A5027451254- Gene expression and cancer classification
- Diabetes Management and Research
- Diabetes and associated disorders
- Advanced Proteomics Techniques and Applications
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Effects of Radiation Exposure
- Pancreatic function and diabetes
- Lung Cancer Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Head and Neck Cancer Studies
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Carcinogens and Genotoxicity Assessment
- Gene Regulatory Network Analysis
- COVID-19 diagnosis using AI
- Cancer, Lipids, and Metabolism
- AI in cancer detection
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Long-Term Effects of COVID-19
Silesian University of Technology
2016-2025
University Hospital Heidelberg
2015
Heidelberg University
2015
Stockholm University
2014
University of Silesia in Katowice
2014
Wrocław University of Environmental and Life Sciences
2010
Rice University
2004-2005
Analysis of gene set (GS) enrichment is an essential part functional omics studies. Here, we complement the established evaluation metrics GS algorithms with a novel approach to assess practical reproducibility scientific results obtained from tests when applied related data different studies.We evaluated eight and one algorithm for reproducibility, sensitivity, prioritization, false positive rate computational time. In addition algorithms, also included Coincident Extreme Ranks in Numerical...
We propose a new method that employs transfer learning techniques to effectively correct sampling selection errors introduced by sparse annotations during supervised for automated tumor segmentation. The practicality of current learning-based tissue classification approaches is severely impeded their dependency on manually segmented training databases need be recreated each scenario application, site, or acquisition setup. comprehensive annotation reference datasets can highly...
We analysed the temporal changes in incidence of childhood type 1 diabetes and its demographic determinants Poland from 1989 to 2004, validating model with data 1970 1989. also estimated a predictive trends for near future. Children under 15 years newly diagnosed mellitus drawn seven regional registries were ascertained prospectively using Epidemiology Prevention Diabetes study (EURODIAB) criteria. The rates (IRs) dependency age, sex, seasonality, geographical region population density. Time...
Anatomical lobar resection and mediastinal lymphadenectomy remain the standard for treatment of early stage non-small-cell lung cancer (NSCLC) are preferred over procedures such as segmentectomy or wedge resection. However, there is an ongoing debate concerning influence extent on overall survival. The aim this article was to assess survival different types Stage I NSCLC.We performed a retrospective analysis results surgical NSCLC. Between 1 January 2007 31 December 2013, data from 6905...
To present the incidence trend for Type 1 diabetes in Polish children aged 0-14 years, updated using data collected during 2005-2012, and assess reliability of predictive model constructed previously 1989-2004 database.Children < 15 years with newly diagnosed are recorded prospectively (EURODIAB criteria) several regional registers Poland. Age- gender-standardized rates were calculated per 100 000 persons/year. Incidence analysed terms dependency on age, gender, geographical region...
There exist many methods for describing the complex relation between changes of gene expression in molecular pathways or ontologies under different experimental conditions. Among them, Gene Set Enrichment Analysis seems to be one most commonly used (over 10,000 citations). An important parameter, which could affect final result, is choice a metric ranking genes. Applying default may lead poor results.In this work 28 benchmark data sets were evaluate sensitivity and false positive rate set...
Exosomes and other extracellular vesicles are key players in cell-to-cell communication, it has been proposed that they involved different aspects of the response to ionizing radiation, including transmitting radiation-induced bystander effect mediating radioresistance. The functional role exosomes depends on their molecular cargo, proteome content. Here we aimed establish profile released vitro by irradiated UM-SCC6 cells derived from human head-and-neck cancer identify processes associated...
Abstract Molecular profiling of small extracellular vesicles (sEV) isolated from plasma cancer patients emerges as promising strategy for biomarkers discovery. We investigated the proteomic profiles sEV immunoselected using anti‐CSPG4 antibodies 15 melanoma patients’ plasma. The proteomes separated into cell‐derived (MTEX) and non‐malignant (NMTEX) were compared high‐resolution mass spectrometry. Paired analysis identified MTEX‐associated profile 16 proteins that discriminated MTEX NMETEX....
Background and aims MiniMed 780G is the first Advanced Hybrid Closed Loop (AHCL) system in Poland, approved EU 2020. To date, observations of glycemic control up to 12 months have been published. This study aimed analyze anthropometric parameters children adolescents with type 1 diabetes (T1D) after two years using AHCL system. Materials methods We prospectively collected data, pump, continuous glucose records fifty T1D (9.9 ± 2.4 years, 24 (48%) boys, for 3.9 2.56 years) an compared...
The role of adjuvant chemotherapy in stage T2-T3N0 colon cancer (CC) is controversial and there are currently no reliable factors allowing for individual selection patients with high risk relapse such therapy. We searched microRNA-based signature prognostic significance this group. assessed by qRT-PCR expression 754 microRNAs (miRNAs) tumour samples from 85 pT2-3N0 CC treated surgery alone. MiRNA was compared between two groups patients: 40 45 who did not develop distant metastases after...
Type and quality of sample preparation have significant impact on imaging mass spectrometry results. Though fresh‐frozen tissues is considered to give the best results, they are incompatible with clinical practice, since routine diagnostics most frequently performed using formalin‐fixed tissues, paraffin‐embedded material a gold standard in histopathology. We aimed assess utility tissue specimen processed without paraffin embedding (i.e., deep‐frozen cryo‐sectioned) for MALDI both peptides...
Aging | doi:10.18632/aging.203399. Aleksandra Pisarek, Ewelina Pośpiech, Antonia Heidegger, Catarina Xavier, Anna Papież, Danuta Piniewska-Róg, Vivian Kalamara, Ramya Potabattula, Michał Bochenek, Marta Sikora-Polaczek, Aneta Macur, Woźniak, Jarosław Janeczko, Christopher Phillips, Thomas Haaf, Joanna Polańska, Walther Parson, Manfred Kayser, Wojciech Branicki
Information on the influence of insulin treatment using advanced hybrid closed loop systems (AHCL) body weight young patients with type 1 diabetes (T1D) is scarce. The aim this study was to observe whether there were any changes in mass index (BMI) children and adolescents T1D treated Medtronic Minimed 780G AHCL after year follow up analyze potential associations between these doses.For 50 (age 5.4-16.8 years, 24 (48%) boys, for 3.9 ± 2.56 years) an system anthropometric data collected...
A population study is reported in which the DNA damage induced by g-radiation (2 Gy) and kinetics of subsequent repair were estimated comet micronucleus assays isolated lymphocytes 82 healthy donors patients with head neck cancer before radiotherapy. The parameters background radiation-induced damage, rate repair, residual non-repaired measured assay, for every donor computer-fitted to an exponential curve. level irradiation assay as well micronuclei significantly higher patient group than...
Abstract Background Mass spectrometric analysis of the blood proteome is an emerging method clinical proteomics. The approach exploiting multi-protein/peptide sets (fingerprints) detected by mass spectrometry that reflect overall features a specimen's proteome, termed pattern analysis, have been already shown in several studies to applicability cancer diagnostics. We aimed identify serum patterns specific for early stage breast patients using MALDI-ToF spectrometry. Methods Blood samples...
DNA microarrays are used for discovery of genes expressed differentially between various biological conditions. In microarray experiments the number analyzed samples is often much lower than (probe sets) which leads to many false discoveries. Multiple testing correction methods control discoveries but decrease sensitivity discovering genes. Concerning this problem, filtering improving power detection were proposed in earlier papers. These techniques two-step procedures, where first step some...
The video-assisted thoracoscopic surgery (VATS) approach has become a standard for the treatment of early-stage non-small-cell lung cancer (NSCLC). Recently published meta-analyses proved benefit VATS versus thoracotomy overall survival (OS) and reduction postoperative complications. aim this study was to compare early outcomes, long-term rate complications thoracotomy.In retrospective cohort study, we analysed 982 individuals who underwent surgical resection Stage I-IIA NSCLC between 2007...
Background Graves' disease (GD) is a complex in which genetic predisposition modified by environmental factors. The aim of the study was to examine association between variants genes encoding proteins involved immune response and age at diagnosis GD. Methods 735 GD patients 1216 healthy controls from Poland were included into study. Eight HLA-DRB1, TNF, CTLA4, CD40, NFKb, PTPN22, IL4 IL10 genotyped. Patients stratified with genotype analysed. Results Polymorphism TNF CTLA4 associated...
Mixture - modeling of mass spectra is an approach with many potential applications including peak detection and quantification, smoothing, de-noising, feature extraction spectral signal compression. However, existing algorithms do not allow for automated analyses whole spectra. Therefore, despite highlighting advantages mixture peptide/protein mixtures some preliminary results presented in several papers, the was so far developed to stage enabling systematic comparisons software packages...