A5043935773- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Toxoplasma gondii Research Studies
- Coccidia and coccidiosis research
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Parasites and Host Interactions
- Bacteriophages and microbial interactions
- Parasitic Diseases Research and Treatment
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Parasite Biology and Host Interactions
- Parasitic infections in humans and animals
- Glycosylation and Glycoproteins Research
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Insect behavior and control techniques
- Food Allergy and Anaphylaxis Research
- Solid-state spectroscopy and crystallography
- HIV/AIDS drug development and treatment
- Clostridium difficile and Clostridium perfringens research
- Studies on Chitinases and Chitosanases
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
Istituto Superiore di Sanità
2007-2024
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2012
Sapienza University of Rome
1989-1998
ABSTRACT Sequencing of DNA from 15 expanded-spectrum cephalosporin (e.g., ceftriaxone)-resistant Salmonella isolates obtained in the United States revealed that resistance to ceftriaxone all was mediated by cmy-2. Hybridization patterns three plasmid structures containing cmy-2 these isolates. These data suggest spread among strains is occurring through mobilization gene into different backbones and consequent horizontal transfer conjugation.
The presence and genetic content of integrons were investigated for 37 epidemiologically unrelated multiple-drug-resistant strains Salmonella enterica serotype Typhimurium from humans. All isolates resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, sulfonamides, trimethoprim, as well tetracycline and/or nalidixic acid; 20% them also gentamicin amikacin. Three different class 1 (In-t1, In-t2, In-t3) identified by Southern blot hybridization, PCR, DNA sequencing, these found...
Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden disease is particularly high among children living developing areas the world, where transmission favored by lower hygienic standards and scarce availability safe water. However, asymptomatic infection polyparasitism are also commonly observed poor settings. Here, we investigated prevalence intestinal two small fishing villages, Porto Said (PS) Santa Maria da Serra (SM), situated along river...
ABSTRACT Multidrug-resistant Vibrio cholerae O1 El Tor strains isolated during the 1994 outbreak of cholera in Albania and Italy were characterized for molecular basis antibiotic resistance. All found to be resistant tetracycline, streptomycin, spectinomycin, trimethoprim, sulfathiazole, vibriostatic compound O/129 (2,4-diamino-6,7-diisopropylteridine). Resistance genes self-transferable by a conjugative plasmid about 60 MDa, with exception spectinomycin resistance, which was conferred aadA1...
ABSTRACT A conjugative IncL/M plasmid (pSEM) conferring resistance to gentamicin, amikacin, kanamycin, sulfonamides, and expanded-spectrum cephalosporins was found in pathogenic strains of Salmonella enterica serotype Typhimurium. Resistance aminoglycosides encoded by a sul1 -type class 1 integron (In-t3). An extended-spectrum beta-lactamase gene, bla SHV-5 , identified 3.5 kb downstream the integrase ( intI1 ) gene In-t3. Nucleotide sequence analysis 5.3-kb –In-t3 region pSEM highlighted...
ABSTRACT We studied apoptosis in a human ileocecal adenocarcinoma tumor cell line (HCT-8) infected with Cryptosporidium parvum , from 2 to 72 h postinfection (h.p.i.). At h.p.i., the percentage of annexin V-positive cells culture had increased 10% compared 2.5% noninfected control culture; sorted expressed mRNA FasL, active form caspase 3, and high 3 activity, whereas neighboring same showed no signs apoptosis. 24 percentages early (annexin V positive) late (DNA fragment) apoptotic were 13...
Cryptosporidiosis, caused by coccidian parasites of the genus Cryptosporidium, is a major cause human gastrointestinal infections and poses significant health risk especially to immunocompromised patients. Despite intensive efforts for more than 20 years, there currently no effective drug treatment against these protozoa. This study examined zoonotic species Cryptosporidium parvum at two important stages its life cycle: non-excysted (transmissive) excysted (infective) forms. To increase our...
The flagellated protozoan Giardia duodenalis is a common gastrointestinal parasite of mammals, including humans. Molecular characterizations have shown the existence eight genetic groups (or assemblages) in G. species complex. Human infections are caused by assemblages A and B, which infect other mammals as well. Whether transmission routes, animal reservoirs associations with specific symptoms differ for assemblage B not clear. Furthermore, occurrence clinical significance mixed (A+B) also...
Dientamoeba fragilis is a common intestinal parasite in humans. Transmission routes and natural host range are unknown. To determine whether pigs hosts, we analyzed 152 fecal samples by microscopy molecular methods. We confirmed that harbor genotypes found humans, suggesting zoonotic potential.
Background and Aim. Trichinellosis, caused by nematodes of the genus Trichinella, is a zooanthroponotic infection which importance steadily increasing due to its widespread distribution significant impact on human animal health. This study focuses species Trichinella nativa, one most resistant adapted cold conditions, frequently infects various wild mammalian species. Materials Methods. Recent molecular studies have helped clarify genetic structure T. but gaps remain in understanding...
Two class 1 integrons, In-t1 and In-t2, were previously identified in IncFI plasmids of Salmonella enterica serotype Typhimurium. Molecular analysis revealed a close physical link between the two integrons. is preceded by transposase genes Tn21, whereas In-t2 located downstream 3'-conserved segment (3'-CS) In-t1, head-to-tail configuration. shows peculiar sequence 3'-CS, containing an extended version open reading frame known as ORF341 (referred to ORF341E) novel trimethoprim resistance...
ABSTRACT T-cell-mediated immunity plays a central role in the host response to Cryptosporidium parvum . Human T-cell clones (TCC) were isolated from peripheral blood mononuclear cells of five healthy donors with prior cryptosporidiosis by use C. crude extract, two antigen fractions obtained ion-exchange chromatography (IEC1 and IEC2), recombinant peptides (SA35 SA40) sporozoites. The lines derived one recently infected donor had higher proportion (26 38%) T exhibiting γ/δ receptor (γ/δ-TCR)...
Wound myiasis is the infestation of open wounds mammalian hosts caused by larvae various species flies. This kind can be a serious problem for immobilized patients with wounds. Here, we identify dipteran larva found in tracheostomy wound child affected severe spinal muscular atrophy. The collected was dissected and microscopically analyzed. DNA extracted from part used molecular identification. A 487 bp fragment, including 5.8 S, internal transcribed spacer 2 (ITS2), 28S, amplified using...
Antibodies targeting Receptor Binding Domain (RBD) of SARS-CoV-2 have been suggested to account for the majority neutralizing activity in COVID-19 convalescent sera and several antibodies (nAbs) isolated, characterized proposed as emergency therapeutics form monoclonal (mAbs). However, variants are rapidly spreading worldwide from sites initial identification. The concern (VOC) B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) B.1.167.2 (Delta) showed mutations spike protein potentially able...
is a common cause of zoonotic disease and main diarrhea in newborns. Effective drugs or vaccines are still lacking. Oocyst the infective form parasite; after its ingestion, oocyst excysts releases four sporozoites into host intestine that rapidly attack enterocytes. The membrane protein CpRom1 large rhomboid protease expressed by recognized as antigen immune system. In this study, we observed release with extracellular vesicles (EVs) was not previously described. To investigate phenomenon,...
In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study children from low-income communities Sao Paulo State, Brazil. Fecal samples daycare center attendees up 6 years old (n=156) staff members (n=18) were submitted PCR sequencing D. as well microscopic examination for presence other intestinal parasites. All assessed asymptomatic 10.3% (16/156) positive fragilis. No worker was found be positive. An...