A5015406681- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant nutrient uptake and metabolism
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Coastal wetland ecosystem dynamics
- Agronomic Practices and Intercropping Systems
- Forest Ecology and Biodiversity Studies
- Microbial Community Ecology and Physiology
- Plant Micronutrient Interactions and Effects
- Mine drainage and remediation techniques
- Agriculture and Rural Development Research
- Cassava research and cyanide
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Geochemistry and Elemental Analysis
- Plant-Microbe Interactions and Immunity
- Phytase and its Applications
- Microbial Fuel Cells and Bioremediation
- Plant and fungal interactions
- Fungal and yeast genetics research
- Plant Taxonomy and Phylogenetics
- Bacterial Genetics and Biotechnology
- Genetics, Bioinformatics, and Biomedical Research
- Agricultural pest management studies
Institut Agro Montpellier
2013-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2012-2024
Institut de Recherche pour le Développement
2012-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Laboratoire des Symbioses Tropicales et Méditerranéennes
2012-2022
Université de Montpellier
2013-2022
Mohammed V University
2016-2022
Laboratoire de Microbiologie et Génétique Moléculaires
2017
University of Geneva
2005-2009
Lund University
2002-2005
Rhizobium sp. strain NGR234 is a unique alphaproteobacterium (order Rhizobiales) that forms nitrogen-fixing nodules with more legumes than any other microsymbiont. We report here the 3.93-Mbp chromosome (cNGR234) encodes most functions required for cellular growth. Few essential are encoded on 2.43-Mbp megaplasmid (pNGR234b), and none present second 0.54-Mbp symbiotic plasmid (pNGR234a). Among many striking features, 6.9-Mbp genome different secretion systems known rhizobia probably...
In order to obtain information on genes specifically expressed in the ectomycorrhizal symbiosis, 3,555 sequence tags (ESTs) were analyzed from a cDNA library constructed ectomycorrhiza formed between basidiomycete Paxillus involutus and birch (Betula pendula). libraries saprophytically growing fungus (3,964 ESTs) axenic plants (2,532 parallel. By clustering all EST obtained, nonredundant set of 2,284 unique transcripts either fungal or plant origin identified. The expression pattern these...
The formation of ectomycorrhizal (ECM) root tissue is characterized by distinct morphological and developmental stages, such as preinfection adhesion, mantle, Hartig net formation. global pattern gene expression during these stages in the birch (Betula pendula)-Paxillus involutus ECM association was analyzed using cDNA microarrays. In comparison with nonsymbiotic conditions, 251 fungal (from a total 1,075) 138 plant (1,074 total) genes were found to be differentially regulated development....
Abstract Background Mesorhizobium metallidurans STM 2683 T and sp. strain 4661 were isolated from nodules of the metallicolous legume Anthyllis vulneraria distant mining spoils. They tolerate unusually high Zinc Cadmium concentrations as compared to other mesorhizobia. This work aims study gene expression profiles associated with or exposure identify genes involved in metal tolerance these two strains interest for mine phytostabilization purposes. Results The draft genomes Mezorhizobium...
Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to root (CFN) are generally not the most efficient fix dinitrogen, resulting in decrease legume crop yields. Here, we investigate differential selection by host plants on Rlv. A collection Rlv was collected nodule trapping from soils at five European sites. Representative genomes were sequenced. In parallel,...
• Functional compartmentation of the extramatrical mycelium ectomycorrhizal (ECM) fungi is considered important for operation ECM associations, although molecular basis poorly characterized. Global gene expression profiles colonizing an ammonium sulphate ((NH4)2SO4) nutrient patch, rhizomorphs and root tips Betula pendula–Paxillus involutus association were compared by cDNA microarray analysis. The patch similar to each other but distinctly different from that mycorrhizal tips. Statistical...
Infection of legumes by Rhizobium sp. NGR234 and subsequent development nitrogen-fixing nodules are dependent on the coordinated actions Nod factors, proteins secreted a type III secretion system (T3SS) modifications to surface polysaccharides. The production these signal molecules is plant flavonoids which trigger regulatory cascade controlled transcriptional activators NodD1, NodD2, SyrM2 TtsI. TtsI known control genes responsible for T3SS function synthesis symbiotically important...
• Responses of the Medicago truncatula-Sinorhizobium interaction to variation in N₂-fixation bacterial partner were investigated. Split-root systems used discriminate between local responses, at site with bacteria, and systemic responses related whole plant N status. The lack acquisition by a half-root system nodulated nonfixing rhizobium triggers compensatory response enabling other N₂-fixing partners compensate limitation. This is mediated stimulation nodule development (number size)...
Nitrogen fixing bacteria isolated from hot arid areas in Asia, Africa and America but diverse leguminous plants have been recently identified as belonging to a possible new species of Ensifer (Sinorhizobium). In this study, 6 strains clade were compared with at the genome-wide level. Their capacities utilize various carbon sources establish symbiotic interaction several examined. Draft genomes selected Morocco (Merzouga desert), Mexico (Baja California) well India (Thar desert) produced....
Rhizobium sp. strain NGR234 produces a flavonoid-inducible rhamnose-rich lipopolysaccharide (LPS) that is important for the nodulation of legumes. Many genes encoding rhamnan part molecule lie between 87 degrees and 110 pNGR234a, symbiotic plasmid NGR234. Computational methods suggest 5 12 open reading frames (ORFs) within this arc are involved in synthesis (and subsequent polymerization) L-rhamnose. Two others probably play roles transport carbohydrates. To evaluate function these ORFs, we...
ABSTRACT Anthyllis vulneraria is a legume associated with nitrogen-fixing rhizobia that together offer an adapted biological material for mine-soil phytostabilization by limiting metal pollution. To find at given site, we evaluated the genetic and phenotypic properties of collection 137 recovered from soils presenting contrasting levels. Zn-Pb mine largely contained metal-tolerant belonging to Mesorhizobium metallidurans or another sister species. All isolates harbored cadA marker gene...
Abstract In symbiotic root nodules of legumes, terminally differentiated rhizobia fix atmospheric N2 producing an NH4+ influx that is assimilated by the plant. The plant, in return, provides photosynthates fuel nitrogen acquisition. Mechanisms responsible for adjustment capacity to plant N demand remain poorly understood. We have investigated role systemic signaling whole-plant on mature N2-fixing model association Medicago truncatula/Sinorhizobium using split-root systems. N-satiety rapidly...
Acacia senegal (L) Willd. and seyal Del. are highly nitrogen-fixing moderately salt tolerant species. In this study we focused on the genetic genomic diversity of mesorhizobia symbionts from diverse origins in Senegal investigated possible correlations between strains, their soil origin, tolerance to salinity. We first performed a multi-locus sequence analysis five markers gene fragments collection 47 strains A. 8 localities. Most (60%) clustered with M. plurifarium type strain ORS 1032T,...
Abstract Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis two organisms exchange carbon nutrients in a specific tissue that is at contact compatible fungus plant. There considerable variation degree host specificity among species strains ectomycorrhizal fungi. In this study, we have for first time shown associated with quantitative differences gene expression, divergence nucleotide sequences symbiosis‐regulated genes. Gene...
Abstract We have examined the variations in gene content and sequence divergence that could be associated with symbiotic adaptations ectomycorrhizal fungus Paxillus involutus closely related species filamentosus . Strains various abilities to form mycorrhizae were analysed by comparative genomic hybridizations using a cDNA microarray containing 1076 putative unique genes of P. involutus. To screen for diverging at an enhanced presumably non‐neutral rate, we implemented simple rate test...
The aim of this study was to identify bacteria present in the nodules legume Hedysarum spinosissimum growing metal-contaminated soils; and test whether these root-nodule are able promote host plant growth enhance their phytostabilization potential.
Abstract In northern Mexico, aridity, salinity and high temperatures limit areas that can be cultivated. To investigate the nature of nitrogen-fixing symbionts Phaseolus filiformis , an adapted wild bean species native to this region, their phylogenies were inferred by MLSA. Most rhizobia recovered belong proposed new Ensifer aridi . Phylogenetic analyses nodC nifH show Mexican isolates carry symbiotic genes acquired through horizontal gene transfer are divergent from those previously...
Here, we report the complete genome sequence of Bradyrhizobium sp. strain ORS3257, which forms efficient symbioses with cowpea, peanut, or groundnut. These genomic data will be useful to identify genes associated symbiotic performance and host compatibility on several legumes, including Aeschynomene species, a Nod-independent type III secretion system (T3SS)-dependent symbiosis can established.
The recently proposed species Ensifer aridi represents an interesting model to study adaptive mechanisms explaining its maintenance under stressful pedo-climatic conditions. To get insights into functions associated with hyperosmotic stress adaptation in E. aridi, we first performed RNAseq profiling of cells grown sub-lethal stresses applied by permeating (NaCl) and non-permeating (PEG8000) solutes that were compared a transcriptome from unstressed bacteria. Then priori approach, consisting...