A5040422391- Prenatal Substance Exposure Effects
- Birth, Development, and Health
- Adenosine and Purinergic Signaling
- Cellular transport and secretion
- Invertebrate Immune Response Mechanisms
- Fatty Acid Research and Health
- Cancer, Lipids, and Metabolism
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Ion channel regulation and function
- Endoplasmic Reticulum Stress and Disease
- Aquaculture disease management and microbiota
- Neuroscience and Neuropharmacology Research
- Neuroendocrine Tumor Research Advances
- Folate and B Vitamins Research
- Genetic and Kidney Cyst Diseases
- Physiological and biochemical adaptations
- Protease and Inhibitor Mechanisms
- Teratomas and Epidermoid Cysts
- Phytochemical compounds biological activities
- Inflammatory mediators and NSAID effects
- Autophagy in Disease and Therapy
- Lung Cancer Research Studies
- Marine Sponges and Natural Products
- Head and Neck Anomalies
Universidad Católica del Norte
2014-2024
Cornell University
2013-2015
Pontificia Universidad Católica de Chile
2004-2011
Fundación Ciencia and Vida
2009
San Sebastián University
2009
Austral University of Chile
1997
Immune responses, as well reproduction, are energy-hungry processes, particularly in broadcast spawners such scallops. Thus, we aimed to explore the potential reproduction-immunity trade-off Argopecten purpuratus, a species with great economic importance for Chile and Peru. Hemocytes, key immunological cells mollusks, were center of this study, where addressed first time relation between reproductive stage, hemocyte metabolic energetics their capacity support immune responses at cellular...
Cholesterol-based membrane microdomains, or lipid rafts, are believed to play important, yet poorly defined, roles in protein trafficking and signal transduction. In polarized epithelial cells, the current view is that rafts involved apical but not basolateral transport from trans-Golgi network (TGN). We report here cholesterol required a post-TGN mechanism of regionalization. Permanently transfected Madin-Darby canine kidney cells segregated caveolae/raft-associated high-density lipoprotein...
Gastric Cancer (GC) is the one of most prevalent and leading causes cancer-induced deaths. Previously, we have found that purinergic P2Y2 receptor (P2Y2R) expression increased in GC samples as compared to adjacent healthy mucosa taken from GC-diagnosed patients. In this work, studied detail signaling gastric adenocarcinoma-derived cell lines, AGS, MKN-45 MKN-74 non-tumoral epithelial line, GES-1. GC-derived cells, detected several receptors, important differences GES-1 cells. Functional...
Sorting of glycosylphosphatidyl-inositol–anchored proteins (GPI-APs) in polarized epithelial cells is not fully understood. Oligomerization the Golgi complex has emerged as crucial event driving apical segregation GPI-APs two different kind cells, Madin–Darby canine kidney (MDCK) and Fisher rat thyroid (FRT) but whether mechanism conserved unknown. In MDCK cholesterol promotes GPI-AP oligomerization, well sorting GPI-APs. Here we show that FRT lack this cholesterol-driven oligomerization...
Gastric cancer (GC) is a major health concern worldwide, presenting complex pathophysiology that has hindered many therapeutic efforts so far. In this context, purinergic signaling emerges as promising pathway for intervention due to its known role in cell proliferation and migration. work, we explored more detail the of GC with several experimental approaches. First, measured extracellular ATP concentrations on GC-derived lines (AGS, MKN-45, MKN-74), finding higher levels than those...
Ethanol exposure increases oxidative stress in developing organs, including the brain. Antioxidant treatment during maternal ethanol ingestion improves behavioral deficits rodent models of fetal alcohol spectrum disorder (FASD). However, impact general antioxidant their adult offspring and Specific Reactive Species (ROS)-dependent mechanism, are not fully understood. We hypothesized that pre early postnatal (PEE) modifies redox homeostasis, particular NOX2 function reward signaling...
Gastric diseases represent a significant global public health challenge, characterized by molecular dysregulation in redox homeostasis and heightened oxidative stress. Although prior preclinical studies have demonstrated the cytoprotective antioxidant effects of alginate oligosaccharides (AOSs) through Nrf2 pathway, whether such mechanisms apply to gastric remains unclear. In this study, we used GES-1 cell line exposed hydrogen peroxide (H2O2) as damage model investigate impact AOS on...
In spite of the many key cellular functions chloride channels, mechanisms that mediate their subcellular localization are largely unknown. ClC-2 is a ubiquitous channel usually localized to basolateral domain epithelia regulates cell volume, ion transport, and acid-base balance; mice knocked out for blind sterile. Previous work suggested CLC-2 sorted basolaterally by TIFS(812)LL, dileucine motif in CLC-2's C-terminal domain. However, our silico modeling this was buried within channel's...
Prenatal ethanol exposure (PAE) induces behavioral maladptations in offspring, including a deficit memory formation which is part of the umbrella sign Fetal Alcohol spectrum disorder. Clinical and preclinical studies have shown that iron depletion exacerbates cognitive problems offspring exposed to utero PAE promotes dysregulation brain homeostasis. However, mechanisms underlying neuronal activity defects adolescent are unclear poorly understand. Here, we used rat model analyze mRNA protein...
Aims: Pre- and/or early postnatal ethanol exposure (prenatal alcohol [PAE]) impairs synaptic plasticity as well memory formation, but the mechanisms underlying these effects remain unclear. Both long-term potentiation (LTP) and spatial formation in hippocampus involve nicotinamide adenine dinucleotide phosphate oxidase type 2 (NOX2) enzyme. Previous studies have reported that N-methyl-d-aspartate receptor (NMDAR) activation increases NOX2-mediated superoxide generation, resulting inhibition...
The scallop Argopecten purpuratus is an important resource for Chilean and Peruvian aquaculture. Seed availability from commercial hatcheries critical due to recurrent massive mortalities associated with bacterial infections, especially during the veliger larval stage. immune response plays a crucial role in counteracting effects of such but being energetically costly, it potentially competes physiological morphological changes that occur early development, which are equally expensive....
Repeated exposure to alcohol increases retrieval of fear-conditioned memories, which facilitates, among other factors, the emergence post-traumatic stress disorder (PTSD). Individuals with PTSD are more likely develop and substance abuse related disorders. We assessed if prenatal early postnatal (PAE) increased susceptibility retain aversive memories this was associated subsequent heightened consumption. Pregnant Sprague-Dawley rats were exposed for 22 hr/day, throughout pregnancy until Day...
Knockout of the chloride channel protein 2 (CLC-2; CLCN2) results in fast progressing blindness mice. Retinal Pigment Epithelium (RPE) and photoreceptors undergo, parallel, rapid, profound morphological changes degeneration. Immunohistochemistry electron microscopy outer retina electroretinography CLC-2 KO mouse demonstrated normal morphology at postnatal day 2, followed by drastic RPE photoreceptor loss vision during first month. To investigate whether or are primary cause degeneration, we...
The objective of the present study was to investigate antioxidant and antiproliferative effects lemon extracts (LE) against human gastric cancer cells (SNU-1) normal epithelial (GES-1), their mechanism inducing cellular death. LE obtained by three extraction methods: conventional (CE), ultrasound (UAE) high hydrostatic pressure (HHPE). Experimental results show that method used obtain had a significant influence on capacity, proliferation, induction apoptosis in SNU-1 cells. showed be...
The aim of this study was to evaluate, for the first time, antiproliferative, apoptotic and diminishing effects anchored growth-independent capacity an ethanol macerate extract from Annona cherimola seed (EMCHS) in human gastric cancer cell line SNU-1. cells treated with EMCHS (20 μg/mL) significantly reduced form clones tumor cell. Moreover, 50 μg/mL induced apoptosis, as shown by Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone Annonacin) EMCHS, which could be...