A5090528268- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Chromosomal and Genetic Variations
- Amino Acid Enzymes and Metabolism
- Algal biology and biofuel production
- Wastewater Treatment and Nitrogen Removal
- Agronomic Practices and Intercropping Systems
- Coastal wetland ecosystem dynamics
- Genomic variations and chromosomal abnormalities
- Nematode management and characterization studies
- Plant Micronutrient Interactions and Effects
- Plant Pathogenic Bacteria Studies
- Contact Dermatitis and Allergies
- Allergic Rhinitis and Sensitization
- Cassava research and cyanide
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
- Food Allergy and Anaphylaxis Research
- DNA and Nucleic Acid Chemistry
- Metabolism and Genetic Disorders
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Plant and soil sciences
Universidad Nacional Autónoma de México
2004-2022
Universidad Autónoma del Estado de Morelos
1987-2018
Universidad de Cantabria
2017
Inserm
2017
Universidad de Navarra
2017
Center for Genomic Science
2007-2011
Instituto de Salud Carlos III
2004
University of Geneva
2000
Hospital Universitari Joan XXIII de Tarragona
1999
Institut Pasteur
1990
Abstract Polysomes involved in ovalbumin synthesis were identified by the binding of 125I-anti-ovalbumin to hen oviduct polysomes. Techniques developed for isolation undegraded polysomes and preparation 125I-γ-globulin free ribonuclease activity. The distribution polysome profile is accordance with size that are expected be synthesizing ovalbumin. Anti-ovalbumin does not react extracted from tissues do synthesize ovalbumin, antibodies directed against a protein synthesized bind These...
The genetic structure of a population nonsymbiotic Rhizobium leguminosarum strains was determined by the electrophoretic mobilities eight metabolic enzymes. Nonsymbiotic were isolated from rhizosphere bean plants and characterized growth on differential media at different temperatures, intrinsic antibiotic resistance, lack homology to nifH probe, their inability form nodules roots. All isolates clustered with R. bv. phaseoli reference did not encompass any other taxa. Their rRNA operon...
Abstract The polyribosomes synthesizing ovalbumin and albumin have been isolated from total oviduct polysomes rat liver polysomes, respectively. isolation was performed using an indirect immunoprecipitation technique which is based on the specific reaction occurs between antibody against a purified native protein nascent peptide chains that protein. A soluble antibody-nascent chain-polysome complex formed by incubating with then precipitated anti-antibody. techniques developed are both...
Rhizobium sp. strain NGR234 is a unique alphaproteobacterium (order Rhizobiales) that forms nitrogen-fixing nodules with more legumes than any other microsymbiont. We report here the 3.93-Mbp chromosome (cNGR234) encodes most functions required for cellular growth. Few essential are encoded on 2.43-Mbp megaplasmid (pNGR234b), and none present second 0.54-Mbp symbiotic plasmid (pNGR234a). Among many striking features, 6.9-Mbp genome different secretion systems known rhizobia probably...
Abstract DNA was synthesized in vitro from a template of purified ovalbumin messenger RNA. The product is complementary to homogeneous sequence specific for ovalbumin. amount hen oviduct and liver measured by hybridization experiments found be 2 copies per diploid genome both tissues, indicating that there no selective amplification genes the tissue specialized obalbumin synthesis.
The complete coding sequence of the nitrogenase reductase gene ( nifH ) is present in three different regions a Rhizobium phaseoli symbiotic plasmid. Homology between two containing sequences extends over 5 kilobases. These turn share 1.3 kilobases homology with third region. nucleotide genes were found to be identical. Site-directed insertion mutagenesis indicated that none indispensable for nitrogen fixation during symbiosis Phaseolus vulgaris . This implies at least reiterated can...
Rhizobium phaseoli CFN299 forms nitrogen-fixing nodules in Phaseolus vulgaris (bean) and Leucaena esculenta. It has three plasmids of 185, 225, 410 kilobases. The 410-kilobase plasmid contains the nitrogenase structural genes. We have transferred these to plasmid-free strain Agrobacterium tumefaciens GMI9023. Transconjugants containing different combinations R. were obtained, they exhaustively purified before nodulation was assayed. Only transconjugants harboring nodulate P. L. Nodules...
Rhizobium leguminosarum bv. phaseoli CFN42 contains six plasmids (pa to pf), and pd has been shown be the symbiotic plasmid. To determine participation of other in cellular functions, we used a positive selection scheme isolate derivatives cured each These were obtained for all except one (pe), which only deleted recovered. In regard symbiosis, found that addition pd, pb is also indispensable nodulation, partly owing presence genes involved lipopolysaccharide synthesis. The contribution pb,...
Several lines of evidence suggest that reiterated sequences in the human genome are targets for nonallelic homologous recombination (NAHR), which facilitates genomic rearrangements. We have used a PCR-based approach to identify breakpoint regions rearranged structures genome. In particular, we identified intrachromosomal identical repeats located reverse orientation, may lead chromosomal inversions. A bioinformatic workflow pathway select appropriate analysis was developed. Three such...
Immunological techniques have been developed for the quantification and isolation of polysomes involved in ovalbumin synthesis. For polysomes, two methods developed; one is based on binding 125I-anti-ovalbumin, other immunoprecipitation polysomes. There a good correlation between relative rate synthesis extent 125I-anti-ovalbumin to ribosomes or amount RNA extracted from polysome immunoprecipitates. With special care exclude ribonuclease activity, can be obtained precipitated an undegraded...
Ovalbumin-synthesizing polysomes were isolated from total oviduct by reaction with anti-ovalbumin antibody and adsorption to a matrix of glutaraldehyde-linked ovalbumin. The RNA immunoadsorbed ovalbumin was then deproteinized selectively adsorbed Millipore filters. Several types experiments indicated the resulting fraction is highly enriched for mRNA.
Experience from different laboratories indicates that Rhizobium strains can generate variability in regard to some phenotypic characteristics such as colony morphology or symbiotic properties. On the other hand, several reports suggest under certain stress conditions genetic manipulations cells present genomic rearrangements. In search of frequent rearrangements, we analyzed three laboratory are not considered cause bacterial populations. DNAs direct descendants a single cell were...
Repeated DNA sequences are a general characteristic of eucaryotic genomes. Although several examples reiteration have been found in procaryotic organisms, only the case archaebacteria Halobacterium halobium and volcanii [C. Sapienza W. F. Doolittle, Nature (London) 295:384-389, 1982], has reported as common genomic feature. The genomes two Rhizobium phaseoli strains, one meliloti strain, Agrobacterium tumefaciens strain were analyzed for presence repetitive DNA. spp. closely related soil...
One remarkable characteristic of the genomes some Rhizobium species is frequent occurrence rearrangements. In instances these rearrangements alter symbiotic properties strains. However, no detailed molecular mechanisms have been proposed for generation To understand involved in formation genome phaseoli, we designed a system which allows positive selection amplification and deletion events. We applied this to investigate stability plasmid R. phaseoli. High-frequency events were detected...
The complete nucleotide sequence of the genome Sinorhizobium meliloti, symbiont alfalfa, was reported in 2001 by an international consortium laboratories. comprises a chromosome 3.65 megabases (Mb) and two megaplasmids, pSymA pSymB, 1.35 Mb 1.68 Mb, respectively. Based on whole genome, we designed pathway consecutive rearrangements leading to novel genomic architectures. In first step obtained derivative strains containing replicons; second strain genetic information one single replicon 6.68...
ABSTRACT Strains of the same bacterial species often show considerable genomic variation. To examine extent such variation in Rhizobium etli , complete genome sequence R. CIAT652 and partial sequences six additional strains having different geographical origins were determined. The compared with each other previously reported CFN42. DNA common to all constituted greater part these genomes localized both chromosome large plasmids. About 700 1,000 kb that did not match CFN42 was unique strain....
Abstract Ovalbumin-synthesizing polysomes formed during incubation of a rabbit reticulocyte cell-free protein-synthesizing system in the presence hen oviduct RNA were precipitated by immunological techniques. Several experiments indicate that reaction is specific for ovalbumin polysomes. Ovalbumin nascent chains and intact mRNA molecules are shown immunoprecipitate.