A5002650985- Atherosclerosis and Cardiovascular Diseases
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Congenital heart defects research
- Genetic Associations and Epidemiology
- RNA and protein synthesis mechanisms
- Apelin-related biomedical research
- Williams Syndrome Research
- RNA Interference and Gene Delivery
- Nuclear Receptors and Signaling
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Angiogenesis and VEGF in Cancer
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Hormonal Regulation and Hypertension
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Cancer Genomics and Diagnostics
- Science, Research, and Medicine
- Receptor Mechanisms and Signaling
University of Eastern Finland
2020-2024
University of Tartu
2013-2021
[Figure: see text].
Coronary artery disease (CAD) is a pandemic where up to half of the risk explained by genetic factors. Advanced insights into basis CAD require deeper understanding contributions different cell types, molecular pathways, and genes heritability. Here, we investigate biological diversity atherosclerosis-associated states interrogate their contribution using single-cell bulk RNA sequencing (RNA-seq) mouse human lesions. We identified 12 disease-associated that characterized further gene set...
Abstract Background: Vascular smooth muscle cells (VSMC) exhibit phenotypic plasticity in atherosclerotic plaques, and among other approaches, has been modeled vitro by cholesterol loading. Methods: Meta-analysis of scRNA-seq data from VSMC lineage traced across five experiments murine atherosclerosis was performed. In vivo expression profiles were compared to three datasets VSMCs loaded with polarized macrophages. Results: We identified 24 cell clusters the meta-analysis single mouse...
The human WBSCR22 protein was previously shown to be up-regulated in invasive breast cancer and its ectopic expression enhances tumor cell survival the vasculature. In current study, we show that is important for growth. Knock-down of with siRNA results slower growth WBSCR22-depleted cells. Treatment siWBSCR22 causes defects processing pre-rRNAs reduces level free 40S ribosomal subunit, suggesting involved ribosome small subunit biosynthesis. partially complements yeast homologue, bud23...
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the function synthesis independent of its enzymatic activity. In this work, we studied interaction partners by SILAC-coupled co-immunoprecipitation assay identified TRMT112 as partner WBSCR22. Knock-down expression decreased level mammalian cells, suggesting stability regulated through with TRMT112. localization determined WBSCR22,...
[Figure: see text].
Methylation is an essential epigenetic modification mainly catalysed by S-Adenosyl methionine-dependent methyltransferases (MTases). Several MTases require a cofactor for their metabolic stability and enzymatic activity. TRMT112 small evolutionary conserved protein that acts as co-factor activator different involved in rRNA, tRNA methylation. Using SILAC screen, we pulled down seven methyltransferases-N6AMT1, WBSCR22, METTL5, ALKBH8, THUMPD2, THUMPD3 TRMT11-as interaction partners of...
CALCRL (calcitonin receptor-like) protein is an important mediator of the endothelial fluid shear stress response, which associated with genetic risk coronary artery disease. In this study, we functionally characterized noncoding regulatory elements carrying disease that risks single-nucleotide polymorphisms and studied their role in regulation
Methylation is a widespread modification occurring in DNA, RNA and proteins. The N6AMT1 (HEMK2) protein has DNA N6-methyladenine as well the glutamine histone lysine methyltransferase activities. human genome encodes two different isoforms of N6AMT1, major isoform alternatively spliced isoform, where substrate binding motif missing. Several methyltransferases involved ribosome biogenesis, tRNA methylation translation interact with common partner, TRMT112 protein. In this study, we show that...
Abstract Extracellular vesicles are membraneous particles released by a variety of cells into the extracellular microenvironment. Retroviruses utilize cellular vesiculation pathway for virus budding/assembly and retrovirus Gag protein induces spontaneous formation microvesicles or virus-like (VLPs) when expressed in mammalian cells. In this study, five different melanoma antigens, MAGEA4, MAGEA10, MART1, TRP1 MCAM, were incorporated VLPs their localization within was determined. Our data...
ABSTRACT Coronary artery disease (CAD) is one of the major causes mortality worldwide. Recent genome-wide association studies have started to unravel genetic architecture disease. Such efforts identified Calcitonin receptor-like (CALCRL), an important mediator endothelial fluid shear stress response, associated with CAD risk variants. In this study we functionally characterized non-coding regulatory elements carrying risks SNPs and studied their role in regulation CALCRL expression cells. We...
Abstract Coronary artery disease (CAD) is a pandemic where up to half of the risk explained by genetic factors. Advanced insights into basis CAD require deeper understanding contributions different cell types, molecular pathways and genes heritability. Here, we investigate biological diversity atherosclerosis associated states interrogate their contribution using single bulk RNA sequencing (RNA-Seq) mouse human lesions. We identified thirteen disease-associated which characterized further...