A5086197358- Sphingolipid Metabolism and Signaling
- Lipid metabolism and disorders
- Extracellular vesicles in disease
- Glycosylation and Glycoproteins Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- MicroRNA in disease regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Corneal Surgery and Treatments
- Immune cells in cancer
- Corneal surgery and disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glaucoma and retinal disorders
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Oral Health Pathology and Treatment
- Lysosomal Storage Disorders Research
- Galectins and Cancer Biology
- Lipid Membrane Structure and Behavior
- Salivary Gland Disorders and Functions
University of Milan
2022-2024
Ospedale San Paolo
2023
Abstract Cholangiocarcinoma (CCA) is a rare cancer characterized by global increasing incidence. Extracellular vesicles (EV) contribute to many of the hallmarks through transfer their cargo molecules. The sphingolipid (SPL) profile intrahepatic CCA (iCCA)-derived EVs was liquid chromatography-tandem mass spectrometry analysis. effect iCCA-derived as mediators inflammation assessed on monocytes flow cytometry. showed downregulation all SPL species. Of note, poorly-differentiated higher...
Abstract N-glycosylation plays a key role in modulating the bioactivity of monoclonal antibodies (mAbs), as well light chain (LC) isotype can influence their physicochemical properties. However, investigating impact such features on mAbs conformational behavior is big challenge, due to very high flexibility these biomolecules. In this work we investigate, by accelerated molecular dynamics (aMD), two commercial immunoglobulins G1 (IgG1), representative κ and λ LCs antibodies, both fucosylated...
Lactosylceramide is necessary for the biosynthesis of almost all classes glycosphingolipids and plays a relevant role in pathways involved neuroinflammation. It synthesized by action galactosyltransferases B4GALT5 B4GALT6, which transfer galactose from UDP-galactose to glucosylceramide. synthase activity was classically determined vitro method based on incorporation radiolabeled followed chromatographic separation quantitation product liquid scintillation counting. Here, we used deuterated...
The last step of ex novo ceramide biosynthesis consists the conversion dihydroceramide into catalyzed by sphingolipid Δ4-desaturase DEGS1. DEGS1 variants were found to be responsible for heterogeneous clinical pictures belonging family hypomyelinating leukodystrophies. To investigate mechanisms making such pathogenic, we designed a procedure efficient detection desaturase activity in vitro using LC-MS/MS and prepared suitable cell model knocking out HEK-293T cells through CRISPR-Cas9 genome...
Angiopoietin-like protein 3 (ANGPTL3) is a plasmatic that plays crucial role in lipoprotein metabolism by inhibiting the lipase (LPL) and endothelial (EL) responsible for hydrolysis of phospholipids on high-density (HDL). Interest developing new pharmacological therapies aimed at ANGPTL3 has been growing due to hypolipidemic antiatherogenic profile observed its absence. The goal this study was silico characterization interaction between EL. Because lack any structural information both...
Background/Objectives: Chronic graft-versus-host disease (cGVHD) and oral lichen planus (LPO) are chronic inflammatory conditions with similar manifestations. This study aimed to assess whether serum salivary cytokines (IL-1α, IL-6, IL-17) could serve as reliable biomarkers for cGVHD. Methods: A prospective cohort was conducted involving cGVHD patients, LPO healthy controls. Cytokine levels in saliva were measured by ELISA compared across the groups using Kruskal–Wallis test. Results: IL-17...
Abstract Cholangiocarcinoma (CCA) is a rare cancer with global increasing incidence. Extracellular vesicles (EV) contribute to many of the hallmarks through transfer their cargo molecules. The sphingolipid (SPL) profile intrahepatic CCA (iCCA)-derived EVs was characterized by liquid chromatography-tandem mass spectrometry analysis. effect iCCA-derived as mediators inflammation assessed on monocytes flow cytometry. showed downregulation all SPL species. Of note, poorly-differentiated higher...