A5083639931- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Ocular Surface and Contact Lens
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Cellular Mechanics and Interactions
- Salivary Gland Disorders and Functions
- Ion Transport and Channel Regulation
- Drug Transport and Resistance Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Protease and Inhibitor Mechanisms
- Caveolin-1 and cellular processes
- Connective tissue disorders research
- Botulinum Toxin and Related Neurological Disorders
- Monoclonal and Polyclonal Antibodies Research
- Bone and Dental Protein Studies
- Ophthalmology and Eye Disorders
- RNA Interference and Gene Delivery
- Nasolacrimal Duct Obstruction Treatments
- Galectins and Cancer Biology
- Polyamine Metabolism and Applications
- Heat shock proteins research
- Restless Legs Syndrome Research
University of Southern California
2014-2024
Keck Hospital of USC
2022-2023
LAC+USC Medical Center
2003-2020
Southern California University for Professional Studies
2008-2020
Smith-Kettlewell Eye Research Institute
2005
Pacific Northwest Diabetes Research Institute
2005
Pharmaceutical Biotechnology (Czechia)
2005
Augusta University
2002
Johannes Gutenberg University Mainz
1999
University of California, San Francisco
1992-1994
Sjögren's syndrome is a chronic autoimmune disease affecting the lacrimal glands and other epithelia. It has been suggested that acinar cells of provoke local responses, leading to when they begin expressing major histocompatibility complex (MHC) class II molecules. We used isopycnic centrifugation phase partitioning resolve compartments participate in traffic between basolateral membranes endomembrane system test hypothesis MHC molecules enter contain potential autoantigens, i.e., La/SSB,...
Lasp-1 has been identified as a signaling molecule that is phosphorylated upon elevation of [cAMP]i in pancreas, intestine and gastric mucosa selectively expressed cells within epithelial tissues. In the parietal cell, cAMP-dependent phosphorylation induces partial translocation lasp-1 to apically directed F-actin-rich canalicular membrane, which site active HCl secretion. an unusual modular protein contains N-terminal LIM domain, C-terminal SH3 domain two internal nebulin repeats....
Aim: Secretion of proteins into basal tears Parkinson's disease (PD) patients may be altered by changes in nerve function. Materials & methods: Oligomeric α-SynOligo and total α-SynTotal, CCL-2, DJ-1, LF MMP-9 were measured from 93 PD 82 age- sex-equivalent healthy controls. Results: α-SynTotal was decreased (p = 0.0043), whereas < 0.0001) the ratio α-SynOligo/α-SynTotal increased compared with Area under receiver-operating curves contents 0.70 (95% confidence limits: 0.621–0.774) 0.72...
The C-terminal cytoplasmic domain of the polymeric immunoglobulin receptor (pIgR) contains two tyrosine residues, Tyraes and Tyr734.Previous work identifying T Y ~' ' ~ as a critical residue in endocytosis pIgR Madin-Darby canine kidney (MDCK) cells also suggested that second functional internalization signal was present (Breitfeld, P.
We have isolated cDNA clones encoding the bovine and rat gastric H,K-ATPase beta subunit. A abomasum lambda gt11 library was screened with a monoclonal antibody raised against rabbit single positive phage clone containing an approximately 900-base pair insert identified as reactive antibody. The identity of established by comparing deduced amino acid sequence sequences cyanogen bromide fragments porcine Polymerase chain reaction rapid amplification ends were used to generate fragment...
Molecular events defining enamel matrix removal during amelogenesis are poorly understood. Early reports have suggested that adaptor proteins (AP) participate in ameloblast-mediated endocytosis. Enamel formation involves the secretory and maturation stages, with an increase resorptive function latter. Here, using real-time PCR, we show expression of clathrin protein subunits upregulated stage rodent organ cells. AP complex 2 (AP-2) is most four distinct complexes. Immunolocalization confirms...
Aim: Due to active engagement of sensory and afferent nerve fibers in reflex tearing which could be affected Parkinson's disease (PD), we tested tears as a source potential PD biomarkers. Patients & methods: Reflex collected from 84 age- sex-equivalent healthy controls (HC) were used measure levels oligomeric α-Syn (α-SynOligo), total (α-SynTotal), CCL2, DJ-1, lactoferrin MMP9. Results: α-synOligo (p < 0.0001), CCL2 = 0.003) 0.002) significantly elevated patient relative HC tears. Tear flow...
Subconfluent cultures of Madin-Darby canine kidney (MDCK) and CV-1 cells were immunostained with two monoclonal antibodies (MAbs), MAb X-22 23, against clathrin heavy chain polyclonal antiserum a conserved region all mammalian light chains. In interphase MDCK cells, staining by three resulted in the characteristic intracellular punctate vesicular perinuclear pattern. mitotic anti-clathrin strongly stained spindle. Staining spindle was colocalized anti-tubulin microtubular arrays evident from...
5726 of the cytoplasmic domain polymeric immunoglobulin receptor (pIgR) resides within a consensus sequence for phosphorylation by protein kinases A, G, and C, casein kinase 11.Mutation to Ala expression this mutant pIgR in Madin-Darby canine kidney cells results which steadystate is reduced 49% wild-type levels.This also significantly impaired its internalization from basolateral membrane.During first minute, radioiodinated ligands (either dIgA or monovalent anti-pIgR Fabs) only 35% that...
In this article, we investigate the contributions of actin filaments and accessory proteins to apical clathrin-mediated endocytosis in primary rabbit lacrimal acini. Confocal fluorescence electron microscopy revealed that cytochalasin D promoted accumulation clathrin, alpha-adaptin, dynamin, F-actin increased amounts coated pits vesicles at plasma membrane. Sorbitol density gradient analysis membrane compartments showed [14C]dextran association with membranes from stimulated acini,...
The enamel matrix protein amelogenin is secreted by ameloblasts into the extracellular space to guide formation of highly ordered hydroxyapatite mineral crystallites, and, subsequently, almost completely removed during maturation. Amelogenin interacts with transmembrane proteins CD63 and LAMP (lysosome-associated membrane protein) 1, which are involved in endocytosis. Exogenously added has been observed move rapidly CD63/LAMP1-positive vesicles cultured cells. In present study, we...
The mechanism responsible for the altered spectrum of tear proteins secreted by lacrimal gland acinar cells (LGAC) in patients with Sjögren's Syndrome (SS) remains unknown. We have previously identified increased cathepsin S (CTSS) activity as a unique characteristic tears SS. Here, we investigated role Rab3D, Rab27a, and Rab27b enhanced release CTSS from LGAC. Similar to SS male nonobese diabetic (NOD) mouse model SS, was elevated mice lacking Rab3D. Findings lower gene expression...
γ-Adaptin and clathrin heavy chain were identified on tubulovesicles of gastric oxyntic cells with the anti-γ-adaptin monoclonal antibody (MAb) 100/3 an anti-clathrin MAb (MAb 23), respectively. In Western blots, crude microsomes from rabbit rat density gradient-purified, H-K-ATPase-rich these same species immunoreactive for γ-adaptin clathrin. immunofluorescent labeling isolated glands, immunoreactivity appeared to be concentrated in cells. primary cultures cells, immunocytochemical...
To investigate the involvement of carrier-mediated transport mechanisms in brimonidine retinal pigment epithelium (RPE).The [3H]-brimonidine bovine RPE-choroid explants was evaluated a modified Ussing chamber. The uptake [3H]brimonidine differentiated ARPE-19 cells cultured on permeable transwell filters.The into (choroid-to-retina [inward]) and out (retina-to-choroid [outward]) eye temperature dependent. Both inward outward decreased at 5 microM compared with 10 nM. melanin pigmentation RPE...