A5008161639- Neurotransmitter Receptor Influence on Behavior
- Genetic Mapping and Diversity in Plants and Animals
- Regulation of Appetite and Obesity
- Decision-Making and Behavioral Economics
- Genetic Associations and Epidemiology
- Peroxisome Proliferator-Activated Receptors
- Genetic and phenotypic traits in livestock
- Mathematics, Computing, and Information Processing
- Nuclear Physics and Applications
- Genetics and Plant Breeding
- Alcohol Consumption and Health Effects
- Mental Health Research Topics
- Genomic variations and chromosomal abnormalities
- Molecular Biology Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Fetal and Pediatric Neurological Disorders
- Research Data Management Practices
- RNA and protein synthesis mechanisms
- Algorithms and Data Compression
- Chemical Synthesis and Analysis
- Prenatal Screening and Diagnostics
- Behavioral Health and Interventions
- Computational Physics and Python Applications
- Gene expression and cancer classification
- Neuropeptides and Animal Physiology
University of California, San Diego
2023-2025
The Ohio State University
2024
Università di Camerino
2024
Medical University of South Carolina
2024
Leeds General Infirmary
2014
University of Leeds
2014
Abstract The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing individual differences in OUD vulnerability. shows strong heritability, however genetic variants vulnerability remain poorly defined. We performed a genome-wide association study using over 850 male and female heterogeneous stock (HS) rats identify genes underlying behaviors associated with such as nociception, well heroin-taking, extinction seeking...
Array comparative genomic hybridization (CGH) has become the technology of choice for high-resolution prenatal whole genome analysis. Limitations microarrays are mainly related to analog nature analysis, and poor-quality DNA can result in failed quality metrics with these platforms. We examined a cohort abnormal fetuses array CGH results using next-generation sequencing algorithm, CNV-Seq. assessed ability platform handle suboptimal samples generate interpretable molecular karyotypes.Nine...
Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While microarrays reference panels imputation available human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing. However, low-coverage whole-genome offers alternative that has several advantages. Here, we describe a...
Delay discounting refers to the behavioral tendency devalue rewards as a function of their delay in receipt. Heightened has been associated with substance use disorders, well multiple co-occurring psychopathologies. Genetic studies humans and animal models have established that is heritable trait, but only few specific genes discounting. Here, we aimed identify novel genetic loci through genome-wide association study (GWAS) using Heterogenous Stock rats, genetically diverse outbred...
Abstract The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing individual differences in OUD vulnerability. shows strong heritability, however genetic variants vulnerability remain poorly defined. We performed a genome-wide association study using over 850 male and female heterogeneous stock (HS) rats identify genes underlying behaviors associated with such as nociception, well heroin-taking, extinction seeking...
Delay discounting refers to the behavioral tendency devalue rewards as a function of their delay in receipt. Heightened has been associated with substance use disorders and multiple co-occurring psychopathologies. Human animal genetic studies have established that is heritable, but only few genes identified. We aimed identify novel loci through genome-wide association study (GWAS) using Heterogeneous Stock (HS) rats, genetically diverse outbred population derived from eight inbred founder...
<title>Abstract</title> The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing individual differences in OUD vulnerability. shows strong heritability, however genetic variants toward vulnerability remain poorly defined. We performed a genome-wide association study using over 850 male and female heterogeneous stock (HS) rats identify genes underlying behaviors associated with such as nociception, well heroin-taking,...
Abstract Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While microarrays reference panels imputation available human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing. However, low-coverage whole-genome offers alternative that has several advantages. Here, we describe a...
This protocol is conducted after a set of libraries are completed and ready to quantify pool. outlines the final steps before submitting for sequencing.
This protocol is designed for Agencourt's DNAdvance Extraction kit on the EPmotion 5075. Samples are extracted in a 96 well plate. continuation of "Sample Cutting/Processing" Protocol
This protocol is designed for Twist 96-PLex Library Prep. We use the EPmotion 5075 to add sample barcodes (can also easily be done manually with a multichannel pipette). continuation of "EPMotion - Normalization and Randomization" protocol.
This protocol normalizes and randomizes samples on the EPmotion for Twist 96-Plex (Formerly riptide) library prep protocol. The procedure outlines how to complete normalization calculations randomization process up 96 samples, as well set EPMotion robot a 96-well plate.
Procedure for preparing a 96 sample plate extraction on the EPmotion 5075 (can also do by hand). This protocol is created Agencourt's DNAdvance Kit.