Serologic tests have been widely used for the diagnosis of asymptomatic visceral leishmaniasis. This study evaluated five serologic infection: enzyme-linked immunosorbent assay (ELISA) using promastigote antigen (ELISAp), ELISA recombinant K39 (ELISA rK39), and K26 rK26) antigens, an indirect immunofluorescence test Leishmania (Leishmania) amazonensis (IIFT), immunochromatographic rK39 (TRALd). As a reference regarding performance tests, patients with classic leishmaniasis originating from...

The clinical manifestations of sickle cell disease are related to the polymerization hemoglobin S. chronic hemolysis caused by this condition often causes formation gallstones that can migrate and block common bile duct leading acute abdomen. This study aimed evaluate profile patients with cholelithiasis. Patients were separated into groups according presence or absence Socioepidemiological characteristics, such as gender, age, use hydroxyurea other hemoglobinopathies researched in medical...

A alta morbimortalidade referida na doença falciforme (DF) levou-nos a estudar o perfil epidemiológico e respectivas intercorrências clínicas dos pacientes atendidos no Hemocentro Regional (HR) Hospital de Clínicas da Universidade (HC-U), 1998 2007. Estudo retrospectivo 151 pacientes, avaliados quanto a: idade, gênero, cor pele, procedência, diagnóstico, causa atendimento HR, tempo internação HC-U idade em caso óbito. Foi realizada análise estatística descritiva simples. média foi 17,7 anos,...

BACKGROUND: Allogeneic blood transfusions are the second most frequent route of Chagas' disease transmission in countries where is endemic. The prevention transfusion‐associated has been attempted through clinical and serologic screening donors and/or by addition trypanomicidal substances such as gentian violet (GV) to stored for 24 hours. present study describes an alternative method chemoprophylaxis that reduces sterilization time using a combination low‐concentration GV, ascorbic acid...

BACKGROUND : Transfusion‐associated Chagas' disease (TA‐CD) is a worldwide problem. Measures adopted to prevent TA‐CD include the clinical and serologic screening of blood donors and/or inactivation Trypanosoma cruzi present in collected blood, using gentian violet as trypanocidal agent. This study investigated efficacy white cell‐reduction filters removing T. from infected blood. STUDY DESIGN AND METHODS Human was contaminated with 2 or 150 parasites per mL then left unfiltered filtered...

To identify the occurrence and causes of platelet refractoriness in oncohematologic patients. Platelet (unsatisfactory post-transfusion increment) is a severe problem that impairs treatment patients not routinely investigated most Brazilian services. Forty-four episodes concentrate transfusion were evaluated 16 according to following parameters: corrected count increment, clinical conditions detection anti-platelet antibodies by immunofluorescence test (PIFT) panel reactive against human...

A alta prevalência de doadores chagásicos nos bancos sangue do Brasil (6,9%) e da América Latina (6,5%), nas décadas 60 70, aliada ao combate vetor a partir dos anos setenta, fez com que doença Chagas transfusional, década oitenta, se tornasse o principal mecanismo transmissão na maioria países endêmicos. Contudo, os resultados altamente favoráveis cobertura sorológica doadores, reduziu soropositividade para 0,2% 1,3%, respectivamente, no índice anual, via transfusão Brasil, 20.000 13 em...

Epidemiological screening combined with serological tests has become an important tool at blood banks for the characterization of donors or without Trypanosoma cruzi infection. Thus, objective present study was to describe sociodemographic and epidemiological characteristics non-negative serology T. cruzito determine possible risk factors associated ineligibility. Sociodemographic data were collected by analysis patient histories interviews. The analyzed descriptively using absolute relative...

The large diversity of red blood cell antigens favors, especially in multi-transfused patients, the occurrence autoimmunization and alloimmunization with risk hemolytic transfusion reactions. Thus, this study aimed to determine rates these individuals, as well types alloantibodies their systems, clinical epidemiological aspects frequency autoimmunity alloimmunized non-alloimmunized patients.In a retrospective study, 153 patients from 2006 2014 were evaluated. Sixty-eight had...

The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there no perfect gold standard. We evaluated an enzyme immunoassay (EIA), indirect hemaglutination (IHA), and immunofluorescence (IIF) test for detecting Cruzi antibodies in Brazilian donors. results were submitted latent class analysis, a radioimmunopreciptation (RIPA) was performed on repeatedly positive samples. Among 1951...

Atualmente, um dos maiores problemas na triagem sorológica de doadores sangue para doença Chagas é a alta freqüência reações indeterminadas, o que faz com muitos indivíduos sadios sejam rotulados como portadores uma grave. O presente trabalho tem objetivo analisar comportamento sorológico do Hemocentro Regional Uberaba, MG e propor mecanismos reduzir índice inaptidão essa doença. Através estudo retrospectivo, foi analisado resultado 79.729 amostras obtidas doações realizadas neste Serviço...

INTRODUCTION: A retrospective study was conducted to assess the occurrence of blood donations that were ineligible due Chagas disease infection from 1995 2009 at Uberaba Regional Blood Center (HRU), Brazil, verify tendency this ineligibility, and describe epidemiologic profile donors. METHODS: Retrospective studies serological ineligibility disease, statistical analysis by means chi-square test odds ratio, tendencies using a dispersion graph linear correlation coefficient (r) performed....

Irradiation of blood components with ionizing radiation generated by a specific device is recommended to prevent transfusion-associated graft-versus-host disease. However, linear accelerator can also be used in the absence such device, which case bank facility studied herein. In order evaluate quality irradiated packed red cells, this study aimed determine whether procedure currently employed effective inhibiting proliferation T lymphocytes without damaging components. The lymphocytes,...

We propose a new general Bayesian latent class model for evaluation of the performance multiple diagnostic tests in situations which no gold standard test exists based on computationally intensive approach. The modeling represents an interesting and suitable alternative to models with complex structures that involve case several conditionally independent tests, covariates, strata different disease prevalences. technique stratifying population according prevalence rates does not add further...

Saprophytic fungi are being increasingly recognized as etiologic agents of mycoses in immunosuppressed patients. We report a case subcutaneous infiltration by Aureobasidium pullulans, likely due to traumatic inoculation, neutropenic patient during chemotherapy for chronic lymphocytic leukemia. The was treated with amphotericin B deoxycholate but subsequently switched itraconazole, which improved the lesion. This highlights importance considering unusual fungal infections critically ill...

SUMMARY Objectives The aim of this study is to keep a record regular human platelet antigens ( HPA )‐typed blood donors and compare their allele frequencies with those reported in other populations. Background are polymorphisms expressed on membrane glycoproteins. They can generate an immune response leading alloimmunisation that may show clinical manifestations, such as neonatal alloimmune thrombocytopenia, post‐transfusion purpura refractoriness. Platelet not uncommon, therefore, for...

The aim of the present study was to outline serological profile hepatitis C among blood donors seen at Uberaba Regional Blood Center, Hemominas Foundation, over last 14 years. frequency compared between first-time and repeat epidemiological characteristics those with positive indeterminate ELISA anti-HCV (third fourth generation) were analyzed based on donor histories kept in archives Center. ineligibility rate 0.3%, higher prevalence group donors. We did not find any significant differences...

Abstract Objective Investigate the gonadal hormonal function in sickle cell individuals. Context Sickle disease (SCD) is associated with delayed physical and sexual development, it has been related to both primary testicular failure hypothalamo‐pituitary‐gonadal axis abnormalities. Design The study of pituitary gonadotrophin reserve was done evaluating levels before after stimulation by gonadoliberin. Patients Male patients homozygous SCD (18‐39 years, median = 29.5 years). Measurements...