A5024095045- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Metabolism, Diabetes, and Cancer
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
Institute for Research in Immunology and Cancer
2020-2025
Université de Montréal
2020-2025
University of Windsor
2015-2024
Dalhousie University
2022
As a dividing cell exits mitosis and daughter cells enter interphase, many proteins must be dephosphorylated. The protein phosphatase 2A (PP2A) with its B55 regulatory subunit plays crucial role in this transition, but the identity of substrates how their dephosphorylation promotes mitotic exit are largely unknown. We conducted maternal-effect screen Drosophila melanogaster to identify genes that function PP2A-B55/Tws cycle. found eggs receive reduced levels Tws components nuclear envelope...
Mitotic exit requires the dephosphorylation of many proteins whose phosphorylation was needed for mitosis. Protein phosphatase 2A with its B55 regulatory subunit (PP2A-B55) promotes this transition. However, events and substrates that it regulates are incompletely understood. We used proteomic approaches in Drosophila to identify interact dephosphorylated by PP2A-B55. Among several candidates, we identified emerin (otefin ). Emerin resides inner nuclear membrane interacts DNA-binding protein...
In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 (also known as LEM4 in humans) which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions is incompletely understood. Using combination approaches Drosophila...
Polo-like kinase 1 (PLK1) is a serine/threonine required for mitosis and cytokinesis. As cancer cells are often hypersensitive to partial PLK1 inactivation, chemical inhibitors of have been developed tested in clinical trials. However, these small molecule alone not completely effective. promotes numerous molecular cellular events the cell division cycle it unclear which most crucially depend on activity. We used CRISPR-based genome-wide screening strategy identify genes whose inactivation...
In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 (also known as LEM4 in humans) which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions is incompletely understood. Using combination approaches Drosophila...
In mitosis and meiosis, chromosome segregation is triggered by the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit ubiquitin ligase that targets proteins for degradation, leading to separation of chromatids. APC/C activation requires phosphorylation its APC3 APC1 subunits, which allows bind co-activator Cdc20. The identity kinase(s) responsible in vivo unclear. Cyclin B3 (CycB3) an activator Cyclin-Dependent Kinase 1 (Cdk1) required meiotic anaphase flies, worms vertebrates. It...
Meiosis, like mitosis, depends on the activity of cyclin dependent kinase Cdk1 and its partners. Here, we examine specific requirements for three mitotic cyclins, A, B, B3 in meiosis Drosophila melanogaster We find that all cyclins contribute redundantly to nuclear envelope breakdown, though A appears make most important individual contribution. Cyclin is also required biorientation homologs I. B3, as previously reported, anaphase progression I II. it plays a redundant role, with preventing...
Chromosome segregation in meiosis is controlled by a conserved pathway that culminates Separase-mediated cleavage of the α-kleisin Rec8, leading to dissolution cohesin rings. Drosophila has no gene encoding and absence known Separase target raises question whether its regulator Securin (Pim Drosophila) are important meiosis. Here, we investigate role Securin, complex female using fluorescence situ hybridization against centromeric arm-specific sequences monitor cohesion. We show destruction...
Abstract Meiosis is a complex variant of the mitotic cell cycle, and as such relies on many same proteins involved in mitosis, but utilizes these novel ways. As Cdk1 its cyclin partners, Cyclin A, B, B3 are required at multiple steps meiosis. Here, we study effect stabilized forms three cyclins to consequences failure degrade We find that promotes ectopic microtubule polymerization throughout egg, dependent APC/C activity apparently due consequent destruction A B. present data suggests CycB,...
In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 (also known as LEM4 in humans) which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions is incompletely understood. Using combination approaches Drosophila...
ABSTRACT In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions in is incompletely understood. Using combination approaches Drosophila , along structural...
In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions in is incompletely understood. Using combination approaches Drosophila , along structural modeling,...
ABSTRACT In mitosis and meiosis, chromosome segregation is triggered by the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit ubiquitin ligase that targets proteins for degradation, leading to separation of chromatids. APC/C activation requires phosphorylation its APC3 APC1 subunits, which allows bind Cdc20 co-activator. The identity kinase(s) responsible in vivo unclear. Cyclin B3 required meiotic anaphase flies, worms vertebrates, but whether it activates We found Drosophila...