A5102004331- Birth, Development, and Health
- Regulation of Appetite and Obesity
- Pregnancy and preeclampsia studies
- Circadian rhythm and melatonin
- Liver Disease and Transplantation
- Reproductive Biology and Fertility
- Liver Disease Diagnosis and Treatment
- Electric Power System Optimization
- Reproductive Physiology in Livestock
- Alcohol Consumption and Health Effects
- Radiopharmaceutical Chemistry and Applications
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Dietary Effects on Health
- Genetic Syndromes and Imprinting
- Proteoglycans and glycosaminoglycans research
- Electrochemical sensors and biosensors
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Molecular spectroscopy and chirality
- Thyroid and Parathyroid Surgery
- S100 Proteins and Annexins
- Pharmacological Effects and Toxicity Studies
- Gestational Diabetes Research and Management
Pontificia Universidad Católica de Chile
1944-2024
Hospital Central de la Defensa Gómez Ulla
2023
Indianapolis Zoo
2021
Sonora Institute of Technology
2020
University of Bío-Bío
2014-2016
Peptides International (United States)
2007-2015
Universidad de Los Andes, Chile
2011-2012
University of Minnesota
2004
In the adult mammal circadian system, which allows predictive adaptation to daily environmental changes, comprises peripheral oscillators in most tissues, commanded by suprachiasmatic nucleus (SCN) of hypothalamus. The external environment fetus is provided its mother. primates, maternal melatonin a candidate entrain fetal rhythms, including SCN rhythms metabolic activity. We found 90% gestation capuchin monkey expression clock genes Bmal-1, Per-2, Cry-2, and Clock SCN, adrenal, pituitary,...
This study sought to evaluate FITC-conjugated cyclic RGD peptides (FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2) as fluorescent probes for in vitro assays of integrin αvβ3/αvβ5 expression tumor tissues. FITC-RGD2, FITC-Galacto-RGD2 were prepared, their binding affinity was determined using the displacement assay against (125)I-echistatin bound U87MG glioma cells. IC50 values FITC-Galacto-RGD2, FITC-RGD2 calculated be 28 ± 8, 32 7, 89 17 nM, respectively. The followed a general trend: ∼...
Diabetes mellitus (DM) is a metabolic disorder characterized by blood glucose levels above normal limits. The impact of this disease on the population has increased in recent years. It already public health problem worldwide and one leading causes death. Recently, several proposals have been developed for better regular monitoring glucose. However, theses do not discard erroneous readings they are able to anticipate critical condition. In work, we propose an algorithm based double moving...
The aim of our in vitro experiments was to examine the role transcription factor p53 controlling basic functions ovarian cells and their response hormonal treatments. Porcine granulosa cells, transfected non-transfected with a gene construct encoding p53, were cultured ghrelin FSH (all at concentrations 0, 1, 10, or 100 ng/ml). Accumulation apoptosis-related (MAP3K5) proliferation-related (cyclin B1) substances evaluated by immunocytochemistry. secretion progesterone (P(4)), oxytocin (OT),...
This study sought to evaluate [(99m)Tc(HYNIC-Galacto-RGD2)(tricine)(TPPTS)] ((99m)Tc-Galacto-RGD2: HYNIC = 6-hydrazinonicotinyl; Galacto-RGD2 Glu[cyclo[Arg-Gly-Asp-D-Phe-Lys(SAA-PEG2-(1,2,3-triazole)-1-yl-4-methylamide)]]2 (SAA 7-amino-L-glycero-L-galacto-2,6-anhydro-7-deoxyheptanamide, and PEG2 3,6-dioxaoctanoic acid); TPPTS trisodium triphenylphosphine-3,3',3″-trisulfonate) as a new radiotracer for tumor imaging. was prepared via the copper(I)-catalyzed 1,3-dipolar azide-alkyne Huisgen...
ABSTRACT Objective To determine whether maternal plasma levels of 2‐methoxyestradiol (2‐ME) are decreased early in pregnancies that subsequently develop pre‐eclampsia (PE) and this difference could be attributed to the presence Val158Met catechol‐ O ‐methyltransferase ( COMT ) polymorphism placenta. Methods Clinical characteristics samples were collected at 11 14 weeks prospectively a cohort patients. From them, 13 PE 72 control pregnant women chosen. Plasma soluble fms‐like tyrosine kinase1...
Abstract: Formation of disulfide bonds in synthetic peptides is one the more challenging transformations to achieve peptide chemistry, view possible formation oligomeric by‐products and other side reactions, as well occasional solubility problems aqueous oxidizing media. It was shown previously that 5,5′‐dithiobis(2‐nitrobenzoic acid) (DTNB ≡ Ellman's reagent), when attached polyethylene glycol‐polystyrene (PEG‐PS TM ), controlled‐pore glass (CPG), or modified Sephadex supports, an effective...
The aim of our in vitro experiments was to study the role transcription factor STAT1 and hormone ghrelin controlling porcine ovarian function. effects treatment with (0, 1, 10, 100 ng/ml), transfection-induced overexpression STAT1, their combination on apoptosis (expression apoptosis-related peptides caspase-3, BAX anti-apoptotic peptide BCL2), proliferation proliferating cell nuclear antigene PCNA, proliferation-associated protein kinase MAPK/ERK1,2) release hormones progesterone (P(4)),...
The primate fetal adrenal reaches a large size relative to body weight followed by rapid decrease in the postnatal period. We tested hypothesis that maternal melatonin stimulates growth and prevents maturation of gland. suppressed exposing eight pregnant capuchin monkeys constant light (LL) from 63% 90% gestation (term 155 days). Three these received daily oral replacement (LL + Mel). Five mothers remaining light:dark cycle were used as controls. Fetuses delivered at gestation. absence...
Hypochlorite is a strong oxidant able to induce deleterious effects in biological systems. The goal of this work was investigate the use PGR and PYR as probes assays aimed at evaluating antioxidant activities towards hypochorite apply it plant extracts employed Chilean folk medicine. consumption evaluated from decrease visible absorbance fluorescence intensity, respectively. Total phenolic content determined by Folin Ciocalteau assay. react with hypochlorite different kinetics, being...
LY3305677 (LY) is a synthetic peptide analog of mammalian oxyntomodulin with fatty acyl side chain to extend time action. LY functions are mediated by binding and activation both glucagon (GcgR) glucagon-like peptide-1 (GLP-1R) receptors. bound potent affinity (Ki) human mouse GcgR (Ki: 17.7 nM 15.9 nM, respectively) GLP-1R 28.6 25.1 respectively). dose-dependently stimulated insulin secretion in pancreatic islets (EC50: 5.2 nM) mice. In oral glucose tolerance tests diet-induced obese (DIO)...
Mining investment decisions for giant mining projects involve an enormous financial risk. Nevertheless, traditional risk evaluation methods are based on Monte Carlo simulation of cash flows and little value management. In most project scenarios, the main sources those related to ore body, specially grade reserves. this study, a new management methodology conditional is developed quantify associated with uncertainty body. The allows quantifying each period in production plan and, therefore,...
ResumenAntecedentes: Recientemente hemos propuesto en un modelo experimental de infarto al miocardio una significativa interregulación entre los niveles la enzima convertidora angiotensina I (ECA) y su homóloga (ECA-2), junto con que (Ang)-(1-9) más Ang-(1-7) actuaría como contrarregulador Ang II.Sin embargo tal relación no se ha investigado el remodelado aórtico hipertensivo.Objetivo: Determinar expresión ECA ECA-2, Angs I, II, (1-7) (1-9) parámetros pared aórtica ratas hipertensas.Métodos:...
The title compound, C(23)H(26)F(2)N(2)O(4), is a dipeptidic inhibitor of γ-secretase, one the enzymes involved in Alzheimer's disease. molecule adopts compact conformation, without intramolecular hydrogen bonds. In crystal structure, amide N atoms forms only intermolecular N-H...O bond; second atom does not form High-resolution synchrotron diffraction data permitted unequivocal location and refinement restraints all H atoms, identification characteristic shift engaged bond from its ideal...