A5000867962- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Planarian Biology and Electrostimulation
- Phagocytosis and Immune Regulation
- Advanced Fluorescence Microscopy Techniques
- Axon Guidance and Neuronal Signaling
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Proteoglycans and glycosaminoglycans research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Bone and Dental Protein Studies
- Muscle Physiology and Disorders
University of Zurich
2019-2024
Vollum Institute
2022
Oregon Health & Science University
2022
European Molecular Biology Laboratory
2005-2020
European Molecular Biology Laboratory
2009-2020
European Molecular Biology Organization
2008-2015
European Bioinformatics Institute
2010-2014
Max Planck Institute for Developmental Biology
1999-2008
Max Planck Society
2002
University of Cambridge
1998
The collective migration of cells in the form cohesive tissues is a hallmark both morphogenesis and repair. extrinsic cues that direct these complex migrations usually act by regulating dynamics specific subset cells, those at leading edge. Given normally function tissue to lay down multicellular structures, such as branched epithelial networks or sensory organs, it surprising how little known about mechanisms organize behind Cells zebrafish lateral line primordium switch from...
Although cells migrate in a constrained 3D environment vivo, in-vitro studies have mainly focused on the analysis of moving 2D substrates. Under such conditions, Golgi complex is always located towards leading edge cell, suggesting that it involved directional movement. However, several lines evidence indicate this location can vary depending cell type, or developmental processes. We used micro contact printing (microCP) to study migration geometrically shape within polarized phenotype....
The directed migration of cell collectives drives the formation complex organ systems. A characteristic feature many migrating is a ‘tissue-scale’ polarity, whereby ‘leader’ cells at edge tissue guide trailing ‘followers’ that become assembled into polarised epithelial tissues en route. Here, we combine quantitative imaging and perturbation approaches to investigate state transitions during collective organogenesis, using zebrafish lateral line primordium as an in vivo model. readout...
During brain development, many newborn neurons undergo apoptosis and are engulfed by microglia, the tissue-resident phagocytes of brain, in a process known as efferocytosis. A hallmark microglia is their highly branched morphology characterized presence numerous dynamic extensions that these cells use for scanning parenchyma engulfing unwanted material. The mechanisms driving branch formation apoptotic cell engulfment unclear. By taking live-imaging approach zebrafish, we show while generate...
How cell collectives move and deposit subunits within a developing embryo is question of outstanding interest. In many cases, chemotactic mechanism employed, where cells up or down previously generated attractive repulsive gradient signalling molecules. Recent studies revealed the existence systems with isotropic chemoattractant expression in lateral line primordium zebrafish. Here we propose for collective, which actively modulates an isotropically expressed ligand encodes initial symmetry...
Quantitative microscopy is becoming increasingly crucial in efforts to disentangle the complexity of organogenesis, yet adoption potent new toolbox provided by modern data science has been slow, primarily because it often not directly applicable developmental imaging data. We tackle this issue with a newly developed algorithm that uses point cloud-based morphometry unpack rich information encoded 3D image into straightforward numerical representation. This enabled us employ tools, including...
Studies on signalling dynamics in living embryos have been limited by a scarcity of vivo reporters. Tandem fluorescent protein timers provide generic method for detecting changes population age and thus readouts events that lead to stability or location. When imaged with quantitative dual-colour fluorescence microscopy, tandem offer detailed ‘snapshot’ activity from subcellular organismal scales, therefore the potential revolutionize studies developing embryos. Here we use computer modelling...