A5063942905- Immune Cell Function and Interaction
- Hepatitis C virus research
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- CAR-T cell therapy research
- Immune cells in cancer
- Hepatitis B Virus Studies
- Liver Diseases and Immunity
- Immunodeficiency and Autoimmune Disorders
- Parvovirus B19 Infection Studies
- Sphingolipid Metabolism and Signaling
- Herpesvirus Infections and Treatments
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Cancer Mechanisms and Therapy
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
Policlinico San Matteo Fondazione
2014-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
University of Pavia
2011-2020
Tongji Hospital
2011
The relationship between severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and host immunity is poorly understood. We performed an extensive analysis of immune responses in 32 patients with COVID-19, some whom succumbed. A control population healthy subjects was included. Patients COVID-19 had altered distribution peripheral blood lymphocytes, increased proportion mature natural killer (NK) cells low T-cell numbers. NK CD8+ T overexpressed immunoglobulin mucin domain-3 (TIM-3)...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an RNA virus responsible for a pandemic that causes asymptomatic or paucisymptomatic infection in most individuals but may be severe interstitial pneumonia, myocarditis, kidney injury, hepatitis, distress (ARDS), multiorgan failure (MOF), and death. 1 Patients with disease (COVID-19) typically show signs of hyperinflammation characterized by exuberant host immune response leading to virus-driven cytokine release syndrome. 2A...
We assessed SARS-CoV-2-specific CD4+ and CD8+ T cell responses in samples from 89 acute COVID-19 patients, utilizing blood collected during the first wave of Italy. The goal study was to examine correlations between early phase comparing mild, moderate, or severe disease outcomes. spike (S) non-S proteins were measured a combined activation-induced marker (AIM) intracellular cytokine staining (ICS) assay. Early SARS-CoV-2 S correlated with milder by both AIM IFNγ ICS readouts. correlation...
Hepatitis C virus (HCV) persistence in the host results from inefficiencies of innate and adaptive immune responses. Most studies addressing role immunity concentrated on peripheral blood (PB) natural killer (NK) cells, whereas only limited information is available intrahepatic (IH) NK cells. We therefore examined phenotypic functional features IH PB cells paired liver biopsy venous samples 70 patients with chronic HCV infection 26 control persons subjected to cholecystectomy for gallstones...
The activating natural cytotoxicity receptor NKp30 is critical for killer (NK) cell function and tumor immune surveillance. receptor‐3 (NCR3) gene transcribed into several splice variants whose physiological relevance still incompletely understood. In this study, we investigated the role of its major ligand B7 homolog 6 (B7‐H6) in patients with hepatocellular carcinoma (HCC). Peripheral blood NK phenotype was skewed toward a defective/exhausted profile decreased frequencies cells expressing...
Abstract Natural Killer (NK) cells play a significant role in the early defense against virus infections and cancer. Recent studies have demonstrated involvement of NK both induction effector phases vaccine‐induced immunity various contexts. However, their shaping immune responses following SARS‐CoV‐2 vaccination remains poorly understood. To address this matter, we conducted comprehensive analysis cell phenotype function unexposed individuals who received BNT162b2 vaccine. We employed...
<h3>Objective</h3> Sialic-acid-binding immunoglobulin-like lectin-7 (Siglec-7) is a natural killer (NK) cell inhibitory receptor associated with NK phenotypic and functional abnormalities in HIV-1 infection. We investigated the significance of NK-expressed serum soluble Siglec-7 relation to ability parameters liver necroinflammation fibrosis chronic HCV <h3>Design</h3> were evaluated 130 166 HCV-infected individuals by flow cytometry ELISA, respectively. degranulation cytokine secretion...
Sialic acid-binding Ig-like lectin-7 (Siglec-7) expression is strongly reduced on natural killer (NK) cells from HIV-1 infected viremic patients. To investigate the mechanism(s) underlying this phenomenon, we hypothesized that Siglec-7 could contribute to infection of CD4pos target following its interaction with envelope (Env) glycoprotein 120 (gp120).The ability bind gp120 Env in a sialic acid-dependent manner facilitates both T and monocyte-derived macrophages (MDMs). Indeed,...
Summary Chronic hepatitis C virus ( HCV ) infection is characterized by persistent B‐cell activation, with enhanced differentiation and reduced proliferative ability. To assess the possible role of in altering subset distribution, we examined ex vivo frequencies inhibitory receptor expression 37 chronic ‐infected patients 25 healthy donors HD ). In addition, determined whether short‐term exposure to culture‐derived cc) resulted skewing and/or activation. There was a statistically significant...
The major histocompatibility complex-class I chain related proteins A and B (MICA/B) is upregulated because of cellular stress MICA/B shedding by cancer cells causes escape from NKG2D recognition favoring the emergence cancers. Cholangiocarcinoma (CCA) a relatively rare, though increasingly prevalent, primary liver characterized late clinical presentation dismal prognosis. We explored NKG2D-MICA/B axis in NK 41 patients with intrahepatic cholangiocarcinoma (iCCA). MICA/B-specific 7C6 mAb was...
ABSTRACT Human Cytomegalovirus (HCMV) transcripts (including UL21.5 mRNA) have been found to be packaged in virions, and their detection plasma may indicate the presence of infectious viral particles. The objective this study was verify whether mRNA detected indeed encapsulated particles, representing an indirect marker active replication. To distinguish between virion‐packaged free‐floating RNA, samples from 22 immunocompromised patients were tested before after ribonuclease (RNAse)...
Immune control of human cytomegalovirus (HCMV) replication is critical in bone marrow and solid organ transplant recipients, where uncontrolled can lead to high mortality. Current commercial immune monitoring tools have several limitations, such as a lack appropriate test cutoff values the inability characterise antigen-specific T cells. The main aim our study was develop new interferon-γ (IFN-γ) release assay (IGRA), easy use, quantify HCMV-specific T-cell response (pp65-IGRA). Secondary...
Natural killer (NK) cells are involved in innate immune responses to viral infections either via direct cytotoxicity which destroys virus-infected or production of immunoregulatory cytokines modulate adaptive immunity and directly inhibit virus replication. These functions mediated by different NK subpopulations, with being generally performed CD56(dim) cells, whereas CD56(bright) mainly cytokine secretion. functional defects usually combined so that impaired degranulation is often...
Natural killer (NK) cells play an important role in virus infection, their action being regulated by several activating and inhibitory receptors. The NKp30 receptor its isoforms have recently emerged as determinants of efficient NK cell responses. We determined the relative proportions patients with chronic hepatitis C (HCV) infection healthy donors (HD). function (degranulation cytokine production) correlations clinical parameters were assessed following unsupervised hierarchical clustering...
Natural killer (NK) cells play a pivotal role in cancer immune surveillance, and activating the receptor/ligand interaction may contribute to control development evolution of hepatocellular carcinoma (HCC). We investigated natural group 2 member D (NKG2D) receptor its ligand, major histocompatibility complex class I chain-related protein A B (MICA/B) patients with cirrhosis HCC subjected surgical resection, no HCC, healthy donors (HD). The NKG2D-mediated function was determined peripheral...
Abstract The relationship between SARS-CoV-2 and host immunity is unknown. We show here that patients with COVID-19 had an altered immune phenotype, expansion of adaptive FceRIg neg NK cells, inflammatory CD14 + CD16 monocytes. T cells were reduced overexpressed the Tim-3 exhaustion molecule. Low frequencies CD8 NKG2A mature CD57 associated poor prognosis. These findings unveil a unique immunological profile in patients.
Natural killer (NK) cells are emerging as unique players in the immune response against cancer; however, only limited data available on tumor infiltrating NK head and neck squamous cell carcinoma (HNSCC), one of most common cancer. Occurrence HNSCC is closely related to microenvironment, immunotherapy increasingly being applied this setting. However, success type treatment calls for further investigation field. Surgical HNSSC specimens 32 consecutive patients were mechanically enzymatically...
Background and Aims Current evidence suggests that dysfunctional natural killer (NK) cell responses during hepatitis C virus (HCV) infection can be restored after viral eradication with direct acting antivirals (DAAs). However, the fate of recently described adaptive NK population, endowed increased ability to mediate antibody‐dependent cell‐mediated cytotoxicity (ADCC), HCV is poorly defined, while no study has explored effects DAAs on this subset. Approach Results We performed multicolor...
Abstract Cholangiocarcinoma (CCA) is a rare cancer characterized by global increasing incidence. Extracellular vesicles (EV) contribute to many of the hallmarks through transfer their cargo molecules. The sphingolipid (SPL) profile intrahepatic CCA (iCCA)-derived EVs was liquid chromatography-tandem mass spectrometry analysis. effect iCCA-derived as mediators inflammation assessed on monocytes flow cytometry. showed downregulation all SPL species. Of note, poorly-differentiated higher...
Olfactory and taste disorders (OTD) are commonly found as presenting symptoms of SARS-CoV-2 infection in patients with clinically mild COVID-19. Virus-specific T cells thought to play an important role the clearance SARS-CoV-2; therefore study cell specific immune responses may help understand their possible protection from severe disease. We evaluated SARS-CoV-2-specific four different peptide megapools covering all proteins during acute phase disease 33 individuals or no other symptom...