A5050746647- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Cardiac and Coronary Surgery Techniques
- Infectious Aortic and Vascular Conditions
- Aortic Disease and Treatment Approaches
- Ion channel regulation and function
- Diabetes Management and Research
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Infective Endocarditis Diagnosis and Management
- Renal and Vascular Pathologies
- Vascular Procedures and Complications
- Central Venous Catheters and Hemodialysis
- stochastic dynamics and bifurcation
- Reconstructive Surgery and Microvascular Techniques
- Diabetes and associated disorders
- Congenital heart defects research
- Congenital Heart Disease Studies
Humacyte (United States)
2017-2023
Duke University
2009-2018
Triangle
2017
Traditional vascular grafts constructed from synthetic polymers or cadaveric human animal tissues support the clinical need for readily available blood vessels, but often come with associated risks. Histopathological evaluation of these materials has shown adverse host cellular reactions and/or mechanical degradation due to insufficient inappropriate matrix remodeling. We developed an investigational bioengineered acellular vessel (HAV), which is currently being studied as a hemodialysis...
Abstract Transdifferentiation has been described as a novel method for converting human fibroblasts into induced cardiomyocyte-like cells. Such an approach can produce differentiated cells to study physiology or pathophysiology, examine drug interactions toxicities, and engineer cardiac tissues. Here we describe the transdifferentiation of dermal towards cell lineage via expression transcription factors GATA4, TBX5, MEF2C, MYOCD, NKX2–5, delivery microRNAs miR-1 miR-133a. Cells undergoing...
Synthetic expanded polytetrafluorethylene (ePTFE) grafts are routinely used for vascular repair and reconstruction but prone to sustained bacterial infections. Investigational bioengineered human acellular vessels (HAVs) have shown clinical success may confer lower susceptibility infection. Here we directly compared the of ePTFE HAV contamination in a preclinical model infection.Sections (1 cm2) (n = 42) or were inserted within bilateral subcutaneous pockets on dorsum rats inoculated with...
Abstract The ability to directly enhance electrical excitability of human cells is hampered by the lack methods efficiently overexpress large mammalian voltage-gated sodium channels (VGSC). Here we describe use small prokaryotic (BacNa v ) create de novo excitable tissues and augment impaired action potential conduction in vitro . Lentiviral co-expression specific BacNa orthologues, an inward-rectifying potassium channel, connexin-43 primary fibroblasts from heart, skin or brain yields...
Synthetic expanded polytetrafluorethylene (ePTFE) grafts are known to be susceptible bacterial infection. Results from preclinical and clinical studies of bioengineered Human Acellular Vessels (HAV) have shown relatively low rates This study evaluates the interactions human neutrophils bacteria with ePTFE HAV vascular conduits determine whether there is a correlation between neutrophil-conduit observed differences their infectivity in vivo. A Phase 3 comparative investigational HAVs (n=177)...
To understand how excitable tissues give rise to arrhythmias, it is crucially necessary the electrical dynamics of cells in context their environment. Multicellular monolayer cultures have proven useful for investigating arrhythmias and other conduction anomalies, because relatively simple structure, these constructs lend themselves paired computational studies that often help elucidate mechanisms observed behavior. However, tissue cardiomyocyte monolayers currently require use neonatal with...
Islet transplantation into the portal vein is currently regarded as most promising therapeutic approach for reversing Type-1 diabetes. Nevertheless, islet loss due to inflammatory reaction, hypoxia, and peri-islet thrombosis, often hinders sustained insulin independence in patients, despite considerable number of islets utilized. In response these constraints, we are focused on development an acellular biological vascular graft with outer layer coated islet-populated hydrogel known...
Electrophysiological mismatch between host cardiomyocytes and donor cells can directly affect the electrical safety of cardiac cell therapies; however, ability to study host-donor interactions at microscopic scale in situ is severely limited. We systematically explored how action potential (AP) differences other excitable modulate vulnerability conduction failure vitro.AP propagation was optically mapped 75 μm resolution micropatterned strands (n=152) which neonatal rat ventricular myocytes...
BACKGROUND This study evaluated performance of a tissue-engineered human acellular vessel (HAV) in porcine model acute vascular injury and ischemia. The HAV is an engineered blood consisted extracellular matrix proteins. Limb reperfusion outcomes the were compared with those from synthetic expanded polytetrafluoroethylene (ePTFE) grafts. METHODS Thirty-six pigs randomly assigned to four treatment groups, receiving either or PTFE graft following hind limb ischemia period 0 6 hours. All grafts...
The use of genetic engineering to induce or alter specific protein expression in stem cells has already facilitated research this field and may, additionally, offer a potential route for designing more efficient cell sources cardiac repair. As the feasibility manipulation been proved as techniques continue advance, combinatorial approach therapy is promising. This review will thus describe applications improve isolation, selection, differentiation prior implantation well strategies promote...
Palliative treatment of cyanotic congenital heart disease (CCHD) uses systemic-to-pulmonary conduits, often a modified Blalock-Taussig-Thomas shunt (mBTTs). Expanded polytetrafluoroethylene (ePTFE) mBTTs have associated risks for thrombosis and infection. The Human Acellular Vessel (HAV) (Humacyte, Inc) is decellularized tissue-engineered blood vessel currently in clinical trials adults vascular trauma, peripheral artery disease, end-stage renal requiring hemodialysis. In addition to...
Objective: Conduit for coronary artery bypass grafting (CABG) can be limited due to poor quality or prior removal of saphenous veins. A human acellular vessel (HAV) bioengineered from vascular cells and then decellularized implantation may provide a readily available, off-the-shelf conduit CABG. Here we evaluate the use small diameter HAV CABG in non-human primate model. Methods: Adult male baboons (n=7; 26.8 - 37.0 kg) were imaged by left heart catheterization computed tomography...
Introduction: Limitations of coronary artery bypass grafting (CABG) include the availability, quality, and long-term patency autologous saphenous vein grafts. An “off-the-shelf” human acellular vessel (HAV) bioengineered from banked vascular cells, decellularized before implantation, capable host cellular regeneration may address these issues. Therefore, we assessed early tolerability a small diameter HAV as CABG conduit in baboon surgical model. Methods: Adult male, non-immunosuppressed...
Cyanotic congenital heart disease (CCHD) due to one or more structural abnormalities causes insufficient blood oxygenation. Approximately 2 out of every 1000 infants are born with CCHD and palliative treatment for many patients involves shunting from the systemic pulmonary circuits, often through a modified Blalock-Taussig-Thomas shunt (BTTs). Synthetic vascular grafts typically used BTTs have associated risks intimal hyperplasia, thrombosis, infection. Humacyte, Inc. has developed 6mm...