A5008134383- Tuberculosis Research and Epidemiology
- Orthopedic Surgery and Rehabilitation
- Nerve Injury and Rehabilitation
- Mycobacterium research and diagnosis
- Peripheral Nerve Disorders
- Congenital limb and hand anomalies
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Elbow and Forearm Trauma Treatment
- Pulmonary Hypertension Research and Treatments
- Immune cells in cancer
- Botulinum Toxin and Related Neurological Disorders
- Renin-Angiotensin System Studies
- Chemokine receptors and signaling
- Blood disorders and treatments
- Nitric Oxide and Endothelin Effects
- Dental Research and COVID-19
- Infectious Diseases and Tuberculosis
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Structural Anomalies and Repair
- Viral gastroenteritis research and epidemiology
- Diversity and Career in Medicine
- Health and Medical Education
- Musculoskeletal pain and rehabilitation
Pontificia Universidad Católica de Chile
2016-2025
Texas Biomedical Research Institute
2020-2025
Mayo Clinic
2024
Mayo Clinic in Arizona
2023
Instituto Profesional Carlos Casanueva
2022
Shriners Hospitals for Children - Philadelphia
2021
Cornell University
2018-2019
The University of Texas at San Antonio
2011-2017
Institut Català d'Oncologia
2015
The University of Texas Health Science Center at Houston
2008-2013
Vaccine and antiviral development against SARS-CoV-2 infection or COVID-19 disease would benefit from validated small animal models. Here, we show that transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) by the cytokeratin 18 promoter (K18 hACE2) represent a susceptible rodent model. K18 hACE2 succumbed to day 6, with virus detected in lung airway epithelium brain. ACE2 produced modest TH1/2/17 cytokine storm spleen peaked 2, an extended chemokine was both lungs This...
Abstract Microglial cells are difficult to track during development because of the lack specific reagents for myeloid subpopulations. To further understand how lineages differentiate create microglial cells, we investigated CX3CR1 and CCR2 transcription unit activation in Cx3cr1+/GFPCCR2+/RFP knockin fluorescent protein reporter mice. The principal findings include: 1) CX3CR1+ localized aorta–gonad–mesonephros region, visualized at embryonic day (E)9.0 yolk sac neuroectoderm; 2) E10.5,...
Background. Although the biological basis for increased susceptibility of diabetic patients to tuberculosis remains unclear, world is undergoing a type 2 diabetes pandemic. We hypothesize that chronic hyperglycemia leads immunocompromise facilitates progression active tuberculosis. To assess this possibility, we determined whether with and (particularly those hyperglycemia), compared who did not have diabetes, presented altered cytokine responses mycobacterial antigen. Methods. Samples whole...
Isolation of immune cells that infiltrate the central nervous system (CNS) during infection, trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and effector functions. Histochemical approaches are instrumental determine location infiltrating analyze associated CNS pathology. However, in-situ histochemistry immunofluorescent staining techniques limited by number antibodies can be used at a single time characterize cell subtypes in particular tissue....
Abstract Fractalkine, a chemokine anchored to neurons or peripheral endothelial cells, serves as an adhesion molecule soluble chemoattractant. Fractalkine binds CX3CR1 on microglia and circulating monocytes, dendritic NK cells. The aim of this study is determine the role in trafficking function myeloid cells CNS during experimental autoimmune encephalomyelitis (EAE). Our results show that, models active EAE, Cx3cr1−/− mice exhibited more severe neurologic deficiencies. Bone marrow chimeric...
The elderly population is highly susceptible to developing respiratory diseases, including tuberculosis, a devastating disease caused by the airborne pathogen Mycobacterium tuberculosis (M.tb) that kills one person every 18 seconds. Once M.tb reaches alveolar space, it contacts lining fluid (ALF), which dictates host-cell interactions. We previously determined age-associated dysfunction of soluble innate components in human ALF leads accelerated growth within macrophages. Here we impact on...
To describe early functional outcomes of nerve transfer surgery in a relatively large cohort patients with acute flaccid myelitis (AFM).A retrospective case analysis was made AFM treated between 2007 and 2018. Surgical criteria were persistent motor deficits after 6 months from onset available donor nerves. Thirty-two evaluated; 16 underwent surgeries. Motor function evaluated by licensed occupational therapist using the Active Movement Scale preoperatively during follow-up examinations....
Isolation of immune cells that infiltrate the central nervous system (CNS) during infection, trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and effector functions. Histochemical approaches are instrumental determine location infiltrating analyze associated CNS pathology. However, in-situ histochemistry immunofluorescent staining techniques limited by number antibodies can be used at a single time characterize cell subtypes in particular tissue....
Abstract Background This study aimed to evaluate if access orthopedic surgery differs by healthcare coverage in a country with dual system adjusted age, sex, and urgent elective conditions. We hypothesize that differential would exist according the type of coverage. difference accentuate when analyzing surgery. Methods A cross-sectional, population-based design was used investigate rates Chile 2018. The surgeries provided under private public systems were calculated per 1,000 inhabitants...
ABSTRACT There is currently a public health crisis due to the rise of multi-drug-resistant tuberculosis cases, as well in number deaths from tuberculosis. To achieve United Nations Sustainable Development Goal ending epidemic by 2030, new treatments are urgently required. We previously reported discovery 49 , pre-clinical candidate that acted through inhibition Mycobacterium lysyl-tRNA synthetase (LysRS). In this report, full medicinal chemistry program reviewed original hit optimised lead....
The development of an accurate antigen detection assay for the diagnosis active tuberculosis (TB) would represent a major clinical advance. Here, we demonstrate that Mycobacterium Rv1681 protein is biomarker TB with potential diagnostic utility. We initially identified, by mass spectroscopy, peptides from in urine specimens 4 patients untreated TB. Rabbit IgG anti-recombinant detected lysates and culture filtrates M. immunoprecipitated it pooled two patients. An enzyme-linked immunosorbent...
Although angiotensin II (ANG II) is a known pulmonary vasoconstrictor, the purpose of this study was to examine effect ANG on artery endothelial cell nitric oxide synthase (ecNOS) mRNA and protein expression. Cultured bovine (BPAE; passages 5-8) cells were incubated for 0-12 h with 10(-6) M II. Total RNA extracted, ecNOS expression assessed by Northern blot analysis. In BPAE cells, significantly increased 2.4 +/- 0.3-fold (P < 0.05 vs. basal; n = 5) 6 after addition over basal levels....
The precise microenvironment of Paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are sensitive enough. We applied to this purpose polymerase chain reaction (PCR) using three sets specific primers corresponding two P. genes. This fungus as well several other fungi, were grown and their DNA obtained by mechanical disruption a phenol chloroform isoamylalcohol-based purification method. served for PCR that employed from genes codify antigenic proteins,...
Conventionally, hyperimmune globulin drugs manufactured from pooled immunoglobulins vaccinated or convalescent donors have been used in treating infections where no treatment is available. This especially important multi-epitope neutralization required to prevent the development of immune-evading viral mutants that can emerge upon with monoclonal antibodies. Using microfluidics, flow sorting, and a targeted integration cell line, first-in-class recombinant therapeutic against SARS-CoV-2...
Diabetes knowledge among TB patients can contribute to improved treatment outcomes, but lack of diabetes diagnosis awareness is a limitation in developing countries. Given its low cost, the sensitivity urine glucose dipsticks for screening was assessed. Glycosuria assessed 90 newly diagnosed (38 with diabetes) south Texas, USA (n = 20) and northeast Mexico 70) during January 2009–December 2010. detected 65% diabetic chronic hyperglycemia (positive predictive value 91%, negative 84%). We...
Microbiological identification of Mycobacterium tuberculosis is insensitive and slow, clinical distinction tuberculous meningitis (TBM) from other subacute or chronic meningoenchephalitides (SACM) difficult. Successful use highly specific M. serological assays on cerebrospinal fluid has been reported, but their performance for diagnosis in a endemic country where they would be most value unclear. We sought to determine the biological basis uncertainty interpretation antibody detection CSF...