A5066361794- Virology and Viral Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- Respiratory viral infections research
- Immune responses and vaccinations
- Essential Oils and Antimicrobial Activity
- Immunodeficiency and Autoimmune Disorders
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Syphilis Diagnosis and Treatment
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Microbial bioremediation and biosurfactants
- COVID-19 Clinical Research Studies
- Genetic factors in colorectal cancer
- Inflammatory mediators and NSAID effects
- Rabies epidemiology and control
- Microbial infections and disease research
- COVID-19 epidemiological studies
- Multicomponent Synthesis of Heterocycles
- Computational Drug Discovery Methods
- Osteomyelitis and Bone Disorders Research
Istituto Superiore di Sanità
2015-2025
Natural products are a rich source of bioactive molecules that have potential pharmacotherapeutic applications. In this study, we focused on Artemisia annua (A. annua) and its enriched extracts which were biologically evaluated in vitro as virucidal, antiviral, antioxidant agents, with application against the COVID-19 infection. The crude extract showed antiviral effects concentrations did not affect cell viability. Scopoletin, arteannuin B artemisinic acid (single fractions isolated from A....
West Nile virus (WNV) is a vector-borne disease responsible for causing epidemics in many areas of the world. The maintained nature by an enzootic bird-mosquito-bird cycle and occasionally transmitted to other hosts, such as equines humans. Culex species, particular ubiquitous species pipiens thought play major vector role both epizootic maintenance transmission WNV. Introduced Europe recent years, since 2008 WNV has been stably circulating mainly Northeastern regions Italy, although...
ObjectivesTo evaluate whether the increase of temperature can influence environmental endurance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).MethodsVirus was inoculated on a plastic surface and harvested at predefined time-points in parallel 20°C–25°C (room temperature; RT) 28°C (June JT). Samples were tested by TCID50 titres Vero cells.ResultsOur results confirm that fomite transmission emerging SARS-CoV-2 is possible: virus reserved its ability to infect cells for up 84...
The planetary crisis regarding water resources means that new methods are needed to monitor large areas of basins threatened by chemical and natural pollutants, together with climate change. With the aim detect oil spill, we applied an algorithm, which could consistently reliably confirm presence in five polluted lake waters analyzed. Combined algorithm application metagenomic analysis from spill had been detected satellite identified drivers microbial response pollution. Based on ortholog...
Since late 2023, the Metropolitan City of Milan and surrounding areas (northern Italy) have been experiencing a resurgence measles, with most cases detected starting from January 2024. During this brief period, we observed measles in travellers endemic areas, participants international events, vaccinees healthcare workers. Indigenous also identified. Even though not yet identified large disruptive outbreaks, strengthening surveillance vaccination activities is pivotal to help limit impact spread.
Thirty pools from 900 (540 females and 360 males) Phlebotomus perfiliewi sandflies collected during the summer of 2012 in Fermo area (Marche Region, central Italy) were tested for presence Phleboviruses. A nested polymerase chain reaction was performed using degenerated primers amplifying a fragment gene (large segment) nucleoprotein (small genus Phlebovirus. One pool positive Toscana virus, as expected results studies area, six putative novel Virus isolation Vero cells performed. Minimum...
The COVID-19 pandemic represents an unprecedented global emergency. Despite all efforts, remains a threat to public health, due the complexity of mass vaccination programs, lack effective drugs, and emergence new variants. A link has recently been found between risk developing severe infection high level oxidative stress. In this context, we have focused our attention on natural compounds with aim finding molecules capable acting through dual virucidal-antioxidant mechanism. particular,...
Subacute sclerosing panencephalitis (SSPE) is a late complication of measles virus infection that occurs in previously healthy children. This disease has no specific cure and associated with high degree disability mortality. In recent years, there been an increase its incidence relation to reduction vaccination adherence, accentuated by the COVID-19 pandemic. this article, we take stock current evidence on SSPE report our personal clinical experience. We emphasise that, date, only effective...
An outbreak of measles virus genotype B3 is ongoing in Milan and surrounding areas since February 2025, with 27 cases identified 32 laboratory-confirmed cases. Most were locally acquired young adults. Phylogenetic analysis indicated the presence a unique lineage closely related to strains circulating Morocco. The lack epidemiological links between several affected individuals suggests case numbers are being underestimated. continuous transmission raises concerns about potential...
Abstract West Nile virus ( WNV ) is a zoonotic arboviral pathogen transmitted by mosquitoes in cycle that involves wild birds as reservoir hosts. The responsible for outbreaks of viral encephalitis humans and horses. In Europe, Culex pipiens (Diptera: Culicidae) considered to be the main vector , but other species such Stegomyia albopicta (= Aedes albopictus may also act competent vectors this virus. Since 2008 human cases disease have been reported northeast Italy. 2011, new areas southern...
The emergence of the new pathogen SARS-CoV-2 determined a rapid need for monoclonal antibodies (mAbs) to detect virus in biological fluids as tool identify infected individuals be treated or quarantined. majority commercially available antigenic tests rely on detection N antigen biologic fluid using anti-N antibodies, and their capacity specifically subjects by is questionable due several structural analogies among proteins different coronaviruses. In order produce specific BALB/c mice were...
In 2017, Italy experienced one of the largest outbreaks measles in recent years, with 5404 notified cases and 4347 confirmed cases. A further 2029 were during first 6 months 2018, 1516 them laboratory-confirmed. The B3 D8 genotypes identified as those responsible for outbreak. Possible transmission routes can be established by monitoring circulating virus strains support national health authorities to warn people travellers.
The oil-water interface formed during an oil spill represents a challenging environment for pelagic communities living in aquatic ecosystems. At this anoxic barrier, we report the formation of microbial hydrocarbonoclastic biofilm capable electron transfer along water column. This generated membrane surface-active compounds that allowed spontaneous separation electrical charges, causing establishment anodic and cathodic region and, as result, creation liquid fuel cell. Such floating was...
During recent years, various mumps outbreaks have occurred among populations vaccinated for worldwide. In Italy, improving routine coverage with two doses of measles, and rubella (MMR) vaccine is one the key strategies to eliminate measles rubella. To monitor effect vaccination programme on population, surveillance these vaccine-preventable diseases has been implemented. This provided opportunity evaluate accuracy clinical diagnosis those diseases, including mumps. fact, children may develop...
Antibodies targeting Receptor Binding Domain (RBD) of SARS-CoV-2 have been suggested to account for the majority neutralizing activity in COVID-19 convalescent sera and several antibodies (nAbs) isolated, characterized proposed as emergency therapeutics form monoclonal (mAbs). However, variants are rapidly spreading worldwide from sites initial identification. The concern (VOC) B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) B.1.167.2 (Delta) showed mutations spike protein potentially able...
The current SARS-CoV-2 pandemic and the likelihood that new coronavirus strains will emerge in immediate future point out urgent need to identify pan-coronavirus inhibitors. Strigolactones (SLs) are a class of plant hormones with multifaceted activities whose roles plant-related fields have been extensively explored. Recently, we proved SLs also exert antiviral activity toward herpesviruses, such as human cytomegalovirus (HCMV). Here show synthetic TH-EGO EDOT-EGO impair β-coronavirus...
COVID-19 continues to claim victims in the world, especially among elderly subjects and people suffering from chronic-degenerative pathologies, like cardiovascular diseases. Several vaccines drugs have been developed mitigate infection spread its deleterious consequences. However, emergence of new variants requires identification solutions deal with challenging mutations. In this context, investigation phytocomplexes related compounds used folk medicine culinary purposes may lead unfold...
The neurologic sequelae post-measles are less common than other complications measles-related and can lead to severe disability or death: primary measles encephalitis (PME), acute post-infectious encephalomyelitis (APME), inclusion body (MIBE), subacute sclerosing panencephalitis (SSPE). SSPE syndrome affect people years from the virus infection, as result of persistence defective viral particles in brain cells. Clinical onset typically manifests with progressive intellectual deterioration,...
The European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to halt indigenous transmission measles in its 53 Member States by 2015. In view goal elimination, it is great importance assess circulation wild-type virus (MV). Genetic analysis indispensable understand epidemiology measles. Urine and saliva samples were collected between May 2002 December 2007, order find origins routes wild type circulation. RT-PCR was performed on total 414 clinical...