A5093936732- Sphingolipid Metabolism and Signaling
- GABA and Rice Research
- Lysosomal Storage Disorders Research
- Coffee research and impacts
- Lipid Membrane Structure and Behavior
- Cannabis and Cannabinoid Research
University of Milan
2023-2024
Cannabis as a therapeutic agent is increasing in popularity all around the globe, particularly Western countries, and its potential now well assessed. On other hand, each country has own regulation for preparation of cannabis macerated oils; Italy, there are only few methods allowed. With this work, we aim to perform stability study oils produced with novel method extraction cannabinoids from inflorescence. Three different varieties were used, without adding tocopherol acetate an...
The last step of ex novo ceramide biosynthesis consists the conversion dihydroceramide into catalyzed by sphingolipid Δ4-desaturase DEGS1. DEGS1 variants were found to be responsible for heterogeneous clinical pictures belonging family hypomyelinating leukodystrophies. To investigate mechanisms making such pathogenic, we designed a procedure efficient detection desaturase activity in vitro using LC-MS/MS and prepared suitable cell model knocking out HEK-293T cells through CRISPR-Cas9 genome...