A5049018710- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Ophthalmology and Visual Impairment Studies
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Health and Medical Research Impacts
- Intraocular Surgery and Lenses
- Health Sciences Research and Education
- Herpesvirus Infections and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Ocular Disorders and Treatments
- Signaling Pathways in Disease
- History of Medical Practice
- Cellular Mechanics and Interactions
- Retinal Imaging and Analysis
- Ocular Diseases and Behçet’s Syndrome
- Ocular Oncology and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Myasthenia Gravis and Thymoma
- Otitis Media and Relapsing Polychondritis
- Spinal Cord Injury Research
- Social Media in Health Education
Sunnybrook Health Science Centre
2021-2025
University of Toronto
2016-2025
Kensington Health
2020-2025
Sunnybrook Hospital
2023-2025
Health Sciences Centre
2021-2025
Sunnybrook Research Institute
2021-2024
Harvard University
2019-2023
Massachusetts Eye and Ear Infirmary
2019-2023
Cohen Children's Medical Center
2023
Johnson & Johnson (Netherlands)
2022
Fuchs endothelial corneal dystrophy (FECD) is a leading cause of (CE) degeneration resulting in impaired visual acuity. It genetically complex and age-related disorder, with higher incidence females. In this study, we established nongenetic FECD animal model based on the physiologic outcome CE susceptibility to oxidative stress by demonstrating that exposure ultraviolet A (UVA) recapitulates morphological molecular changes FECD. Targeted irradiation mouse corneas UVA induced reactive oxygen...
Myelin-associated inhibitors (MAIs) contribute to failed regeneration in the CNS. The intracellular signaling pathways through which MAIs block axonal repair remain largely unknown. Here, we report that kinase GSK3β is directly phosphorylated and inactivated by MAIs, consequently regulating protein–protein interactions are critical for myelin-dependent inhibition. Inhibition of mimics neurite outgrowth inhibitory effect myelin. effects myelin not additive indicating a major effector MAIs....
To treat unilateral limbal stem cell (LSC) deficiency, we developed cultivated autologous epithelial cells (CALEC) using an innovative xenobiotic-free, serum-free, antibiotic-free, two-step manufacturing process for LSC isolation and expansion onto human amniotic membrane with rigorous quality control in a good practices facility. Limbal biopsies were used to generate CALEC constructs, final grafts evaluated by noninvasive scanning microscopy tested viability sterility. Cultivated maintained...
Myelin-associated inhibitors (MAIs) and chondroitin sulfate proteoglycans (CSPGs) contribute to failed regeneration after neuronal injury. MAIs CSPGs stimulate intracellular signals including the activation of RhoA Rho kinase block axonal extension through targeted modifications cytoskeleton. ROCK are promising targets for therapeutic intervention promote CNS repair; however, their ubiquitous expression will limit specificity drugs these molecules. We have identified cytosolic phosphoprotein...
To compare accelerated corneal crosslinking (CXL) alone, CXL with simultaneous intrastromal ring segments (CXL-ICRS), and topography-guided photorefractive keratectomy (CXL-TG-PRK) in progressive keratoconus, pellucid marginal degeneration (PMD), or laser situ keratomileusis (LASIK)-induced ectasia.The Kensington Eye Institute Bochner Institute, Toronto, Canada.Prospective nonrandomized interventional study.Visual topographical outcomes using a comparative analysis adjusting for preoperative...
To develop an algorithm to classify multiple retinal pathologies accurately and reliably from fundus photographs validate its performance against human experts.
Repair of the injured spinal cord is a major challenge in medicine. The limited intrinsic regenerative response mounted by adult central nervous system (CNS) neurons further hampered astrogliosis, myelin debris and scar tissue that characterize damaged CNS. Improved axon regeneration recovery can be elicited targeting extrinsic factors as well boosting neuron-intrinsic growth regulators. Our knowledge molecular basis regulators has expanded rapidly, resulting promising new targets to promote...
Abstract Fuchs endothelial corneal dystrophy (FECD) is a complex disease characterized by the progressive decline and morphological changes of cells (CECs) that leads to edema vision loss. The most common mutation in FECD an intronic CTG repeat expansion transcription factor 4 ( TCF4 ) its altered expression. Corneal wound healing occurs primarily through cell enlargement migration, CECs have been shown display increased migration speeds. In this study, we aim determine whether can promote...
Aims This study aims to investigate whether denoising diffusion probabilistic models (DDPMs) could generate realistic retinal images, and if they be used improve the performance of a deep convolutional neural network (CNN) ensemble for multiple disease classification, which was previously shown outperform human experts. Methods We trained DDPMs fundus images representing diabetic retinopathy, age-related macular degeneration, glaucoma or normal eyes. Eight board-certified ophthalmologists...
Background Glycogen Synthase Kinase 3 (GSK3) has been implicated in regulating chromosomal alignment and mitotic progression but the physiological substrates mediating these GSK3-dependent effects have not identified. Collapsin Response Mediator Protein 4 (CRMP4) is a cytosolic phosphoprotein known to regulate cytoskeletal dynamics substrate of GSK3. In this study, we investigate role CRMP4 during mitosis. Methodology Principal Findings Here demonstrate that mitosis phosphorylation regulated...
To compare the symptoms and signs of dry eye disease (DED) in children with systemic lupus erythematosus (SLE) those healthy using common diagnostic tools.Prospective, observational, single-center cohort study. Thirty-four subjects SLE 15 were recruited from Hospital for Sick Children Toronto, Canada. Subjects underwent subjective objective assessments Canadian Dry Eye Assessment (CDEA) questionnaire, tear film osmolarity, slit lamp examination, break-up time, corneal fluorescein staining,...
Purpose: To compare the one-year outcomes of preloaded Descemet membrane endothelial keratoplasty (pDMEK) and non-preloaded DMEK (n-pDMEK) in patients with Fuchs corneal dystrophy (FECD). Methods: This retrospective comparative cohort study consecutively included 68 eyes who underwent either pDMEK (n = 38) or n-pDMEK 30) performed by cornea fellows an experienced surgeon between 2016 2018 at Massachusetts Eye Ear Infirmary. Exclusion criteria were previous surgery (other than uncomplicated...
Aims To determine the cost-effectiveness of preloaded Descemet membrane endothelial keratoplasty (pDMEK) versus non-preloaded DMEK (n-pDMEK) for treatment Fuchs corneal dystrophy (FECD). Methods From a societal and healthcare perspective, this retrospective analysis analysed cohort 58 patients with FECD receiving pDMEK (n=38) or n-pDMEK (n=30) from 2016 to 2018 in Department Ophthalmology, Massachusetts Eye Ear Infirmary, Harvard Medical School, Boston, USA. Exclusion criteria were previous...
PurposeTo investigate if corneal endothelial cells (CECs) in Fuchs dystrophy (FECD) have altered cellular migration compared with normal controls.DesignComparative analysis.MaterialsDescemet's membrane and CECs derived from patients FECD undergoing keratoplasty or cadaveric donors.MethodsEx vivo specimens were used for live cell imaging generation of immortalized lines. Live was performed on ex transfected green fluorescent protein. Migration speeds determined as a function density using...
Abstract Purpose To evaluate the relationship between subjective (slit lamp examination [SLE]) and objective (densitometry) measurements of corneal haze after accelerated crosslinking (aCXL), assess densitometry corrected distance visual acuity (CDVA), determine effect baseline characteristics on aCXL in eyes with progressive keratoconus other ectasias. Setting Kensington Eye Institute Bochner Institute, Toronto, Canada. Design Retrospective analysis a prospective interventional cohort...