A5044217734- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Nanofabrication and Lithography Techniques
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Antenna Design and Analysis
- Advanced MEMS and NEMS Technologies
- Electrochemical sensors and biosensors
- Peptidase Inhibition and Analysis
- Molecular Junctions and Nanostructures
- Advanced Antenna and Metasurface Technologies
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Protease and Inhibitor Mechanisms
- Polymer Surface Interaction Studies
- RFID technology advancements
- Electrowetting and Microfluidic Technologies
- Advanced Chemical Sensor Technologies
- Glycosylation and Glycoproteins Research
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
Sookmyung Women's University
2016-2025
Korea Institute of Machinery and Materials
2022
University of California, Davis
2011-2017
Government of the Republic of Korea
2017
Seoul Institute
2017
Korea Post
2012-2014
Pohang University of Science and Technology
2012-2014
University of Ulsan
2012-2014
University of California System
2014
Seoul National University
2003-2013
Monitoring activity of single cells has high significance for basic science and diagnostic applications.
In the liver, hepatocytes are exposed to a large array of stimuli that shape hepatic phenotype. This in vivo microenvironment is lost when cultured standard cell cultureware, making it challenging maintain hepatocyte function vitro. Our article focused on one least studied inducers phenotype-the mechanical properties underlying substrate. Gel layers comprised thiolated heparin (Hep-SH) and diacrylated poly(ethylene glycol) (PEG-DA) were formed glass substrates via radical mediated thiol-ene...
Abstract Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T‐cells from heterogeneous cell suspension then release desired cells groups of by UV‐induced photodegradation. Flow cytometry analysis the retrieved revealed approximately 95 % purity T‐cells, suggesting that this substrate had excellent specificity. To demonstrate possibility sorting according their function,...
Diagnosis of coronavirus disease (COVID-19) is important because the emergence and global spread severe acute respiratory syndrome 2 (SARS-CoV-2). Real-time polymerase chain reaction (PCR) widely used to diagnose COVID-19, but it time-consuming requires sending samples test centers. Thus, need detect antigens for rapid on-site diagnosis rather than PCR increasing. We quantified nucleocapsid (N) protein in SARS-CoV-2 using an electro-immunosorbent assay (El-ISA) a multichannel impedance...
Matrix metalloproteinases (MMPs) regulate composition of the extracellular matrix and play a critical role in cancer, fibrosis, wound healing. This article describes novel peptide-based electrochemical biosensor for detecting activity cell-secreted protease MMP9. In this sensing strategy, peptide specific to MMP9 was modified with redox label (methylene blue (MB)) immobilized on microfabricated 300 μm diameter Au electrodes. Challenging electrodes known concentrations resulted cleavage MB...
The origin of the fluorescence polymerized dopamine derivatives was first clarified and fluorescent oligomeric (F-ODA) could detect Fe<sup>2+</sup> ions with very high specificity.
The objective of this research is to investigate Fenton and persulfate oxidation with zero-valent iron [Fe(0)] as a batch type ex-situ remediation technology for the treatment diesel-contaminated soil. Results from experiments indicate that Fe(0) better catalyst H2O2 than Fe2+ enhancement in system. Maximum removal was obtained after 12 h when 1 2 g were added hydrogen peroxide (250 mg/L) mg/L), respectively, soil-water As amounts increased three times at optimal ratio, total petroleum...
Abstract The biomaterial scaffolds for regenerative medicine need to be rationally designed achieve the desired cell fate and function. This paper describes development of hydrogel microstructures cultivation primary hepatocytes. Four different micropatterned surfaces are tested: 1) poly(ethyelene glycol) (PEG) microwells patterned on glass, 2) heparin 3) PEG hydrogel‐coated substrates, 4) substrates. latter constructed by a combination micromolding microcontact printing techniques create...
Matrix metalloproteinases (MMPs) play a central role in the breakdown of extracellular matrix and are typically upregulated cancer cells. The goal present study is to develop microwells suitable for capture cells detection cell-secreted proteases. Hydrogel comprised poly(ethylene glycol) (PEG) were photopatterned on glass modified with ligands promote cell adhesion. To sense protease release, peptides cleavable by MMP9 designed contain donor/acceptor FRET pair (FITC DABCYL). These sensing...
A method for capture and release of cells was developed using a photolabile linker antibody-attached glass surface with poly(ethylene glycol) (PEG)-pattern.
Conducting polymer hydrogel is fabricated atop gold or ITO electrodes and functionalized with monoclonal antibodies. Binding of interferon-γ molecules causes redox properties conductive to change in a concentration-dependent fashion without the need for washing sample handling steps. This remains functional fouling media such as whole blood. As service our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized...
Abstract BACKGROUND This study investigated the oxidation of spent caustic, strong alkaline liquid waste from refinery and petrochemical plants, by hydrogen oxide persulfate activated with zero‐valent iron (Fe(0)) as an alternative treatment process. Using batch experiments, mineralization caustic was evaluated monitoring total organic carbon (TOC). RESULTS TOC removal not successfully achieved peroxide or only; mineralized when Fe(0) added. With 1 g Fe(0), 25 35% initial reduced in presence...
We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering surface could be degraded by UV exposure release specific interest.
Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore, these can be used diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-γ from T-cells is for blood-based diagnosis tuberculosis (TB). Our lab has previously developed an atpamer-based electrochemical biosensor rapid and sensitive detection IFN-γ. In this study, we explored the use silicon nanowires (NWs) as way create nanostructured electrodes with enhanced...