A5088654770- Nanoparticle-Based Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Geological and Geochemical Analysis
- RNA Interference and Gene Delivery
- Medical Imaging and Pathology Studies
- Atrial Fibrillation Management and Outcomes
- Neonatal Respiratory Health Research
- Geochemistry and Geologic Mapping
- MicroRNA in disease regulation
- Aortic Disease and Treatment Approaches
- Biosensors and Analytical Detection
- Pancreatic function and diabetes
- Microfluidic and Bio-sensing Technologies
- Adipose Tissue and Metabolism
- Geochemistry and Geochronology of Asian Mineral Deposits
- Cancer Research and Treatments
- Diabetes and associated disorders
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Lanthanide and Transition Metal Complexes
- Advanced Biosensing Techniques and Applications
Huazhong University of Science and Technology
2016-2025
Tongji Hospital
2015-2025
Wuhan University
2008-2025
Renmin Hospital of Wuhan University
2025
China Geological Survey
2023-2025
Ministry of Natural Resources
2024-2025
Hunan Normal University
2025
Changsha Normal University
2025
Nanjing Drum Tower Hospital
2013-2024
Union Hospital
2024
Despite past extensive studies, the mechanisms underlying pulmonary fibrosis (PF) still remain poorly understood. Here, we demonstrated that lungs originating from different types of patients with PF, including coronavirus disease 2019, systemic sclerosis-associated interstitial lung disease, and idiopathic mice following bleomycin (BLM)-induced PF are characterized by altered methyl-CpG-binding domain 2 (MBD2) expression in macrophages. Depletion Mbd2 macrophages protected against...
Abstract Aberrant amino acid metabolism is a common event in obesity. Particularly, subjects with obesity are characterized by the excessive plasma kynurenine (Kyn). However, primary source of Kyn and its impact on metabolic syndrome yet to be fully addressed. Herein, we show that overexpressed indoleamine 2,3-dioxygenase 1 (IDO1) adipocytes predominantly contributes Kyn, indicating central role metabolism. Depletion Ido1 abrogates accumulation, protecting mice against Mechanistically,...
Tissue injury triggers complex communication between cells via secreted signaling molecules such as cytokines and growth factors. Discerning when where these signals begin how they propagate over time is very challenging with existing cell culture analysis tools. The goal of this study was to develop new tools in the form microfluidic co-cultures integrated biosensors for local continuous monitoring signals. Specifically, we focused on alcohol affects TGF-β two liver types, hepatocytes...
Doxorubicin (DOX) is one of the most potent anticancer agents in cancer chemotherapy, but clinical use DOX restricted by its severe side effects caused nonspecific delivery. To alleviate and improve antitumor efficacy DOX, a novel redox-sensitive hydroxyethyl starch-doxorubicin conjugate, HES-SS-DOX, with diameter 19.9 ± 0.4 nm was successfully prepared for tumor targeted drug delivery GSH-mediated intracellular release. HES-SS-DOX relatively stable under extracellular GSH level (∼2 μM)...
Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection analysis tumor necrosis factor-alpha (TNF-α) – a key inflammatory cytokine in whole human blood. When testing spiked blood, TNF-α limit 58 pM (10 ng mL−1) linear range 6 nM (100 could be achieved. Furthermore, mitogen stimulated blood was demonstrated.
Paclitaxel (PTX) is an effective antineoplastic agent and shows potent antitumor activity against a wide spectrum of cancers. Yet, the clinical use PTX limited by its poor aqueous solubility side effects associated with current therapeutic formulation. To tackle these obstacles, we report, for first time, α-amylase- redox-responsive nanoparticles based on hydroxyethyl starch (HES) tumor-targeted delivery PTX. conjugated onto HES redox-sensitive disulfide bond to form HES–SS-PTX, which was...
Abstract Background Tumor recurrence and metastasis occur at a high rate in patients with colon cancer. Identification of effective strategies for the treatment cancer is critical. Recently, poly (lactic-co-glycolic acid) (PLGA) has been shown to have potential as broad therapeutic drug delivery system. We designed dual-loaded nanoparticle system overcome limitations chemotherapeutic drugs used treat Methods developed epidermal growth factor (EGF) functionalized PLGA nanoparticles (NPs)...
Idiopathic pulmonary fibrosis (IPF) is a chronic and diffuse form of interstitial lung disease unknown etiology with fatal outcome. Although various strategies for IPF have been developed over the last few decades, no significant positive impact on prognosis has observed. According to current paradigm, macrophages recognized play role in pathogenesis. Here, we report potential nanomedicine-based gene therapy based regulate macrophage polarization. Method: C57BL/6 mice were obtained used...
Abstract Objectives Hypoxia is an important risk factor for pulmonary arterial remodelling in hypertension (PAH), and the Janus kinase 2 (JAK2) believed to be involved this process. In present report, we aimed investigate role of JAK2 vascular smooth muscle cells during course PAH. Methods Smooth cell (SMC)‐specific Jak2 deficient mice their littermate controls were subjected normobaric normoxic or hypoxic (10% O ) challenges 28 days monitor development PAH, respectively. To further...
Sepsis refers to the systemic inflammatory response syndrome caused by infection. It is a major clinical problem and cause of death for patients in intensive care units worldwide. The Fat mass obesity-related protein (FTO) primary N 6 -methyladenosine demethylase. However, role FTO pathogenesis diseases remains unclear. We herein show that nanoparticle-mediated Fto -siRNA delivery or inhibitor entacapone administration dramatically inhibited macrophage activation, reduced tissue damage...
Although DNA methylation has been recognised in the pathogenesis of idiopathic pulmonary fibrosis (IPF), exact mechanisms are yet to be fully addressed. Herein, we demonstrate that lungs originated from IPF patients and mice after bleomycin (BLM)-induced characterised by altered along with overexpression myofibroblasts methyl-CpG-binding domain 2 (MBD2), a reader responsible for interpreting methylome-encoded information. Specifically, depletion Mbd2 fibroblasts or protected BLM-induced...
Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease with limited therapeutic options. Tartrate-resistant acid phosphatase 5 (ACP5) performs variety of functions. However, its role in IPF remains unclear. Here, we demonstrate that the levels ACP5 are increased patient samples and mice bleomycin (BLM)-induced fibrosis. In particular, higher present sera patients diffusing capacity lungs for carbonmonoxide (DLCO) less than 40% predicted value. Additionally, Acp5 deficiency...
Among different age groups, middle-aged individuals are particularly susceptible to obesity, with a 22% higher risk of all-cause mortality. However, the underlying mechanisms remain unclear. In this study, we identify adipose progenitor cells (APCs) in white tissue (WAT) subjects as potential causes midlife obesity. Specifically, extracellular vesicles (EVs) derived from APCs display an impaired ability mitigate inflammaging macrophages (ATMs) individuals. Mechanistically, these EVs, lacking...
Selective drug release is highly desirable for photothermal/chemo combination therapy when two or even more theranostic agents are encapsulated together within the same nanocarrier. A conventional nanocarrier can hardly achieve this goal. Herein, doxorubicin and indocyanine green (DOX/ICG)-loaded nanocolloidosomes (NCs), with selective release, were fabricated as a novel multifunctional nanoplatform therapy. Templating from galactose-functionalized hydroxyethyl starch-polycaprolactone...
We developed a cell-culture/biosensor platform consisting of aptamer-modified Au electrodes integrated with reconfigurable microfluidics for monitoring transforming growth factor-beta 1 (TGF-β1), an important inflammatory and pro-fibrotic cytokine. Aptamers were thiolated, labeled redox reporters, self-assembled on gold surfaces. The biosensor was determined to be specific TGF-β1 experimental detection limit ng/mL linear range extending 250 ng/mL. Upon determining figures merit, aptasensor...
Abstract Type 1 diabetes (T1D) is characterized by the selective autoimmune destruction of islet β cells, and macrophages play a significant role in this process. Small ubiquitin-like modification (SUMOylation) an important posttranslational involved T1D pathogenesis, but its function remains unexplored. We presently developed used macrophage-specific ubiquitin-conjugating enzyme E2 ( Ubc9 ) knockout (LyzM-Cre -Ubc9 fl/fl , KO) mice to address impact SUMOylation on macrophage model. observed...
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fatal interstitial lung disease characterized by abnormal transition proliferation of fibroblasts. The uncontrolled fibroblasts, commonly known as myofibroblasts, are the principal source enormous extracellular matrix (ECM) depositing in parenchyma, leading to gradual failure gas exchange mortality patients. However, up now, rare effective therapeutic strategies have been developed blockade fibroblast-to-myofibroblast (FMT)...
Background Thrombocytopenia has been proved to be associated with hospital mortality in patients severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. However, the detailed association of thrombocytopenia subsequent progression organ functions and long-term prognosis critically ill COVID-19 remains explored. Methods Medical records 167 confirmed cases from February 16 March 21, 2020 were collected this two-center retrospective study. 180-day’s outcome clinical development...
Abstract Kdm2a catalyzes H3K36me2 demethylation to play an intriguing epigenetic regulatory role in cell proliferation, differentiation, and apoptosis. Herein we found that myeloid-specific knockout of ( LysM- Cre- f/f , −/− ) promoted macrophage M2 program by reprograming metabolic homeostasis through enhancing fatty acid uptake lipolysis. increased levels at the Pparg locus along with augmented chromatin accessibility Stat6 recruitment, which rendered macrophages preferential polarization....
Doxorubicin (DOX), a kind of wide-spectrum chemotherapeutic drug, can cause severe side effects in clinical use. To enhance its antitumor efficacy while reducing the effects, two kinds nanoparticles with desirable compositions and properties were assembled using optimally synthesized hydroxyethyl starch-grafted-polylactide (HES-g-PLA) copolymers utilized as partner nanocarriers. The large empty HES-g-PLA (mean size, ca. 700 nm), at an optimized dose 400 mg/kg, used to block up...