A5000784511- Gut microbiota and health
- Probiotics and Fermented Foods
- Fermentation and Sensory Analysis
- Diet and metabolism studies
- Microbial Metabolites in Food Biotechnology
- Meat and Animal Product Quality
- Infant Nutrition and Health
- Metabolomics and Mass Spectrometry Studies
- Food Allergy and Anaphylaxis Research
- Horticultural and Viticultural Research
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Food composition and properties
- Birth, Development, and Health
- Food Industry and Aquatic Biology
- Liver Disease Diagnosis and Treatment
- Infant Health and Development
- Food Quality and Safety Studies
- Child Nutrition and Feeding Issues
- Cystic Fibrosis Research Advances
- Microbial Inactivation Methods
- Autism Spectrum Disorder Research
- Fatty Acid Research and Health
- Gestational Diabetes Research and Management
- Advanced Chemical Sensor Technologies
Bambino Gesù Children's Hospital
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2025
Vatican Secret Archives
2016-2019
University of Bologna
2008-2017
University of Rome Tor Vergata
2015
Research Centre for Cereal and Industrial Crops
2012-2015
Boston Children's Hospital
2013
There is evidence that nonalcoholic fatty liver disease (NAFLD) affected by gut microbiota. Therefore, we investigated its modifications in pediatric NAFLD patients using targeted metagenomics and metabolomics. Stools were collected from 61 consecutive diagnosed with (NAFL), steatohepatitis (NASH), or obesity 54 healthy controls (CTRLs), matched a case‐control fashion. Operational taxonomic units pyrosequenced targeting 16S ribosomal RNA volatile organic compounds determined solid‐phase...
Abstract Background Epidemiology of celiac disease (CD) is increasing. CD mainly presents in early childhood with small intestinal villous atrophy and signs malabsorption. Compared to healthy individuals, patients seemed be characterized by higher numbers Gram-negative bacteria lower Gram-positive bacteria. Results This study aimed at investigating the microbiota metabolome 19 children under gluten-free diet (treated disease, T-CD) 15 non-celiac (HC). PCR-denaturing gradient gel...
Beef chops were stored at 4°C under different conditions: in air (A), modified-atmosphere packaging (MAP), vacuum (V), or bacteriocin-activated antimicrobial (AV). After 0 to 45 days of storage, analyses performed determine loads spoilage microorganisms, microbial metabolites (by solid-phase microextraction [SPME]-gas chromatography [GC]-mass spectrometry [MS] and proton nuclear magnetic resonance [(1)H NMR]), diversity PCR-denaturing gradient gel electrophoresis [DGGE] pyrosequencing). The...
Abstract Background Despite the efficacy of immune checkpoint inhibitors (ICIs) only 20–30% treated patients present long term benefits. The metabolic changes occurring in gut microbiota metabolome are herein proposed as a factor potentially influencing response to immunotherapy. Methods metabolomic profiling was characterized 11 affected by non-small cell lung cancer (NSCLC) with nivolumab second-line treatment anti-PD-1 nivolumab. metabolomics analyses were performed GC–MS/SPME and 1 H-NMR...
Abstract Atopic dermatitis (AD) has been hypothesised to be associated with gut microbiota (GM) composition. We performed a comparative study of the GM profile 19 AD children and 18 healthy individuals aimed at identifying bacterial biomarkers disease. The effect probiotic intake ( Bifidobacterium breve plus Lactobacillus salivarius ) on modulation persistence in were also evaluated. Faecal samples analysed by real-time PCR 16S rRNA targeted metagenomics. Although probiotics, chosen for this...
To cite this article: Francavilla R, Calasso M, Calace L, Siragusa S, Ndagijimana Vernocchi P, Brunetti Mancino G, Tedeschi Guerzoni E, Indrio F, Laghi Miniello VL, Gobbetti De Angelis M. Effect of lactose on gut microbiota and metabolome infants with cow’s milk allergy. Pediatric Allergy Immunology 2012: 23 : 420–427. Abstract Allergic have an unusual gastrointestinal low numbers Bifidobacterium /Lactobacilli high levels Clostridium , staphylococci Escherichia coli. Hydrolyzed formula used...
Several studies in recent times have linked gut microbiome (GM) diversity to the pathogenesis of cancer and its role disease progression through immune response, inflammation metabolism modulation. This study focused on use network analysis weighted gene co-expression (WGCNA) identify biological interaction between ecosystem metabolites that could impact immunotherapy response non-small cell lung (NSCLC) patients undergoing second-line treatment with anti-PD1. Metabolomic data were merged...
Background Cystic fibrosis (CF) is a disorder affecting the respiratory, digestive, reproductive systems and sweat glands. This lethal hereditary disease has known or suspected links to dysbiosis gut microbiota. High-throughput meta-omics-based approaches may assist in unveiling this complex network of symbiosis modifications. Objectives The aim study was provide predictive functional model microbiota enterophenotype pediatric patients affected by CF under clinical stability. Methods...
The colonization and development of gut microbiota immediately after birth is highly variable depends on several factors, such as delivery mode modality feeding during the first months life. A cohort 31 mother neonate pairs, including 25 at-term caesarean (CS) 6 vaginally (V) delivered neonates (DNs), were included in this study 121 meconium/faecal samples collected at days 1 through 30 following birth. Operational taxonomic units (OTUs) assessed 69 stool by phylogenetic microarray HITChip...
Non-alcoholic fatty liver disease (NAFLD), considered the leading cause of chronic in children, can often progress from non-alcoholic (NAFL) to steatohepatitis (NASH). It is clear that obesity one main risk factors involved NAFLD pathogenesis, even if specific mechanisms have yet be elucidated. We investigated distribution intestinal bifidobacteria and lactobacilli stools four groups children: obese, obese with NAFL, NASH, healthy, age-matched controls (CTRLs).Sixty-one NAFL NASH children 54...
Abstract Background & Aim Sarcopenia is frequent in cirrhosis and associated with unfavourable outcomes. The role of the gut‐liver‐muscle axis this setting has been poorly investigated. aim study was to identify gut microbiota, metabolic inflammatory signatures sarcopenia cirrhotic patients. Methods Fifty patients assessed for presence by quantification muscle mass strength were compared age‐ sex‐matched controls. A multiomic analysis, including microbiota composition metabolomics, serum...
Background Ulcerative colitis (UC) is a multifactorial chronic inflammatory bowel disease (IBD) that affects the large intestine with superficial mucosal inflammation. A dysbiotic gut microbial profile has been associated UC. Our study aimed to characterize UC bacterial, fungal, and metabolic fingerprints by omic approaches. Methods The 16S rRNA- ITS2-based metataxonomics gas chromatography–mass spectrometry/solid phase microextraction (GC–MS/SPME) metabolomic analysis were performed on...
Abstract This work aimed at showing the effect of pheromone plantaricin A (PlnA) by Lactobacillus plantarum DC400 towards other sourdough lactic acid bacteria and potential PlnA to protect function human intestinal barrier. Growth survival were differently affected co‐cultivation with L. DC400. Compared mono‐cultures, sanfranciscensis DPPMA174 Pediococcus pentosaceus 2XA3 showed growth inhibition decreased viability when co‐cultured induced highest synthesis PlnA. Survival strain only...
Autism spectrum disorders (ASDs) is a multifactorial neurodevelopmental disorder. The communication between the gastrointestinal (GI) tract and central nervous system seems driven by gut microbiota (GM). Herein, we provide GM profiling, considering GI functional symptoms, neurological impairment, dietary habits. Forty-one 35 fecal samples collected from ASD neurotypical children (CTRLs), respectively, (age range, 3–15 years) were analyzed 16S targeted-metagenomics (the V3–V4 region)...