A5048145879- Extracellular vesicles in disease
- Diabetes Treatment and Management
- Cardiovascular Disease and Adiposity
- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Adenosine and Purinergic Signaling
- Pancreatic function and diabetes
- Cardiac Ischemia and Reperfusion
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Effects of Exercise
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Biomarkers in Disease Mechanisms
- Biochemical effects in animals
- Oil and Gas Production Techniques
- Long-Term Effects of COVID-19
- Pericarditis and Cardiac Tamponade
- COVID-19 and healthcare impacts
- ATP Synthase and ATPases Research
- Lipid metabolism and disorders
- Chemotherapy-induced cardiotoxicity and mitigation
Rhode Island Hospital
2021-2025
Brown University
2021-2025
Benha University
2024
Providence College
2021-2023
American University of Beirut
2012-2018
Background Sodium-glucose cotransporter-2 inhibitors are cardioprotective independent of glucose control, as demonstrated in animal models acute myocardial ischemia and clinical trials. The functional molecular mechanisms these benefits the setting chronic poorly defined. purpose this study is to determine effects canagliflozin therapy on perfusion, fibrosis, function a large model ischemia. Methods Results Yorkshire swine underwent placement an ameroid constrictor left circumflex artery...
ObjectiveLimited treatments exist for nonoperative chronic coronary artery disease. Previously, our laboratory has investigated extracellular vesicle (EV) therapy as a potential treatment disease using swine model and demonstrated improved cardiac function in treated with intramyocardial EV injection. Here, we seek to investigate the benefits of EVs by hypoxia-conditioned (HEV). Specifically, this study aims effect HEV on apoptosis chronically ischemic myocardium swine.MethodsFourteen...
Yorkshire swine were fed standard diet (
Abstract In animal models, human bone marrow mesenchymal stem cell‐derived extracellular vesicles (MSC‐EV) have been found to beneficial effects in cardiovascular disease, but only when administered via intramyocardial injection. The biodistribution of either intravenous or injection MSC‐EV the presence myocardial injury is uncharacterized at this time. We hypothesized that will ensure delivery ischemic myocardium, while not. Human cells were cultured and isolated characterized. MSC‐EVs then...
We have previously shown that normoxia serum-starved extracellular vesicle (EV) therapy improves myocardial function, perfusion, and angiogenesis in a swine model of chronic ischemia. Hypoxia-modified EVs increased abundance anti-oxidant, pro-angiogenic, pro-survival proteins. The purpose this study is to investigate the differential effects hypoxia-modified on microvascular density chronically ischemic myocardium. Yorkshire underwent placement an ameroid constrictor left circumflex artery...
Background This study aimed at evaluating the effects of JP4-039, a mitochondria-specific reactive oxygen species (mito-ROS) scavenger, on coronary angiogenesis and cardiac function in post-myocardial infarction (MI) animal model. Methods Mice underwent ligation left anterior descending (LAD) artery to induce MI received intraperitoneal (i.p.) injections JP4-039 or vehicle (n=8 animals/group) three times/week for four weeks. Echocardiography immunohistochemistry Infarction area capillary...
Abstract Dipeptidyl peptidase 4 inhibitors (DPP4i) may be cardioprotective based on several small animal and clinical studies, though randomized control trials have demonstrated limited benefit. Given these discrepant findings, the role of agents in chronic myocardial disease, particularly absence diabetes, is still poorly understood. The purpose this study was to determine effects sitagliptin, a DPP4i, perfusion microvessel density clinically relevant large model ischemia. Normoglycemic...
COVID-19 infection has spread so fast in both low- and high-income countries. In December 2019, an outbreak of a respiratory disease occurred China, later, it involved different Acute neurological insults are more likely to occur severely infected patients.We tried evaluate patients with selective criteria including, the age participants 18 older confirmed diagnosis SARS-CoV-2, developed complications post infection. An overall data 1500 were collected from primary health care departments....
Recent studies demonstrate that sodium-glucose cotransporter 2 inhibitors (SGLT2i) and dipeptidyl peptidase-4 (DPP4i), two classes of antidiabetic drugs, are cardioprotective. However, the mechanisms these benefits their comparative efficacy remain unclear. We aimed to compare effects agents on cardiac function, perfusion, microvascular density using a swine model chronic myocardial ischemia.
Reactive oxygen species (ROS) are implicated in endothelial dysfunction and cardiovascular disease. Endothelial cells (ECs) produce most ATP through glycolysis rather than oxidative phosphorylation; thus mitochondrial ROS production is lower other cell types. This makes quantification of changes EC status challenging. Here, we present an optimized protocol using mitochondrial-targeted adenovirus-based redox sensor for ratiometric specific live human coronary artery EC. For complete details...
Coronary artery disease remains a leading cause of death worldwide. Bone mesenchymal stem cell-derived extracellular vesicles (EVs) have shown promise in the setting myocardial ischemia. Furthermore, properties EVs can be modified via preconditioning progenitor cells. Previous research from our lab demonstrated significant decrease proinflammatory signaling following treatment with derived starvation human bone cells (MVM EVs) porcine model chronic However, rodent models that use hypoxia...
Sodium-glucose cotransporter-2 inhibitors (SGLT2i) are cardioprotective, and canagliflozin (CANA), an SGLT2i, has been shown to improve perfusion, AMPK signaling, oxidative stress in chronically ischemic myocardium. The aim of this study is determine the effects CANA nonischemic myocardium on coronary collateralization, stress, other molecular pathways determined by proteomic profiling. Yorkshire swine underwent placement ameroid constrictor left circumflex artery. Two weeks later, pigs...
Since the emergence of first COVID-19 case in Wuhan, virus affected several health care systems. Globally, has a transforming effect on provision. Substantial evidence was clear that global surgical services were impacted. The field neurosurgery primarily affected, and most elective surgeries suspended. There are no current reports from Egypt describe mortality outcome neurosurgical procedures context pandemic.We performed study at large tertiary center (Cairo University Hospital). It is...
Cardiovascular magnetic resonance (CMR) imaging, considered the gold standard for myocarditis diagnosis, presented conflicting results on prevalence of COVID-19-associated myocarditis.
Introduction: A plethora of pathophysiological mechanisms comes into play after ischemic stroke. They contribute to both primary and secondary insult determine the functional outcome patient. Several drug treatable molecular pathways for stroke pathogenesis have been described over decades research in cerebrovascular injury. However, none these acts independently serves as a sole determinant long-term outcome. This necessitates standardized approach that merges previous efforts reveals full...