A5030776801- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Vector-borne infectious diseases
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Phosphodiesterase function and regulation
- Immune cells in cancer
- Plant Stress Responses and Tolerance
- Essential Oils and Antimicrobial Activity
- Retinal Development and Disorders
- Identification and Quantification in Food
- Plant responses to water stress
- RNA modifications and cancer
- Immunodeficiency and Autoimmune Disorders
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- Advanced Chemical Sensor Technologies
- Tannin, Tannase and Anticancer Activities
- Autophagy in Disease and Therapy
- Cultural Heritage Materials Analysis
- Planarian Biology and Electrostimulation
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Pluripotent Stem Cells Research
University of Oxford
2018-2025
Oxford Research Group
2020-2023
Universidade Nova de Lisboa
2012-2015
Universidade Portucalense
2012-2015
Hôpital Necker-Enfants Malades
2015
Assistance Publique – Hôpitaux de Paris
2015
Instituto Português Da Qualidade
2006-2014
Instituto de Tecnología Química
2013
University of Lisbon
2003
Protein modification has entered the limelight of chemical and biological sciences, since, by appending small molecules into proteins surfaces, fundamental biophysical processes may be studied even modulated in a physiological context. Herein we present new strategy to modify lysine's ε-amino group protein's N-terminal, based on formation stable iminoboronates aqueous media. This functionality enables complete these amine groups, which can reversible upon addition fructose, dopamine, or...
Predatory bacteria, like the model endoperiplasmic bacterium Bdellovibrio bacteriovorus, show several adaptations relevant to their requirements for locating, entering and killing other bacteria. The mechanisms underlying prey recognition handling remain obscure. Here we use complementary genetic, microscopic structural methods address this deficit. During invasion, B. bacteriovorus protein CpoB concentrates into a vesicular compartment that is deposited periplasm. Proteomic analyses of...
Abstract Altered central carbon metabolism is a hallmark of many diseases including diabetes, obesity, heart disease and cancer. Identifying metabolic changes will open opportunities for better understanding aetiological processes identifying new diagnostic, prognostic, therapeutic targets. Comprehensive robust analysis primary pathways in cells, tissues bio-fluids, remains technically challenging. We report on the development validation highly reproducible untargeted method using...
The use of artemisinin or other endoperoxides in combination with drugs is a strategy to prevent development resistant strains Plasmodium parasites. Our previous work demonstrated that hybrid compounds, comprising and vinyl sulfones, were capable high activity profiles comparable chloroquine while acting through two distinct mechanisms action: oxidative stress falcipain inhibition. In this study, we adapted approach novel class inhibitors: peptidomimetic pyrimidine nitriles. Pyrimidine...
Significance The plant cysteine oxidases (PCOs) have been identified as oxygen-sensing enzymes in plants, controlling hypoxia-dependent processes, including adaptive responses to flooding. As such, they are potential targets for engineering plants with enhanced flood tolerance. To approach this a rational manner requires an understanding of how PCO structure relates their ability trigger hypoxic adaptation. We report the structures two PCOs from Arabidopsis and show that effects mutagenesis...
Human induced pluripotent stem cells (iPSCs) and macrophages derived from them are increasingly popular tools for research into both infectious degenerative diseases. However, as the field strives greater modeling accuracy, it is becoming ever more challenging to justify use of undefined proprietary media culture these cells. Here, we describe a defined, serum-free, open-source medium differentiation iPSC-derived macrophages. This equally capable maintaining compared with commercial...
Abstract Androgen deprivation therapy (ADT) is the standard care for prostate cancer (PCa) patients who fail surgery or radiotherapy. While initially effective, almost always recurs as a more aggressive castration resistant (CRPC). Previous studies have demonstrated that chromatin modifying enzymes can play critical role in conversion to CRPC. However, only handful of these potential pharmacological targets been tested. Therefore, this study, we conducted focused shRNA screen previously...
JmjC domain-containing protein 6 (JMJD6) is a 2-oxoglutarate (2OG)-dependent oxygenase linked to various cellular processes, including splicing regulation, histone modification, transcriptional pause release, hypoxia sensing, and cancer. JMJD6 reported catalyze hydroxylation of lysine residue(s) histones, the tumor-suppressor p53, regulatory proteins, u2 small nuclear ribonucleoprotein auxiliary factor 65-kDa subunit (U2AF65). also N-demethylation N-methylated (both mono- di-methylated)...
Abstract Propiogenic substrates and gut bacteria produce propionate, a post-translational protein modifier. In this study, we used mouse model of propionic acidaemia (PA) to study how disturbances propionate metabolism result in histone modifications changes gene expression that affect cardiac function. Plasma surrogates were raised PA mice, but female hearts manifested more profound acyl-CoAs, propionylation acetylation, transcription. These resulted moderate diastolic dysfunction with Ca...
Barnacle adhesion underwater is an important phenomenon to understand for the prevention of biofouling and potential biotechnological innovations, yet so far, identifying what makes barnacle glue proteins 'sticky' has proved elusive. Examination a broad range species within barnacles may be instructive identify conserved adhesive domains. We add extensive information from acorn (order Sessilia) by providing first protein analysis stalked adhesive, Lepas anatifera Lepadiformes). It was...
Tropical forests constitute a prolific sanctuary of unique floral diversity and potential medicinal sources, however, many them remain unexplored. The scarcity rigorous scientific data on the surviving Mascarene endemic taxa renders bioprospecting this untapped resource utmost importance. Thus, in view valorizing native resource, study has as its objective to investigate bioactivities leaf extracts. Herein, seven plants leaves were extracted evaluated for their vitro antioxidant properties...
All aerobic organisms require O2 for survival. When their is limited (hypoxia), a response required to reduce demand and/or improve supply. A hypoxic mechanism has been identified in flowering plants: the stability of certain proteins with N-terminal cysteine residues regulated an O2-dependent manner by Cys/Arg branch N-degron pathway. These include Group VII ethylene factors (ERF-VIIs), which can initiate adaptive responses hypoxia. Oxidation catalyzed plant oxidases (PCOs), destabilizing...
Silk has been a luxurious commodity throughout modern human history and sericulture played an important role in ancient global trade as well technological cultural developments. Archaeological findings suggest that prior to domestication of the mulberry silkworm (Bombyx mori) silks were obtained from range silk-producing moth species with regional specificity. However, investigating origins is difficult classification by species-type proved technically challenging. We therefore investigated...
Abstract Channelling of glucose via glycogen, known as the glycogen shunt, may play an important role in metabolism brain tumours, especially hypoxic conditions. We aimed to dissect degradation glioblastoma (GBM) response ionising radiation (IR). Knockdown phosphorylase liver isoform (PYGL), but not (PYGB), decreased clonogenic growth and survival GBM cell lines sensitised them IR doses 10–12 Gy. Two five days after exposure PYGL knockdown cells, mitotic catastrophy a giant multinucleated...
The use of Moloney murine leukaemia virus (MoMLV) derived retroviral vectors in gene therapy requires the production high titer preparations. However, obtaining titers infective MoMLV is difficult due to vector inherent instability. In this work effect cell culture medium osmotic pressure upon stability was studied. osmolality standard raised from 335 up 500 mOsm/kg using either ionic (sodium chloride) or non-ionic agents (sorbitol and fructose). It observed that, independently agent used,...