A5032739826- Glycosylation and Glycoproteins Research
- Tracheal and airway disorders
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Airway Management and Intubation Techniques
- Infant Nutrition and Health
- Surgical Simulation and Training
- Anatomy and Medical Technology
- Plasmonic and Surface Plasmon Research
- Biosensors and Analytical Detection
- Protein purification and stability
- Lipid Membrane Structure and Behavior
- Reconstructive Facial Surgery Techniques
- Craniofacial Disorders and Treatments
- Digestive system and related health
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Esophageal and GI Pathology
- Nasal Surgery and Airway Studies
- Obstructive Sleep Apnea Research
- Near-Field Optical Microscopy
- Ear Surgery and Otitis Media
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
University of Michigan
2014-2025
University of Wisconsin–Madison
2025
Leibniz Institute of Photonic Technology
2010-2024
Friedrich Schiller University Jena
2021-2024
Helmholtz Institute Jena
2021-2024
C. S. Mott Children's Hospital
2022-2023
Michigan Medicine
2018-2023
Pioneer (United States)
2023
Leibniz Association
2021
The Ohio State University
2015
Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells.The antigen of this antibody in the line, monosialoganglioside, has been isolated.Its carbohydrate structure is probablyThis oligosaccharide sialyl derivative 1acto-Nfucopentaose 11, hapten Le" blood group antigen.About 30 pg ganglioside 1 g cells, wet weight.The was detected autoradiography lipid extracts some...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComplement-dependent damage to liposomes prepared from pure lipids and Forssman haptenStephen C. Kinsky, James A. Haxby, David Zopf, Carl R. Alving, Constance B. KinskyCite this: Biochemistry 1969, 8, 10, 4149–4158Publication Date (Print):October 1, 1969Publication History Published online1 May 2002Published inissue 1 October 1969https://pubs.acs.org/doi/10.1021/bi00838a036https://doi.org/10.1021/bi00838a036research-articleACS PublicationsRequest...
Covalent attachment of polyethylene glycol, PEGylation, has been shown to prolong the half-life and enhance pharmacodynamics therapeutic proteins. Current methods for which rely on chemical conjugation through reactive groups amino acids, often generate isoforms in PEG is attached at sites that interfere with bioactivity. Here, we present a novel strategy site-directed PEGylation using glycosyltransferases attach O-glycans. The process involves enzymatic GalNAc glycosylation specific serine...
To determine the potential of an integrated, image-based computer-aided design (CAD) and 3-dimensional (3D) printing approach to engineer scaffolds for head neck cartilaginous reconstruction auricular nasal reconstruction.
The study demonstrates use of a novel intervention for severe tracheobronchomalacia (TBM).OBJECTIVE To test novel, 3-dimensionally (3D) printed, bioresorbable airway splint efficacy in extending survival porcine model severe, life-threatening TBM.DESIGN AND PARTICIPANTS A randomized, prospective animal trial was used to evaluate an external as treatment TBM multi-institutional, multidisciplinary collaboration between biomedical engineering department and academic surgery center.Six...
A cancer-associated glycolipid antigen
Objectives/Hypothesis To report the clinical safety and efficacy of three‐dimensional (3D)–printed, patient‐specific, bioresorbable airway splints in a cohort critically ill children with severe tracheobronchomalacia. Study Design Case series. Methods From 2012 to 2018, 15 subjects received 29 on their trachea, right and/or left mainstem bronchi. The median age at implantation was 8 months (range, 3–25 months). Nine were female. Five had history extracorporeal membrane oxygenation (ECMO), 11...
ABSTRACT To study carbohydrate-mediated adherence of Streptococcus pneumoniae to the human airway, we measured binding live S. organisms a cultured cell line derived from lining conjunctiva and primary monolayers bronchial epithelial cells in presence absence oligosaccharide inhibitors. Both encapsulated nonencapsulated strains grown mid-logarithmic phase suspension culture adhered respiratory conjunctival line. Adherence nine clinically prevalent capsular types studied was inhibited...
Abstract Plasmonic nanoparticles with spectral properties in the UV‐to‐near‐IR range have a large potential for development of innovative optical devices. Similarly, microstructured fibers (MOFs) represent promising platform technology fully integrated, next‐generation plasmonic devices; therefore, combination MOFs and would open way novel applications, especially sensing applications. In this Full Paper, cost‐effective, nanoparticle layer deposition (NLD) technique is demonstrated...
In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold arrays as sensing platform. spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation target sequence, it will bind on respectively particle spot. This changes local refractive index, which is detected spectroscopically resulting localized surface plasmon resonance (LSPR) peak...
Treatment of blood group A active glycoprotein from human ovarian cyst fluid by one stage Smith degradation followed alkaline ,8-elimination in the presence NaB[3H 4 1 (Carlson degradation) liberated tritiated oligosaccharide alditols.The carbohydrate mixture was fractionated hy gel filtration.elution charcoal.paper chromatography.and high pressure liquid chromatography.Structures were established based on sugar composition, periodate oxidation.methylation analysis, and analysis alditols as...
The mechanical properties of normal auricular cartilage provide a benchmark against which to characterize changes in structure/function due genetic defects creating phenotypic abnormalities collagen subtypes. Such also inputs/targets for reconstruction scaffold design. Several studies report the biomechanical septal, costal, and articular cartilage. However, analogous data are lacking. Therefore, our aim this study was both whole-ear mechanics by mechanically testing specimens fitting...
Significance Algae live in association with microbes that interact by a variety of chemical mediators, resulting mutualistic or antagonistic relationships. Although algae are key contributors to carbon fixation and fundamental for food webs, we still know little about the underlying molecular mechanisms affecting their fitness. This study investigates interaction between an bacterium unicellular alga. It demonstrates multiple roles polyyne, protegencin, is used bacteria attack green algal...
Human milk oligosaccharides are known to play a role in protection against certain infectious diseases. Previous reports indicate that the content of human varies widely among individuals at term but such information on preterm is lacking. After removal fat, protein and most lactose from non-pooled samples, total neutral oligosaccharide fraction was isolated by ion-exchange chromatography followed gel filtration. A Dionex high-performance anion-exchange system equipped with pulsed...
Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered cartilage holds immense potential to surpass current reconstructive options, but limitations clinical translation exist. We endeavored determine viability utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing produce 3D bioscaffolds for tissue engineering. describe a feasibility study revealing novel approach...
Plasmonic nanoparticles, e.g. nanoscale particles consisting of noble metals, show high potential as transducer elements in novel optical sensors. Their properties are based on collective and coherent oscillation the conduction electrons by irradiating electromagnetic waves. The resulting resonance band (localized surface plasmon [LSPR]) is adjustable UV‐ to near‐infrared spectral range can be defined chemical synthesis. synthesis conditions determine dimension, material particle shape,...