A5063457623- Dermatology and Skin Diseases
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Wound Healing and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Contact Dermatitis and Allergies
- Urticaria and Related Conditions
- Hidradenitis Suppurativa and Treatments
- Skin Protection and Aging
- Microscopic Colitis
- Pressure Ulcer Prevention and Management
- Immune Response and Inflammation
- Circadian rhythm and melatonin
- Psoriasis: Treatment and Pathogenesis
- Colorectal and Anal Carcinomas
- Chemokine receptors and signaling
- Inflammasome and immune disorders
- Oral Health Pathology and Treatment
- Herpesvirus Infections and Treatments
- Acne and Rosacea Treatments and Effects
- Antimicrobial Peptides and Activities
- Hair Growth and Disorders
- Asthma and respiratory diseases
Janssen (Belgium)
2024
Duke University
2016-2023
Janssen (United States)
2022-2023
Duke Medical Center
2015-2021
Duke University Hospital
2015-2021
UK Astronomy Technology Centre
2018
Scripps Research Institute
2011-2017
Hidradenitis suppurativa (HS) is a debilitating chronic inflammatory skin disease characterized by abscess formation and development of multiple draining sinus tracts in the groin, axillae, perineum. Using proteomic transcriptomic approaches, we responses HS depth, revealing immune centered on IFN-γ, IL-36, TNF, with lesser contribution from IL-17A. We further identified B cells plasma cells, associated increases immunoglobulin production complement activation, as pivotal players...
The epidermis, the outer layer of skin, forms a physical and antimicrobial shield to protect body from environmental threats. Skin injury severely compromises epidermal barrier requires immediate repair. Dendritic T cells (DETC) reside in murine epidermis where they sense skin serve as regulators orchestrators immune responses. Here, we determined that TCR stimulation induces IL-17A production by subset DETC. This IL-17A-producing DETC was distinct IFN-γ producers, despite similar surface...
TRPV4 ion channels function in epidermal keratinocytes and innervating sensory neurons; however, the contribution of channel either cell to neurosensory remains be elucidated. We recently reported as a critical component keratinocyte machinery that responds ultraviolet B (UVB) functions critically convert into pain-generator after excess UVB exposure. One key mechanism was increased expression secretion endothelin-1, which is also known pruritogen. Here we address question whether skin itch,...
Easy M Our immune systems can produce a vastly diverse repertoire of antibody molecules that each recognize and bind to specific foreign antigen via hypervariable region. However, there are few bacterial antigens—such as Protein A, G, L—that instead the antibody's conserved regions large number different antibodies. These high-affinity broad-spectrum antibody-binding properties have been widely exploited both in laboratory industry for purifying, immobilizing, detecting Grover et al. (p. 656...
Abstract Substances that penetrate the skin surface can act as allergens and induce a T cell–mediated inflammatory disease called contact hypersensitivity (CHS). IL-17 is key cytokine in CHS was originally thought to be produced solely by CD4+ cells. However, it now known several cell types, including γδ cells, produce IL-17. In this study, we determine role of especially dendritic epidermal cells (DETCs), CHS. Using well-established model for which 2,4-dinitrofluorobenzene (DNFB) used...
Abstract Purpose: Cutaneous squamous cell carcinoma (cSCC) is the most common human cancer with metastatic potential. Despite T cells accumulating around cSCCs, these tumors continue to grow and persist. To investigate reasons for failure of mount a protective response in cSCC, we focused on regulatory (Tregs) as this suppressive population well represented among infiltrating lymphocytes. Experimental Design: Flow cytometry was conducted cSCC lymphocytes vitro functional assays were...
The cutaneous wound-healing program is a product of complex interplay among diverse cell types within the skin. One fundamental process that mediated by these reciprocal interactions mobilization local stem pools to promote tissue regeneration and repair. Using ablation epidermal caspase-8 as model wound healing in Mus musculus, we analyzed signaling components responsible for epithelial proliferation. We found IL-1α IL-7 secreted from keratinocytes work tandem expand activated population...
Anaphylactic reactions are triggered when allergens enter the blood circulation and activate immunoglobulin E (IgE)-sensitized mast cells (MCs), causing systemic discharge of prestored proinflammatory mediators. As MCs extravascular, how they perceive circulating remains a conundrum. Here, we describe existence CD301b+ perivascular dendritic cell (DC) subset that continuously samples relays antigens to neighboring MCs, which vigorously degranulate trigger anaphylaxis. DC antigen transfer...
Skin-resident T cells have been shown to play important roles in tissue homeostasis and wound repair, but their role UV radiation (UVR)-mediated skin injury subsequent regeneration is less clear. In this study, we demonstrate that acute UVR rapidly activates skin-resident humans dendritic epidermal γδ (DETCs) mice through mechanisms involving the release of ATP from keratinocytes. Following UVR, extracellular leads an increase CD69 expression, proliferation, IL-17 production, changes DETC...
Hidradenitis suppurativa (HS) is a debilitating chronic inflammatory skin disease resulting in non-healing wounds affecting body areas of high hair follicle and sweat gland density. The pathogenesis HS not well understood but appears to involve dysbiosis-driven aberrant activation the innate immune system leading excessive inflammation. Marked dysregulation antimicrobial peptides proteins (AMPs) observed, which may contribute this sustained Here, we analyzed transcriptomes from previously...
Activin is an important orchestrator of wound repair, but its potential role in skin carcinogenesis has not been addressed. Here we show using different types genetically modified mice that enhanced levels activin the promote tumour formation and their malignant progression through induction a pro-tumourigenic microenvironment. This includes accumulation tumour-promoting Langerhans cells regulatory T epidermis. Furthermore, inhibits proliferation tumour-suppressive epidermal γδ cells,...
Skin wound repair requires a coordinated program of epithelial cell proliferation and differentiation as well resistance to invading microbes. However, the factors that trigger in this inflammatory process are incompletely understood. In study, we demonstrate IL-27 is rapidly transiently produced by CD301b+ cells skin after injury. The functional role demonstrated finding CD301b-depleted mice exhibit delayed closure vivo, which could be rescued topical treatment. Furthermore, genetic...
Neutrophils obstruct the meibomian gland in a mouse model of inflammatory eye disease and correlate with severity patients dysfunction.
Small-molecule biased agonists of a chemokine receptor elicit distinct physiological outcomes.
IL-27 signaling activates the production of antiviral proteins in skin and inhibits Zika virus infection.
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by recurrent abscesses, nodules, and sinus tracts in areas of high hair follicle sweat gland density. These can present with purulent drainage scar formation. Dysregulation multiple immune pathways drives the complexity HS pathogenesis may account for heterogeneity treatment response patients. Using transcriptomic approaches, including single-cell sequencing protein analysis, we here characterize innate...
Abstract The innate immune components that modulate allergic contact hypersensitivity (CHS) responses are poorly defined. Using human skin from dermatitis patients and a mouse model of CHS, we find hapten allergens disrupt the Arginase1 (Arg1) inducible NO synthase (iNOS) dynamic in monocytes/macrophages (mono/MΦ), which renders those cells ineffectual suppressing inflammation. Mice lacking Arg1 MΦ develop increased CHS characterized by elevated ear thickening, mono/MΦ−dominated dermal...