A5010684870- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Advanced Fluorescence Microscopy Techniques
- Nuclear Structure and Function
- Enzyme Structure and Function
- Spaceflight effects on biology
- Biochemical and Molecular Research
- Nitric Oxide and Endothelin Effects
- Plant Molecular Biology Research
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Amino Acid Enzymes and Metabolism
- bioluminescence and chemiluminescence research
- Advanced NMR Techniques and Applications
- Organometallic Compounds Synthesis and Characterization
- Insect and Arachnid Ecology and Behavior
- Cellular transport and secretion
- Physiological and biochemical adaptations
- Telomeres, Telomerase, and Senescence
- Laser-Ablation Synthesis of Nanoparticles
- Genetics, Aging, and Longevity in Model Organisms
- Protist diversity and phylogeny
- Optical Coherence Tomography Applications
Harvard University
2014-2024
Center for Systems Biology
2016-2018
Nanjing Medical University
2010-2016
National Taiwan University
2005-2009
Deakin University
1993
Understanding the coordination of cell-division timing is one outstanding questions in field developmental biology. One active control parameter cell-cycle duration temperature, as it can accelerate or decelerate rate biochemical reactions. However, controlled experiments at cellular scale are challenging, due to limited availability biocompatible temperature sensors, well lack practical methods systematically local temperatures and dynamics. Here, we demonstrate a method probe...
Telomere and telomerase alterations have been reported in mood disorders. However, the role of depression remains unclear. Here we show that chronic mild stress (CMS) led to a significant decrease reverse transcriptase (TERT) level activity hippocampus. Treatment with antidepressant fluoxetine reversed CMS-induced TERT changes. Inhibiting by systemic administration (100 mg · kg −1 d , i.p., for 14 d), intrahippocampal microinjection (0.7 μmol, 2 μl), or infusion (using an osmotic minipump,...
Spindle microtubules, whose dynamics vary over time and at different locations, cooperatively drive chromosome segregation. Measurements of microtubule spindle ultrastructure can provide insight into the behaviors helping elucidate mechanism Much work has focused on organization kinetochore that is, region between chromosomes poles. In comparison, microtubules in central-spindle region, segregating chromosomes, have been less thoroughly characterized. Here, we report measurements movement...
Recent work done exclusively in tissue culture cells revealed that the nuclear envelope (NE) ruptures and repairs interphase. The duration of NE depends on lamins; however, underlying mechanisms relevance to vivo events are not known. Here, we use Caenorhabditis elegans zygote analyze lamin’s role rupture repair vivo. Transient subsequent collapse induced by weaknesses lamina caused expression an engineered hypomorphic C. lamin allele. Dynein-generated forces position nuclei enhance severity...
It has been demonstrated that neuronal nitric oxide synthase (nNOS) negatively regulates adult neurogenesis. However, the cellular and molecular mechanisms underlying are poorly understood. Here, we show nNOS from neural stem cells (NSCs) neurons play opposite role in regulating The NSCs treated with inhibitor N(5)-(1-imino-3-butenyl)-L- ornithine (L-VNIO) or gene deletion exhibited significantly decreased proliferation differentiation, indicating NSCs-derived is essential for cocultured...
During eukaryotic cell division, chromosomes are linked to microtubules (MTs) in the spindle by a macromolecular complex called kinetochore. The bound kinetochore (KMTs) crucial ensuring accurate chromosome segregation. Recent reconstructions electron tomography (Kiewisz et al., 2022) captured positions and configurations of every MT human mitotic spindles, revealing that roughly half KMTs these spindles do not reach pole. Here, we investigate processes give rise this distribution using...
The forces which orient the spindle in human cells remain poorly understood due to a lack of direct mechanical measurements mammalian systems. We use magnetic tweezers measure force on mitotic spindles. Combining spindle's measured resistance rotation, speed it rotates after laser ablating astral microtubules, and estimates number ablated microtubules reveals that each microtubule contacting cell cortex is subject ~1 pN pulling force, suggesting pulled by an individual dynein motor. find...
ABSTRACT Few techniques are available for elucidating the nature of forces that drive subcellular behaviors. Here we develop two complementary ones: 1) femtosecond stereotactic laser ablation (FESLA), which rapidly creates complex cuts structures, thereby allowing precise dissection when, where, and in what direction generated; 2) assessment fluid flows, by comparing direct flow measurements, using microinjected fluorescent nanodiamonds, to large-scale fluid-structure simulations different...
Abstract Spindle microtubules, whose dynamics vary over time and at different locations, cooperatively drive chromosome segregation. Measurements of microtubule spindle ultrastructure can provide insight into the behaviors helping elucidate mechanism Much work has focused on organization kinetochore i.e. region between chromosomes poles. In comparison, microtubules in central region, segregating chromosomes, have been less thoroughly characterized. Here, we report measurements movement...
ABSTRACT During eukaryotic cell division, chromosomes are linked to microtubules (MTs) in the spindle by a macromolecular complex called kinetochore. The bound kinetochore (KMTs) crucial ensuring accurate chromosome segregation. Recent electron tomography reconstructions (Kiewisz et al. 2021) captured positions and configurations of every MT human mitotic spindles, revealing that many KMTs these spindles do not reach pole. Here, we investigate processes give rise this distribution using...
Abstract Recent work done exclusively in tissue culture cells revealed that the nuclear envelope (NE) undergoes ruptures leading to transient mixing of and cytoplasmic components. The duration NE depends on lamins, however underlying mechanisms relevance vivo events is not known. Here, we use C. elegans embryos show dynein forces position nuclei increase severity lamin-induced . In absence forces, lamin prevents nuclear-cytoplasmic caused by ruptures. By monitoring dynamics rupture events,...
Two-photon autofluorescence and second harmonic generation (SHG) microscopy are useful imaging techniques for studying tissue components. In this work, we apply modality to study porcine eye. particular, use SHG investigate the structural changes excised corneas found that technique is its under thermal treatment.
This purpose of this study is to demonstrate the feasibility using multiphoton microscopy in imaging eye surface. Specifically, cornea, limbus, conjunctiva, and sclera were imaged induced fluorescence second-harmonic generation (SHG) imaging.