A5019953042- Cystic Fibrosis Research Advances
- HIV/AIDS Research and Interventions
- Pancreatitis Pathology and Treatment
- Neonatal Respiratory Health Research
- HIV Research and Treatment
- Healthcare Policy and Management
- Reproductive tract infections research
- Gastrointestinal disorders and treatments
- HIV, Drug Use, Sexual Risk
- LGBTQ Health, Identity, and Policy
- Ion channel regulation and function
- Distributed systems and fault tolerance
- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- Formal Methods in Verification
- Ion Transport and Channel Regulation
- Patient-Provider Communication in Healthcare
- Pediatric Hepatobiliary Diseases and Treatments
- Adolescent Sexual and Reproductive Health
- Petri Nets in System Modeling
- Cannabis and Cannabinoid Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Ethics and Social Impacts of AI
- Topological and Geometric Data Analysis
- Advanced biosensing and bioanalysis techniques
Wayne State University
2012-2024
University of Alberta
2016-2023
Japan Society
2023
Duke Medical Center
1999-2022
Durham VA Medical Center
2000-2022
University of Puerto Rico at Río Piedras
2016
Public Citizen
2016
Milbank Memorial Fund
2016
Yale University
1987-2015
Oregon Health & Science University
2013
Cystic fibrosis transmembrane regulator (CFTR), the gene product that is mutated in cystic (CF) patients, has a well-recognized function as cyclic adenosine 3′,5′-monophosphate (cAMP)-regulated chloride channel, but this property does not account for abnormally high basal rate and cAMP sensitivity of sodium ion absorption CF airway epithelia. Expression complementary DNAs rat epithelial Na + channel (rENaC) alone Madin Darby canine kidney (MDCK) cells generated large amiloride-sensitive...
It is unknown whether genetic factors predispose patients to idiopathic pancreatitis. In with cystic fibrosis, mutations of the fibrosis transmembrane conductance regulator (CFTR ) gene typically cause pulmonary and pancreatic insufficiency while rarely causing We examined pancreatitis associated CFTR in persons who do not have lung disease fibrosis.
<h3>PURPOSE</h3> Mindfulness (ie, purposeful and nonjudgmental attentiveness to one's own experience, thoughts, feelings) is associated with physician well-being. We sought assess whether clinician self-rated mindfulness the quality of patient care. <h3>METHODS</h3> conducted an observational study 45 clinicians (34 physicians, 8 nurse practitioners, 3 assistants) caring for patients infected human immunodeficiency virus (HIV) who completed Mindful Attention Awareness Scale 437 HIV-infected...
Regulation of epithelial chloride flux, which is defective in patients with cystic fibrosis, may be mediated by phosphorylation the fibrosis transmembrane conductance regulator (CFTR) cyclic AMP-dependent protein kinase (PKA) or C (PKC). Part R-domain CFTR (termed CF-2) was expressed and purified from Escherichia coli. CF-2 phosphorylated on seryl residues PKA, PKC, GMP-dependent (PKG), calcium/calmodulin-dependent I (CaM I). Direct amino acid sequencing peptide mapping revealed that serines...
Cystic fibrosis (CF) is characterized by an abnormality in cAMP-regulated chloride transport that results from a primary defect the protein product of CF gene, transmembrane conductance regulator (CFTR). In this report, antibodies against CFTR peptides were used to localize human pancreas. An affinity purified antibody (alpha-1468) raised synthetic peptide identified 155-170-kD on immunoblot. Cytochemical studies with alpha-1468 localized small branching, tubular structures. The same...
Critical to an understanding of the pulmonary disease in cystic fibrosis (CF) and development effective gene therapies is a definition distribution regulation CF expression adult human lung. Previous studies have detected product gene, transmembrane conductance regulator (CFTR), submucosal glands bronchi. In this report, we characterized CFTR RNA protein distal airway alveoli lungs. Samples from eight lungs were analyzed for by situ hybridization immunocytochemistry. was subpopulation...
<h3>PURPOSE</h3> Health care leaders encourage clinicians to offer portals that enable patients access personal health records, but implementation has been a challenge. Although large integrated systems have promoted use through costly advertising campaigns, other methods are needed for small medium-sized practices where most receive their care. <h3>METHODS</h3> We conducted mixed assessment of proactive strategy patient portal (an interactive preventive record [IPHR]) offered by 8 primary...
The CFTR splicing mutation 3849 + 10 kb C --> T creates a novel donor site kilobases (kb) into intron 19 of the gene and is one more common mutations that causes cystic fibrosis (CF). It has an elevated prevalence among patients with atypically mild disease normal sweat electrolytes especially prominent in Ashkenazi Jews. This class mutations, reported several genes, involves splice sites activated deep within introns while leaving wild-type elements intact. cDNA constructs modeled were...
The rat liver mixed function oxidase system which is responsible for the metabolism of endogenous and exogenous compounds has been shown to be affected by lead methyl mercury. Administration these environmental pollutants rats results in a decrease cytochrome P-450 content inhibition vitro N-demethylase hydroxylase activities. enzyme-inhibiting effects metals found pharmacological expression whole animal prolongation hexobarbital-induced sleeping times.
Cystic fibrosis results from mutations in the gene encoding CFTR Cl- channel. Although occurs as an integral component of plasma membrane, recent studies implicate endocytic recycling and suggest that protein may also exist intracellular vesicular compartments. To test this, we analyzed clathrin-coated vesicles (CCV) purified cells constitutively expressing at high levels. immunoreactivity was detected CCV by immunoblot identified based on labeling immunoprecipitates with kinase A tryptic...
We previously proposed that ductal bile formation is regulated by secretin-responsive relocation of aquaporin 1 (AQP1), a water-selective channel protein, from an intracellular vesicular compartment to the apical membrane cholangiocytes. In this study, we immunoisolated AQP1-containing vesicles cholangiocytes prepared rat liver; quantitative immunoblotting revealed enrichment in these not only AQP1 but also cystic fibrosis transmembrane regulator (CFTR) and AE2, Cl- Cl−/HCO3− exchanger,...
Objective To assess the variation in HIV-1 over menstrual cycle, including RNA levels female genital tract, plasma HIV-1-RNA levels, CD4 cell counts, and culturable virus. Design A prospective analysis of 55 HIV-1-infected women. Methods Blood tract specimens were collected weekly 8 weeks, spanning two complete cycles. Applying repeated-measures models that used menses as reference level, viral was compared endocervical canal fluid cells (collected by Sno-strips cytobrush, respectively)...
Cystic fibrosis (CF) is a recessive disease caused by mutations of the CF transmembrane conductance regulator (CFTR) gene. The risk idiopathic chronic pancreatitis (ICP) increased in individuals who have CFTR genotypes containing CF-causing mutation plus second pathogenic allele. It unknown whether ICP carriers one normal In this study, 52 sporadic cases were ascertained through European Registry Hereditary Pancreatitis and Familial Pancreatic Cancer. Individuals with cationic trypsinogen...
Disruption of the renal proximal tubule (PT) brush border is a prominent early event during ischemic injury to kidney. The molecular basis for this unknown. Within border, ezrin may normally link cytoskeleton cell plasma membrane. Anoxia causes dissociate from and also many proteins become dephosphorylated in PTs. This study examines hypothesis that dephosphorylation accompanies mediate anoxic disruption rabbit PT. During normoxia, 73 +/- 3% cytoskeleton-associated (Triton-insoluble) was...