A5020444758- Organ and Tissue Transplantation Research
- Transplantation: Methods and Outcomes
- Reconstructive Surgery and Microvascular Techniques
- Tissue Engineering and Regenerative Medicine
- Nerve injury and regeneration
- Xenotransplantation and immune response
- Digital Imaging in Medicine
- Organ Transplantation Techniques and Outcomes
- Reproductive System and Pregnancy
- Renal Transplantation Outcomes and Treatments
- Surgical Sutures and Adhesives
- Nerve Injury and Rehabilitation
- Pain Management and Treatment
- Biomedical Ethics and Regulation
- Tracheal and airway disorders
- Hemodynamic Monitoring and Therapy
- Organ Donation and Transplantation
- Angiogenesis and VEGF in Cancer
- Wound Healing and Treatments
- Central Venous Catheters and Hemodialysis
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Sexual function and dysfunction studies
- T-cell and B-cell Immunology
University of Maryland, Baltimore
2014-2024
University of Maryland Medical Center
2023-2024
Johns Hopkins Medicine
2013-2023
Johns Hopkins University
2013-2023
University of Health Sciences
2018
Naval Medical Research Command
2018
Uniformed Services University of the Health Sciences
2018
Creative Commons
2017
Resonance Research (United States)
2017
Kennedy Krieger Institute
2017
Upon antigen recognition and co-stimulation, T lymphocytes upregulate the metabolic machinery necessary to proliferate sustain effector function. This reprogramming in cells regulates cell activation differentiation but is not just a consequence of recognition. Although such promotes function cells, regulatory employs different reprogramming. Therefore, we hypothesized that inhibition glycolysis glutamine metabolism might prevent graft rejection by inhibiting generation promoting generation....
This study investigates the efficacy of local and intravenous mesenchymal stem cell (MSC) administration to augment neuroregeneration in both a sciatic nerve cut-and-repair rat hindlimb transplant model. Bone marrow-derived MSCs were harvested purified from Brown-Norway (BN) rats. Sciatic transections repairs performed three groups Lewis (LEW) rats: negative controls (n = 4), (epineural) injection systemic (intravenous) 4). Syngeneic (LEW-LEW) 4) allogeneic (BN-LEW) transplants assessed for...
The targeted delivery of therapeutic drugs to lymph nodes (LNs) provides an unprecedented opportunity improve the outcomes transplantation and immune-mediated diseases. high endothelial venule is a specialized segment LN vasculature that uniquely expresses peripheral node addressin (PNAd) molecules. PNAd recognized by MECA79 mAb. We previously generated mAb–coated microparticle (MP) carries tacrolimus. Although this MP trafficked LNs, it demonstrated limited efficacy in our transplant model....
Aberrant excitatory neurotransmission associated with overproduction of glutamate has been implicated in the development HIV-associated neurocognitive disorders (HAND). The glutamine antagonist 6-diazo-5-oxo-l-norleucine (DON, 14) attenuates synthesis HIV-infected microglia/macrophages, offering therapeutic potential for HAND. We show that 14 prevents manifestation spatial memory deficits chimeric EcoHIV-infected mice, a model is not clinically available, however, because its was hampered by...
The pudendal nerve is located topographically in areas which plastic surgeon reconstruct the penis, vagina, perineum, and rectum. This at risk for either compression or direct injury with neuroma formation from obstetrical, urogynecologic, rectal surgery as well pelvic fracture blunt trauma. purpose of this study was to create a 3-dimensional representation based on magnetic resonance imaging pelvis supplemented new anatomic dissections men women delineate location its branches, providing...
Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited application humans, mice has been widely employed for research. The procedure easy learn perform, it does not require delicate microsurgical techniques nor extensive training. Moreover, rejection this occurs very reproducible immunological reaction easily monitored by direct inspection palpation. In addition, secondary with donor-matched or...
Combination therapies that target multiple pathways involved in immune rejection of transplants hold promise for patients need restorative surgery. Herein, a noninteracting multiphase molecular assembly approach is developed to crystallize tofacitinib, potent JAK1/3 inhibitor, within shear-thinning self-assembled fibrillar peptide hydrogel network. The resulting microcrystalline tofacitinib (MTH) can be syringe-injected directly the grafting site during surgery locally deliver small...
In vivo animal model systems, and in particular mouse models, have evolved into powerful versatile scientific tools indispensable to basic translational research the field of transplantation medicine. A vast array reagents is available exclusively this setting, including mono- polyclonal antibodies for both diagnostic interventional applications. addition, a number genotyped, inbred, transgenic, knock out strains allow detailed investigation individual contributions humoral cellular...
Vascularized composite allotransplantation (VCA) is a restorative option for patients suffering from severe tissue defects not amenable to conventional reconstruction. However, the toxicities associated with life-long multidrug immunosuppression enable allograft survival and induce immune tolerance largely limit broader application of VCA. Here, we investigate potential targeted immunomodulation using CTLA4-Ig combined biological porcine-derived extracellular matrix (ECM) scaffold that...
Purpose To demonstrate the feasibility of a miniature handheld optical coherence tomography (OCT) imager for real time intraoperative vascular patency evaluation in setting super-microsurgical vessel anastomosis. Methods A novel Fourier domain Doppler based on 1.3-µm central wavelength swept source extravascular imaging was developed. The minimized through adoption 2.4-mm diameter microelectromechanical systems (MEMS) scanning mirror, additionally 12.7-mm lens system designed and combined...
The immunological barrier currently precludes the clinical utilization of allogeneic stem cells. Although glial-restricted progenitors have become attractive candidates to treat a wide variety neurological diseases, their survival in immunocompetent recipients is limited. In this study, we adopted short-term, systemically applicable co-stimulation blockade-based strategy using CTLA4-Ig and anti-CD154 antibodies modulate T-cell activation context progenitor transplantation. We found that...
Candidates for vascularized composite allotransplantation (VCA) are frequently sensitized, putting them at risk antibody-mediated rejection. Current desensitization strategies imperfect and require a living-donor setting. Here we investigated the impact of sensitization on efficacy protocol utilizing syngeneic hematopoietic stem cell transplantation (HSCT) to prevent rejection in VCA.Skin transplants from Dark Agouti Lewis rats were performed sensitization. Orthotopic hind limb donors...
Vascularized composite allotransplantation (VCA) has become a valid therapeutic option to restore form and function after devastating tissue loss. However, the need for high-dose multidrug immunosuppression maintain allograft survival is still hampering more widespread application of VCA. In this study, we investigated immunoregulatory potential costimulation blockade (CoB; CTLA4-Ig anti-CD154 mAb) combined with nonmyeoablative total body irradiation (TBI) promote VCA in fully MHC-mismatched...
Improved nerve regeneration and functional outcomes would greatly enhance the utility of vascularized composite allotransplantation (VCA) such as hand upper extremity transplantation. However, research aimed at achieving this goal has been limited by lack a VCA animal model. We have developed novel rat midhumeral forelimb transplant model that allows for characterization recovery following At final end point 12 weeks, we found animals with transplantation including median, ulnar radial...
We report a nerve graft procedure bridging the thenar branch of median to ulnar in three patients with transection and defect at mid-forearm. Ulnar function was evaluated electroneurography quantitative sensory-motor testing before after surgery, 6-year follow-up. After surgery all showed electroneurographic evidence innervation intrinsic muscles normally innervated by nerve. The average strength Grade 4 originally supplied final Our results indicate that may support regeneration so help...
Postamputation pain is a spectrum of debilitating sensations that impacts millions people in the United States. While development postamputation pain, including phantom limb (PLP), multifactorial, it has been associated with disorganized axonal sprouting, resulting neuroma and subsequent central nervous system changes. Nerve reconstruction surgeries, such as regenerative peripheral nerve interface (RPNI) targeted muscle reinnervation (TMR), provide transected fibers proper target organs for...
Background: Congenital and acquired chest wall deformities represent a significant challenge to functional reconstruction may impact feasibility of heart transplantation for patients with end-stage organ failure. In the recent past, concept replacing like-with-like tissue by using vascularized composite allografts (VCA) has been enthusiastically employed complex defects. Methods: this study, we introduce novel murine model en bloc wall, heart, thymus thereby use both defects also...
Transplant tolerance in the absence of long-term immunosuppression has been an elusive goal for solid organ transplantation. Recently, it become clear that metabolic reprogramming plays a critical role promoting T cell activation, differentiation and function. Targeting metabolism can preferentially inhibit effector generation while simultaneously regulatory cells. With this mind, we hypothesized costimulatory blockade with CTLA4-Ig combination targeting might promote induction tolerance.
Exploration of novel strategies in organ transplantation to prolong allograft survival and minimizing the need for long-term maintenance immunosuppression must be pursued. Employing vascularized bone marrow co-transplantation thymus have shown promise this regard various animal models.1-11 Vascularized allows uninterrupted transfer donor cells within preserved microenvironment, incorporation tissue with has increase T-cell chimerism ultimately playing a supportive role induction immune...
Penis transplantation represents an exciting new avenue for restoration of male urogenitalia. However, little is known about the specific immunological features penile transplants, limiting their application in complex urogenital reconstruction. To properly study this emerging form transplantation, adequate preclinical models are a necessity. The purpose to establish clinical and histological rejection classification tissue transplants using rat heterotopic transplant model that includes...