cells such as regulatory T in tumor microenvironment, contributing to escape from immunological attack. Although dendritic cell (DC)-based cancer vaccine initiate antitumor immune response, DC subsets involved tolerance induction attracted much attention recently. Our previous studies demonstrate that stromal microenvironment spleen, lung, liver program CD11c(low)CD11b(high)Ia(low) DCs with function (CD11b(high)Ia(low)...
Abstract Mast cells are phenotypically and functionally heterogeneous, their state is possibly controlled by local microenvironment. Therefore, specific analyses needed to understand whether mast function as powerful participants or dispensable bystanders in diseases. Here, we show that degranulation of inflammatory synovial tissues patients with rheumatoid arthritis (RA) induced via MAS-related G protein-coupled receptor X2 (MRGPRX2), the expression MHC class II costimulatory molecules on...
IL-10+ regulatory B (Bregs), CD4+Foxp3+ T (Tregs), and CD4+CXCR5+Foxp3+ follicular (TFR) cells regulate the progression of infection disease. This study aimed at examining how those associated with development chronic hepatitis (CHB) C (CHC) in a Chinese population.The numbers circulating Bregs, Tregs TFR 31 CHC, 58 CHB patients 22 healthy controls (HC) were examined by flow cytometry. The potential association clinical measures was analyzed.The CD5+CD19+CD1dhighIL-10+ levels serum IL-10,...
BackgroundNo effective approaches to target mutant Kras have yet been developed. Immunoprevention using KRAS-specific antigenic peptides trigger T cells capable of targeting tumor relies heavily on lipid metabolism. To facilitate better TCR/peptide/MHC interactions that result in cancer preventive efficacy, we combined KVax with avasimibe, a specific ACAT1 inhibitor, tested their anti-cancer efficacy mouse lung models, where mutation was induced before vaccination.MethodsControl growth...
To determine the association between low molecular weight heparin (LMWH) use and mortality in hospitalized COVID-19 patients. We conducted a retrospective study of patients consecutively enrolled from two major academic hospitals exclusively for Wuhan, China, January 26, 2020, to March 2020. The primary outcome was adjusted in-hospital LMWH group compared with non-LMWH using propensity score. Overall, 525 median age 64 years (IQR 19), 49.33% men. Among these, 120 (22.86%) were treated LMWH....
It is well known that some pathogenic cells have enhanced glycolysis; the regulatory network leading to increased glycolysis are not characterized. In this study, we show CNS-infiltrated TH17 from diseased mice specifically upregulate glycolytic pathway genes compared with homeostatic intestinal cells. Bioenergetic assay and metabolomics analyses indicate in vitro-derived highly nonpathogenic Chromatin landscape demonstrate vivo distinct chromatin states, accessibility at NF-κB binding...
A series of novel 2-piperidinopiperidine thiadiazoles were synthesized and evaluated as new leads histamine H3 receptor antagonists. The 4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2-(pyridin-2-yl)morpholine (5u) displayed excellent potency ex vivo occupancy. Compound 5u was also in for antidiabetic efficacy STZ diet-induced obesity type 2 diabetic mice or 12 days. Non-fasting glucose levels significantly reduced compared with vehicle-treated mice. In addition, dose dependently...
Dendritic cells (DCs) play a key role in the natural recognition of pathogens and subsequent activation adaptive immune responses due to their potent antigen-presenting ability. cell-targeting peptide (DCpep) is strongly targeted DCs, which often express antigens, enhance efficacy vaccines. Our previous study showed that recombinant Lactobacillus expressing human DCpep could significantly induce stronger than without DCpep, but mechanism remains unclear. In this study, by enhances response...
This study was designed to investigate the inhibitory effects of Angelica polysaccharide (AP) on activation mast cells and its possible molecular mechanism. In our study, we determined proinflammatory cytokines allergic mediators in anti‐DNP IgE stimulated RBL‐2H3 found that AP (50, 100, 200 μ g/mL) significantly decreased release histamine, β ‐hexosaminidase, leukotrienes C4 (LTC4), IL‐1, IL‐4, TNF‐ α , IL‐6, human monocyte chemotactic protein‐1 (MCP‐1/CCL2) ( p < 0.05). addition, Ca 2+...
We established the physiological relevance of LL-37 induced bladder inflammation. hypothesized that 1) human urinary is increased in pediatric patients with spina bifida, 2) inflammation occurs our mouse model via urothelial binding and dose dependent 3) involves mast cells.To test first hypothesis, we obtained urine samples from 56 bifida 22 normal patients. was measured by enzyme-linked immunosorbent assay. Our second hypothesis tested C57Bl/6 mice challenged 7 concentrations...
Dendritic cell-based vaccine therapy for renal cell carcinoma is effective but requires improvement. Here we explored whether combination with dendritic and anti-CD69 antibody can enhance antitumor efficacy in carcinoma-bearing mice.Balb/c mice were challenged subcutaneously murine (Renca) cells. On day 3 after tumor inoculation, tumor-bearing either left untreated or treated Renca lysate-pulsed cells (i.e. vaccine), antibody, a of antibody. The sacrificed on 28. Tumor volume was measured...
Problem The development of a new and suitable contraceptive methods, as well in‐depth systematic research into underlying mechanisms, is crucial. IZUMO 1 plays an important role in the fusion sperm ovum during fertilization. Izumo −/− mice are infertile. Therefore, may be potential target for vaccine. Method study Linear B‐cell epitopes ( BCE ) were identified using biosynthetic peptides used to immunize female mice. Results Five identified: DLVLDCL 177‐183, YSFYRV 196‐201 (named ‐2), YLT...
Tumor cell lysate (TCL) has an advantage of containing extensive repertoire tumor antigens but requires proper adjuvants to enhance its immunogenicity when used as efficient vaccine. Mycobacterium tuberculosis–derived heat shock protein 70 (TBHsp70) been shown assist crosspresentation exogenously applied and activate innate immunity against cells. In this study, TBHsp70-B16TCL, a preparation generated by mixing recombinant TBHsp70 TCL B16 melanoma cells directly, was tested for The...
Rheumatoid arthritis (RA) is a systemic autoimmune disease that results in chronic and inflammatory disorder. Dynamic balance of helper T cells (Th) 1 17 regulatory (Treg) broken RA. Since there no cure for RA at present, it necessary to find truly effective convenient treatment. Several studies have intended induce ergotopic regulation treat diseases. This study was undertaken potential ergotope peptides investigate their effects treating the animal model underlying mechanisms. First, we...
Staging of lower extremity lymphedema (LEL) is difficult in clinical work. The excellent soft tissue contrast magnetic resonance imaging (MRI) enables changes to be feasibly and reproducibly characterized distinguished.To determine whether cross-section areas edema calves measured with MRI could useful for staging the LEL.We recruited 138 participants who were clinically diagnosed LEL: 61 unilateral 77 bilateral LEL. Two legs underwent different stages Fourteen characters are without LEL; we...
The worsening progress of coronavirus disease 2019 (COVID-19) is attributed to the proinflammatory state, leading increased mortality. Statin works with its anti-inflammatory effects and may attenuate COVID-19. COVID-19 patients were retrospectively enrolled from two academic hospitals in Wuhan, China, 01/26/2020 03/26/2020. Adjusted in-hospital mortality was compared between statin non-statin group by CHD status using multivariable Cox regression model after propensity score matching. Our...