A5085971614- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- COVID-19 and healthcare impacts
- Immune Cell Function and Interaction
- COVID-19 Impact on Reproduction
- Vaccine Coverage and Hesitancy
- Polyomavirus and related diseases
- Immunodeficiency and Autoimmune Disorders
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- Toxoplasma gondii Research Studies
- Transplantation: Methods and Outcomes
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- HIV Research and Treatment
- Infection Control and Ventilation
- Pregnancy and Medication Impact
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
Policlinico San Matteo Fondazione
2018-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2025
University of Insubria
2023
Tecnologie Avanzate (Italy)
2021
Letermovir (LTV), recently approved for the prophylaxis of human cytomegalovirus (HCMV) reactivation after hematopoietic stem cell transplantation (HSCT), has been shown to decrease rate infection in first months post-transplantation. The aim this study was evaluate impact LTV on immune reconstitution and late-onset infection. We studied HCMV HCMV-specific T 2 matched groups HSCT recipients, those treated with (n = 30; group) receiving preemptive therapy 31; PET group). analyzed rates...
•In SARS-CoV-2-naïve subjects, spike-specific T-cell response was significantly increased after the second dose of vaccine•In patients treated with chemo-immunotherapy, seems to be lower borderline statistical significance•Between naïve and experienced patients, no differences were reported in terms CD4- CD8-specific response•In level SARS-CoV-2 neutralizing antibodies reached a higher median 1 : 80 dose•No COVID-19 cases documented throughout period study BackgroundVery few cancer enrolled...
To assess the humoral and cell-mediated response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) elicited by mRNA BNT162b2 vaccine in SARS-CoV-2-experienced -naive subjects against a reference strain SARS-CoV-2 variants.The (including neutralizing antibodies) T-cell-mediated 145 healthcare workers (both naive positive for previous infection) were evaluated. In subset of subjects, effect variants on antibody level was also investigated.Overall, 125/127 (98.4%) developed both...
Rhinovirus is one of the most common respiratory viruses, causing both upper and lower tract infections. It affects mainly children could cause prolonged infections, especially in immunocompromised patients. Here we report our data on a 15-month surveillance seasonality circulation Lombardy Region, Italy. All rhinovirus/enterovirus-positive samples were amplified with RT-PCR for VP4-VP2 region to assign correct genotype. The median age RV/EV-positive patients 9 years, range 0-96. RV-A RV-C...
Abstract Background Human cytomegalovirus (HCMV) is the leading infectious cause of congenital disabilities. We designed a prospective study to investigate rate, outcome, and risk factors CMV (cCMV) infection in neonates born immune women, potential need effectiveness hygiene recommendations this population. Methods The was composed 2 sequential parts: an epidemiology (part 1) prevention 2) study. Performance part depended upon cCMV rate >0.4%. Women enrolled 1 did not receive...
Human cytomegalovirus (HCMV) infection remains a major complication for solid organ transplant recipients (SOTRs). The aim of this study was to evaluate the role HCMV-specific T cell immunity measured at time HCMV-DNA peak in predicting spontaneous clearance infection. performance cytokine flow cytometry using infected dendritic cells (CFC-iDC), lysate (CFC-iCL) and pp65 peptide pool (CFC-pp65 pool) as stimuli, well ELISPOT assays (ELISPOT-iCL) (ELISPOT-pp65 pool), analysed. Among 40 SOTRs...
The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample...
Human cytomegalovirus (HCMV) infection represents a significant complication for kidney transplant recipients (KTRs). The goal of this study was to evaluate potential immunological markers at pre-transplant in HCMV-seropositive KTRs predicting HCMV severe reactivation (e.g treated reactivation) during the first year after transplant. Before transplant, lymphocyte count measured whole blood and HCMV-specific T-cell response determined using ELISpot assay stimulation with pp65, IE-1 IE-2...
Immune control of human cytomegalovirus (HCMV) replication is critical in bone marrow and solid organ transplant recipients, where uncontrolled can lead to high mortality. Current commercial immune monitoring tools have several limitations, such as a lack appropriate test cutoff values the inability characterise antigen-specific T cells. The main aim our study was develop new interferon-γ (IFN-γ) release assay (IGRA), easy use, quantify HCMV-specific T-cell response (pp65-IGRA). Secondary...
We compared the characteristics and outcomes of vaccinated nonvaccinated patients hospitalized with COVID-19.We analyzed in a COVID hub during three one-month periods: (i) October 15, 2020-November 2020 (prevaccination peak); (ii) 2021-November 2021 (Delta wave); (iii) December 2021-January 2022 (Omicron wave). To define epidemiologic context, SARS-CoV-2 infection healthcare workers was analyzed.SARS-CoV-2 incidence 146 cases per 1000 persons (prevaccination) 67 (postvaccination, when...
Immune correlates of protection against human cytomegalovirus (HCMV) infection are still debated. This study aimed to investigate which arm the immune response plays a major role in HCMV kidney transplant recipients (n = 40) and heart 12). Overall, patients were divided into 2 groups: one including 37 with low viral load (LVL), other 15 high (HVL). All LVL resolved spontaneously, whereas HVL all treated or more courses antivirals. In patients, DNAemia, was than 100 times higher LVL, appeared...
The durability of immunogenicity SARS-CoV-2 vaccination in cancer patients remains to be elucidated. We prospectively evaluated the vaccine triggering both humoral and cell-mediated immune response treated with anti-programmed cell death protein 1/programmed death-ligand 1 or without chemotherapy 6 months after BNT162b2 vaccine.In previous study, 88 were enrolled, whereas analyses below refer 60 still on immunotherapy at time follow-up. According exposure, classified as SARS-CoV-2-naive...
A relevant proportion of immunocompromised patients did not reach a detectable seroconversion after full primary vaccination cycle against SARS-CoV-2. The effect different immunosuppressants and the potential risks for SARS-CoV-2 infection in these subjects is largely unknown. Patients from Rivalsa prospective, observational cohort study with planned anti third dose mRNA between October December 2021 were asked to participate this follow-up study. about eventual confirmed positivity within 6...
Containment measures have been applied in several countries order to limit the diffusion of SARS-CoV-2 epidemic. The scope this study is analyze evolution first wave epidemic throughout Italy and factors associated different way it spread Italian Regions, starting from day that indigenous cases were detected through 81 (6 days after end strict lockdown). Data obtained daily reports are represented as number (and percentage) cases/100,000 persons. A lockdown with movement restrictions,...
The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) immunocompromised patients is crucial for long-term protection. Immune to SARS-CoV-2 infection was analysed 57 IC 15 solid organ transplanted (TX) patients. Antibody responses were determined by ELISA neutralization assay. T-cell stimulation with peptide pools the Spike, Envelope, Membrane, Nucleocapsid proteins a 20-h Activation Induced Marker (AIM) 7-day lymphoproliferative assays. detected at...
Abstract Human cytomegalovirus (HCMV) infection represents a major complication for solid organ transplant recipients. The aim of this study was to verify if the measurement HCMV‐specific T‐cells could help identify patients protected against HCMV disease cytokine flow cytometry using infected dendritic cells as stimulus (CFC‐iDC, which discriminates between CD4 + and CD8 T cells), ELISPOT, cell lysate (ELISPOT‐iCL) or pp65 (ELISPOT‐pp65) stimulus, were adopted. Among 47 kidney recipients...
Abstract Postnatal human cytomegalovirus (HCMV) infection in newborns is well characterized for preterm infants but less so term infants. We sought to analyze the rates and routes of HCMV transmission full‐term during first year life. A cohort 120 seropositive mothers their 122 were tested after delivery HCMV‐DNA shedding different bodily fluids. was defined as detection >2.5 × 10 2 copies/mL infants' saliva swabs. Maternal neutralizing antibody serum titer, HCMV‐specific T‐cell response,...
The immunogenicity of severe acute respiratory syndrome 2 virus (SARS-CoV-2) vaccines in immunocompromised patients remains to be further explored. Here, we evaluated the elicited by complete vaccination with BNT162b2 vaccine solid organ transplant recipients (SOTRs). A cohort 110 SOTRs from Northern Italy were vaccinated two doses mRNA and prospectively monitored at baseline after 42 days. Both SARS-CoV-2 naïve recovered subjects included. Humoral response vaccination, including...
SARS-CoV-2 still represents a global health burden, causing more than six million deaths worldwide. Moreover, the emergence of new variants has posed issues in terms vaccine efficacy and immunogenicity. In this study, we aimed to evaluate neutralizing antibody response against different cohorts vaccinated unvaccinated subjects. Four-fold diluted sera from naïve recovered subjects with two or three doses BNT162b2 were challenged 14 variants, titer was measured. Results compared those obtained...
In the setting of infectious diseases, antibodies show different functions beyond neutralizing activity. this study, we investigated activation NK cells in vitro presence human cytomegalovirus (HCMV)-specific and their potential role control HCMV infection through antibody-dependent cell cytotoxicity (ADCC). Retinal pigmented epithelial (ARPE-19) infected with strain VR1814 were co-cultured cytokine-activated peripheral blood mononuclear (PBMCs) sera collected from 23 HCMV-seropositive 9...
Pregnant women are more susceptible to severe disease associated with SARS-CoV-2 infection. We performed a prospective study analyze the inflammatory and immune profile after infection occurring in vaccinated or non-vaccinated pregnant their newborns.Twenty-five were enrolled, sixteen cord blood samples obtained at delivery.We observed that IL-1β, TNF-α, Eotaxin, MIB-1β, VEGF, IL-15, IL-2, IL-5, IL-9, IL-10 IL-1ra levels significantly higher than mothers. Furthermore, newborns of mothers...