A5100711506- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Spectroscopy Techniques in Biomedical and Chemical Research
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Adenosine and Purinergic Signaling
- RNA Research and Splicing
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Traumatic Brain Injury and Neurovascular Disturbances
- Immune cells in cancer
- Macrophage Migration Inhibitory Factor
- Monoclonal and Polyclonal Antibodies Research
- Wnt/β-catenin signaling in development and cancer
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- RNA modifications and cancer
- Kruppel-like factors research
- Congenital heart defects research
- FOXO transcription factor regulation
- Identification and Quantification in Food
- HER2/EGFR in Cancer Research
- Congenital Heart Disease Studies
University of Michigan
2014-2025
Michigan Medicine
2020-2025
Huazhong Agricultural University
2023
Hunan Normal University
2004-2022
Shandong University
2022
Shandong Provincial QianFoShan Hospital
2022
Xinjiang Academy of Animal Science
2021
Arbor Research Collaborative for Health
2020
Michigan United
2019
United States Department of Defense
2018
The MTOC is “speck”-tacular Inflammasome complexes are formed in response to pathogen-associated molecules. They initiate both the maturation of inflammatory cytokines and pyroptosis, a type programmed cell death. One notable feature for inflammasome activation formation single supramolecular punctum (or “speck”) each affected cell. However, location mechanism speck poorly understood. Magupalli et al. report that NLRP3- pyrin-mediated inflammasomes, their assembly downstream functions occur...
The DF3/MUC1 mucin-like glycoprotein is aberrantly overexpressed in human breast carcinomas. functional role of DF3 unknown. present studies demonstrate that associates with β-catenin. Similar findings have been obtained for γ-catenin but not α-catenin. DF3, like E-cadherin and the adenomatous polyposis coli gene product, contains an SXXXXXSSL site responsible direct binding to results further interaction β-catenin dependent on cell adhesion. These signaling support a adhesion epithelial...
The DF3/MUC1 mucin-like, transmembrane glycoprotein is aberrantly overexpressed in most human carcinomas. MUC1 cytoplasmic domain interacts with the c-Src tyrosine kinase and thereby increases binding of β-catenin. In present work, coimmunoprecipitation studies demonstrate that associates constitutively epidermal growth factor receptor (EGF-R) ZR-75-1 breast carcinoma cells. Immunofluorescence show EGF-R associate at cell membrane. We also activated phosphorylates tail on a YEKV motif...
The DF3/MUC1 mucin-like glycoprotein is highly overexpressed in human carcinomas. Recent studies have demonstrated that the cytoplasmic domain of MUC1 interacts with β-catenin. Here we show associates glycogen synthase kinase 3β (GSK3β). GSK3β binds directly to an STDRSPYE site and phosphorylates serine adjacent proline. Phosphorylation by decreases binding β-catenin vitro vivo. GSK3β-mediated phosphorylation had no apparent effect on levels or transcriptional coactivation function results,...
The DF3/MUC1 mucin-like glycoprotein is aberrantly overexpressed in most human carcinomas. cytoplasmic domain of MUC1 interacts with glycogen synthase kinase 3β (GSK3β) and thereby decreases binding β-catenin. present studies demonstrate that associates the c-Src tyrosine kinase. phosphorylates at a YEKV motif located between sites involved interactions GSK3β results SH2 binds directly to pYEKV inhibits interaction GSK3β. Moreover contrast GSK3β, vitro andin vivo c-Src-mediated...
BACKGROUND We recently demonstrated that suberoylanilide hydroxamic acid, a broad-spectrum histone deacetylase (HDAC) inhibitor inhibits HDACs 1, 2, 3, and 6, improves survival in mouse model of cecal ligation puncture (CLP)–induced lethal sepsis. The current study was undertaken to determine the effect selective inhibition HDAC isoform on survival, key cytokine production, organ injury, bacteria clearance, cell apoptosis. METHODS In Experiment C57BL/6J mice were subjected CLP and, 1 hour...
MicroRNAs are small non-coding RNAs that may also function as oncogenes and tumor suppressor genes, the abnormal expression of microRNAs is associated with various human tumors. MicroRNA-145 (miR-145) inhibits growth increases chemo- or radiosensitivity in cancers. However, role miR-145 breast cancer progression remains unknown. In this study, level was measured via quantitative real-time PCR 88 pairs tissues adjacent normal a panel cell lines. Cell proliferation migration were assessed by...
Peptidylarginine deiminases (PADs) are a family of calcium-dependent enzymes that involved in variety human disorders, including cancer and autoimmune diseases. Although targeting PAD4 has shown no benefit sepsis, the role PAD2 remains unknown. Here, we report is engaged sepsis sepsis-induced acute lung injury both patients mice. Pad2–/– or selective inhibition by small molecule inhibitor increased survival improved overall outcomes mouse models sepsis. Pad2 deficiency decreased neutrophil...
Traumatic brain injury and hemorrhagic shock (TBI+HS) elicit a complex inflammatory response that contributes to secondary injury. There is currently no proven pharmacologic treatment for TBI+HS, but modulation of the epigenome has been shown be promising strategy. The aim this study was investigate whether valproic acid (VPA), histone deacetylase inhibitor, modulates expression cerebral gene profiles in large animal model TBI+HS. Ten Yorkshire swine were subjected computer-controlled TBI+HS...
Adulteration of higher priced milks with cheaper ones to obtain extra profit can adversely affect consumer health and the market. In this study, pure buffalo milk (BM), goat (GM), camel (CM), their mixtures 5–50% (vol/vol) cow or water were used. Mid-infrared spectroscopy (MIRS) combined modern statistical machine learning was used for discrimination quantification adulteration in BM, GM, CM. Compared partial least squares (PLS), learning—especially support vector machines (SVM), projection...
Abstract Bacterial infections leading to sepsis are a major cause of deaths in the intensive care unit. Unfortunately, no effective methods available capture early onset infectious near patient with both speed and sensitivity required for timely clinical treatment. To fill gap, authors develop highly miniaturized (2.5 × 2.5 µm 2 ) plasmo‐photoelectronic nanostructure device that detected citrullinated histone H3 (CitH3), biomarker released blood circulatory system by neutrophils. Rapidly...
We have shown that administration of mesenchymal stem cell-derived exosomes (single dose given within 1 hour) in models traumatic brain injury (TBI) and hemorrhagic shock is neuroprotective. The precise mechanisms responsible for the neuroprotection are not fully understood. This study was designed to investigate transcriptomic changes associated with this treatment strategy.Yorkshire swine (40-45 kg) were subjected a severe TBI (12-mm cortical impact) (40% estimated total blood volume). One...
Abstract Mediating protein citrullination, peptidyl arginine deiminase 2 (PAD2) has recently been reported to influence macrophage phenotypes. However, the mechanisms of PAD2 on function in Pseudomonas aeruginosa (PA)‐induced acute lung injury syndrome (ALI) remains unclear. Utilizing single‐cell RNA sequencing and mass spectrometry‐based proteomics, a new citrullination site at 171 (R171) is discovered within nuclear factor‐ κB (NF‐κB) p65 catalyzed by PAD2, which modulates PAD2‐NF‐κB...
Abstract WD40 repeat proteins play important roles in a variety of cellular functions, including cell growth, proliferation, apoptosis, and intracellular signal transduction. Mitogen‐activated protein kinases (MAPKs) are evolutionary conserved enzymes transduction connecting cell‐surface receptors to critical regulatory targets within cells control survival, adaptation, proliferation. Previous studies revealed that G‐protein coupled (GPCRs) the from extracellular stimuli MAPKs...
Objectives: Hemorrhagic shock (HS) can initiate an exaggerated systemic inflammatory response and multiple organ failure, especially if followed by a subsequent insult ("second hit"). We have recently shown that histone deacetylase inhibitors improve survival in rodent models of HS or septic shock, individually. In the present study, we examined whether valproic acid (VPA), inhibitor, could prolong "two-hit" model: from cecal ligation puncture (CLP). Methods: Male Sprague-Dawley rats...