A5101791854- Immunodeficiency and Autoimmune Disorders
- Peptidase Inhibition and Analysis
- NF-κB Signaling Pathways
- interferon and immune responses
- Diabetes and associated disorders
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Blood disorders and treatments
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Genetic Syndromes and Imprinting
- Antifungal resistance and susceptibility
- Complement system in diseases
- Tuberculosis Research and Epidemiology
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Cellular Mechanics and Interactions
- Food Quality and Safety Studies
- T-cell and B-cell Immunology
- Brucella: diagnosis, epidemiology, treatment
- Platelet Disorders and Treatments
- Bartonella species infections research
- Plant Disease Resistance and Genetics
Second Affiliated Hospital of Guangzhou Medical University
2025
Guangzhou Medical University
2025
Shenzhen Children's Hospital
2004-2024
Chinese Academy of Sciences
2012
Institute of Geochemistry
2012
Ministry of Education of the People's Republic of China
2007
Background: COPD is a healthcare problem. However, the underlying mechanism remains unclear. Our study aimed to explore key genes involved in immune infiltration using bioinformatic tools. Methods: In this study, scRNA-seq analysis was utilized specific marker of each cell subtype COPD. TSNE used evaluate relationship between cluster. Lasso regression identified 21 as characteristics modulated by single-cell NK subpopulation. The "limma" package for differentially expressed analysis....
Abstract Purpose Talaromyces marneffei (TM) is an opportunistic fungus leading to multi-organ damages and poor prognosis in immunocompromised individuals. TM infections children are rare our knowledge infection insufficient. To investigate the clinical characteristics of TM-infected explore underlying mechanisms for host against TM, we analysed patients diagnosed hospital. Methods Eight with have been identified Shenzhen Children’s Hospital during 2017–2021. Clinical data were collected from...
Autoinflammation and phospholipase C (PLC) γ2-associated antibody deficiency immune dysregulation (APLAID) syndrome is an autoinflammatory disease caused by gain-of-function variants in PLCG2. This study investigates the pathogenic mechanism of a novel variant PLCG2 patient with APLAID syndrome.
Abstract Background Primary immunodeficiency disorders ( PID ) is a group of heterogeneous diseases mainly characterized by severe and recurrent infections an increased susceptibility to lymphoproliferative, atopic, autoimmune conditions. The clinical diagnosis should preferably be complemented genetic diagnosis. To date, ‐related reports from China seldom attempt make test for their patients. Methods Our study aimed evaluate demographic data, manifestations, molecular patients southern...
To explore the clinical phenotype, genetic variant, treatment and prognosis of a child with mosaic variegated aneuploidy syndrome (MVAS).Immunological marker screening, chromosomal karyotyping whole exome sequencing were carried out.The 1-year-11-month old girl has featured severe growth retardation, feeding difficulty, short stature, microcephaly, facial anomalies, scoliosis, visual impairment, hypotonia, chylothorax, renal lesions. Karyotype analysis peripheral blood lymphocytes discovered...
Objective The X-linked form of the chronic granulomatous disease(X-CGD)arises from mutations in CYBB gene, which encodes 91-KD glycoprotein gp91phox, component flavocytochrome b558. We confirm a X-CGD case recently and explore mutation gene this family. Methods Clinical features laboratory data were collected pedigree with proband was hospitalized Shenzhen Children Hospital. All exon exon-intron boundaries including 3 family members analyzed by direct sequencing PCR product genomic...
Objective To investigate the changes of CD4~+ T cell subset in role immuno-pathogenesis type 1 diabetes mellitus(T1DM). Methods Real-time PCR was used to evaluate ex-pression levels transcriptional factors (T-bet, GATA-3, Foxp3, ROR-γt), cytokines (IFN-γ, IL-4, IL-10, IL-17A) and negative regulatory (CTLA-4, GITR) mRNA from cells. The proportions Th1, Th2, Tr Th17 cells peripheral blood were detected by flow cytometric analysis. Plasma cyto-kine TGF-β IL-6) concentrations measured...
Objective: To explore the clinical phenotype, immunological features, pathogenesis and gene variation of a case with A20 haploinsufficiency (HA20). Methods: A patient diagnosed tumor necrosis factor α-induced protein 3 (TNFAIP3) mutated HA20 was admitted into Shenzhen Children's Hospital in May,2019.The data analyzed. Flow cytometry used to detect patient's peripheral blood lymphocyte subsets, also, percentage follicular helper T cell (TFH) cells thirteen healthy controls. After construction...
Objective To investigate the effect of lncRNA CCAT1 and miR-130b-3p on radiosensitivity human pancreatic cancer cells PANC-1. Methods Real-time PCR was used to detect relative expression levels in tissues cell lines including PANC-1 irradiated with 2 Gy X-rays. After silencing and/or inhibiting expression, apoptosis rate, Caspase 3 activity survival were detected by flow cytometry, detection kit colony formation assay, respectively. Cell curve stimulated multi-target single-hit model....