A5075272941- Cancer-related molecular mechanisms research
- Influenza Virus Research Studies
- Ginseng Biological Effects and Applications
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- interferon and immune responses
- Ovarian cancer diagnosis and treatment
- Identification and Quantification in Food
- Enzyme Structure and Function
- Pharmacological Effects of Natural Compounds
- Carcinogens and Genotoxicity Assessment
- PARP inhibition in cancer therapy
- Viral Infections and Immunology Research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Kruppel-like factors research
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Mitochondrial Function and Pathology
- Cytomegalovirus and herpesvirus research
- Genomics, phytochemicals, and oxidative stress
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Ocular Oncology and Treatments
Jilin University
2007-2025
Dana-Farber Cancer Institute
2015
For solid tumors, hypoxia is associated with disease aggressiveness and poor outcomes. In addition to undergoing broad intracellular molecular metabolic adaptations, hypoxic tumor cells extensively communicate their microenvironments facilitate conditions favorable for survival, growth, metastasis. This communication mediated by diverse secretory factors, including exosomes (extracellular vesicles of endosomal origin). Exosomal cargo altered considerably hypoxia, significant impacts on...
<b><i>Background:</i></b> Colorectal cancer is a common malignant tumor of the digestive tract, morbidity rate which rising in recent years. Ginsenoside Rh3 was reported to have anticancer activity; however, underlying mechanism still needs be explored depth. <b><i>Methods:</i></b> Rabbit blood used test hemolytic effects ginsenoside protopanaxadiol (PPD), Rh2, Rh3, and Rg3. Human colorectal SW1116 cells were treated with different...
The mechanism of ginsenoside Rh3 activity against cancer remains unclear. This study aimed to investigate the underlying mechanism. effects on cell proliferation, migration and invasion, cycle apoptosis were analyzed using CCK-8 assay, transwell assay flow cytometry, respectively. RNA transcriptome was sequenced data by R software. Protein expression protein-protein interactions determined Western blotting co-immunoprecipitation, results showed inhibited HCT116 migration, arrested cells at...
This study explored the role of lymphocyte antigen 6 family member D (LY6D) in colon cancer stem cells’ (CCSCs) proliferation and invasion. LY6D was knocked down using siRNA, down-regulation verified Western blotting. After knockdown, CCSCs’ proliferation, stemness, invasion were suppressed, whereas apoptosis increased. Gene Ontology (GO) enrichment analysis revealed that differentially expressed genes (DEGs) between siLY6D negative control groups significantly enriched cell–substrate...
Lung cancer seriously threatens human health. To explore the molecular mechanism of 20(S)-Protopanaxadiol (PPD) on non-small cell lung cells, we investigated transcriptional profile PPD-treated NCI-H1299 cells. Cell proliferation, cycle, and apoptosis were detected using counting kit-8 flow cytometry, respectively. Differentially expressed genes (DEGs) between untreated cells determined RNA sequencing bioinformatic analysis. Protein phosphorylation was Western blotting. Data mRNA expression...
Coxsackie B3 virus (CVB3) is the most significant pathogen causing myocarditis in humans, and antiviral therapy would be effective early stages of disease. Here we provide evidence that BW001, a C-type CpG oligodeoxynucleotide, induces anti-CVB3 activity human peripheral blood mononuclear cells (PBMCs). In parallel, have demonstrated BW001 PBMCs to express mRNAs multiple types interferon (IFN), including IFN-alpha, IFN-beta, IFN-omega IFN-gamma, at least 11 subtypes IFN-alpha. The induced...
To uncover the role of microRNAs in occurrence and development uveal melanoma (UM), we used R language packages this study to analyze correlations between expression microRNA isoforms, their target genes, clinical data for UM patients retrieved from The Cancer Genome Atlas (TCGA). We Weighted Correlation Network Analysis (WGCNA) divide profiles different into 10 modules, among which blue yellow modules were associated with survival. Hsa-miR-513a-5p, miR-506-3p, miR-508-3p, miR-140-3p,...
GFI1 is a DNA binding transcriptional repressor, it shown to be an important gene associated with blood cells development and many diseases (Möröy et al., 2015). But the role of in human hematopoieticdevelopment has not been known (Thambyrajah 2016). To illustrate function hematopoieticdevelopment, we constructed GFI1-2 × flag-tag knock-in embryonic stem cell line by CRISPR/Cas9 mediated targeting, would effective tool study GFI1. The could express can identified western blot...
Abstract Background High mobility group box (HMGB) family protein Ixr1 has been shown to be involved in DNA damage repair, however, its role and mechanism remain largely unclear. Methods Genes of S. cerevisiae were deleted or tagged with myc, GFP, mcherry using the lithium acetate method. Sensitivity strains hydroxyurea (HU), methyl methanesulfonate (MMS), camptothe-cin (CPT), 4-nitroquinoline N-oxide (4-NQ), Zeocin was tested. Distribution GFP fusion proteins visualized laser scanning...