A5089095173- Antibiotic Resistance in Bacteria
- Reproductive tract infections research
- Gut microbiota and health
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 detection and testing
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Orthopedic Infections and Treatments
- Antimicrobial Peptides and Activities
- Heat shock proteins research
- Diet and metabolism studies
- Vector-borne infectious diseases
- Infections and bacterial resistance
- COVID-19 diagnosis using AI
- Plant Pathogenic Bacteria Studies
- Pancreatitis Pathology and Treatment
- Salmonella and Campylobacter epidemiology
- COVID-19 Clinical Research Studies
- Cervical Cancer and HPV Research
- Neonatal and Maternal Infections
- Viral Infections and Vectors
- Long-Term Effects of COVID-19
Sapienza University of Rome
2004-2022
Azienda Ospedaliera Sant'Andrea
2003-2021
CTO Andrea Alesini
2005-2021
Museum of Heilongjiang Province
2021
Bambino Gesù Children's Hospital
2013-2015
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2014
University of Chieti-Pescara
2008-2014
Boston Children's Hospital
2013
Istituto Superiore di Sanità
1998-2005
Vita-Salute San Raffaele University
2003
The colonization and development of gut microbiota immediately after birth is highly variable depends on several factors, such as delivery mode modality feeding during the first months life. A cohort 31 mother neonate pairs, including 25 at-term caesarean (CS) 6 vaginally (V) delivered neonates (DNs), were included in this study 121 meconium/faecal samples collected at days 1 through 30 following birth. Operational taxonomic units (OTUs) assessed 69 stool by phylogenetic microarray HITChip...
The presence and genetic content of integrons were investigated for 37 epidemiologically unrelated multiple-drug-resistant strains Salmonella enterica serotype Typhimurium from humans. All isolates resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, sulfonamides, trimethoprim, as well tetracycline and/or nalidixic acid; 20% them also gentamicin amikacin. Three different class 1 (In-t1, In-t2, In-t3) identified by Southern blot hybridization, PCR, DNA sequencing, these found...
Stenotrophomonas maltophilia has recently gained considerable attention as an important emerging pathogen in cystic fibrosis (CF) patients. However, the role of this microorganism pathophysiology CF lung disease remains largely unexplored. In present study for first time we assessed ability S. isolates to adhere and form biofilm experimental infection experiments using CF-derived bronchial epithelial IB3-1cell line. The flagella on adhesiveness IB3-1 cell monolayers was also by fliI mutant...
ObjectivesInfections caused by multidrug-resistant (MDR) Acinetobacter baumannii are a challenging problem worldwide. Here, the molecular epidemiology and genetic basis of antibiotic resistance in 111 MDR A. strains isolated from June 2005 to March 2009 infected patients 10 intensive care units (ICUs) central Italy were investigated.
ABSTRACT A conjugative IncL/M plasmid (pSEM) conferring resistance to gentamicin, amikacin, kanamycin, sulfonamides, and expanded-spectrum cephalosporins was found in pathogenic strains of Salmonella enterica serotype Typhimurium. Resistance aminoglycosides encoded by a sul1 -type class 1 integron (In-t3). An extended-spectrum beta-lactamase gene, bla SHV-5 , identified 3.5 kb downstream the integrase ( intI1 ) gene In-t3. Nucleotide sequence analysis 5.3-kb –In-t3 region pSEM highlighted...
Quantitation of neutrophil CD64 expression and procalcitonin (PCT) levels in blood samples have been recently proposed as useful tools for early detection sepsis. To determine the usefulness these tests, we analyzed 112 patients, admitted to an intensive care unit (ICU), presenting clinical symptoms sepsis, well 50 healthy controls. At end study, a retrospective analysis showed that only 52 ICU-patients presented real sepsis (positive culture). The results obtained indicated patients with 49...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination occurs through droplets and biological fluids released in the surroundings from patients or asymptomatic carriers. Surfaces objects contaminated by saliva nose secretions represent a risk for indirect transmission of disease 2019 (COVID-19). We assayed surfaces hospital living spaces to identify presence viral RNA spread fomites environment. Anthropic was monitored detecting microbiota signature using...
Heat shock proteins (HSPs) are a family of with immunogenic and proinflammatory properties. Human Chlamydia pneumoniae (Cp) HSP60 were found in patients stable coronary disease.We measured the levels anti-Cp-HSP60 anti-Cp immunoglobulin G (IgG) 179 unstable angina, 40 acute myocardial infarction, angina (SA), as well 100 control subjects. Forty-one syndromes (ACS) also studied at follow-up. We plasma high-sensitivity C-reactive protein (hs-CRP) troponin T (TnT). Seropositivity to Cp-HSP60...
Stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen which currently isolated with increasing frequency from the airways of cystic fibrosis (CF) patients. In this study 13 S. strains (11 independent CF patients, and two non-CF respiratory reference strains) have been characterized for expression several virulence-associated factors. particular, ability to form biofilm on abiotic surfaces has determined correlated different features, such as motility, adherence invade...
Proper protein localization is critical for bacterial virulence. PhoN2 a virulence-associated ATP-diphosphohydrolase (apyrase) involved in IcsA-mediated actin-based motility of S. flexneri. Herein, by analyzing ΔphoN2 mutant the flexneri strain M90T and generating phoN2::HA fusions, we show that PhoN2, periplasmic strictly localizes at poles, with strong preference old pole, pole where IcsA exposed, it required proper exposition. PhoN2-HA was found to be polarly localized both when...
ABSTRACT Evidence linking Chlamydia pneumoniae infection to atherosclerosis and atherothrombotic events has recently emerged. A primary candidate implicated in these pathogenetic is the 60-kDa chlamydial heat shock protein (HSP60). Another putative activate a potential proinflammatory mechanism outer membrane 2 (OMP2). We have generated both HSP60 OMP2 recombinant antigens nondenatured form shown that (i) two were highly immunogenic mice (ii) murine antisera thus recognized native C....
Abstract Background Low T3 syndrome is frequent in patients admitted to intensive care units for critical illness and pneumonia. It has been reported also with COVID-19, Hodgkin disease chronic lymphocytic leukemia. We analyzed the clinical relevance of COVID-19 and, particular, those associated hematological malignancies. Methods Sixty-two consecutive patients, hospitalized during first wave SARS-CoV-2 outbreak Sant’Andrea University Hospital Rome, were subdivided 38 (Group A), showing low...
The role in virulence of the Shigella flexneri ospB-phoN2 operon has been evaluated. Here we confirm that OspB is an effector and show apyrase, product phoN2, may be a factor, since it required for efficient intercellular spreading. Apyrase important deoxynucleoside triphosphate-hydrolyzing activity-independent manner, suggesting act as interaction partner process IcsA localization.
In September 1997, a 25-year-old Italian woman with cystic fibrosis (CF) spent 3 weeks in Thailand. August 1998, her pulmonary function rapidly declined, productive cough and intermittent fever. Chest x-ray films revealed diffuse, small, patchy opacities the upper lobes. Burkholderia pseudomallei (BP) was isolated from specimens of patient's sputum identified by means 16S rDNA sequencing. The diagnosis melioidosis serologically confirmed. Continuous therapy ceftazidime co-trimoxazole...
Lactobacilli are Gram-positive rod-shaped bacteria that inhabit the oral cavity, gastrointestinal tract, vagina and nasal cavity. In this report, a rare case of Lactobacillus jensenii endocarditis in 47-year-old immunocompetent patient is described. Blood cultures replaced mitral valve were positive for L. as assessed by 16S rRNA gene sequencing. Based on susceptibility tests was successfully treated with mixture teicoplanin meropenem antimicrobial therapy.