A5070486404- Nanoparticles: synthesis and applications
- Advanced Nanomaterials in Catalysis
- Healthcare and Environmental Waste Management
- Electrochemical Analysis and Applications
- bioluminescence and chemiluminescence research
- Graphene and Nanomaterials Applications
- Bacterial biofilms and quorum sensing
- Polyoxometalates: Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- RNA modifications and cancer
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- Microplastics and Plastic Pollution
- Immune cells in cancer
- Microbial Metabolites in Food Biotechnology
- Chemistry and Chemical Engineering
- Gold and Silver Nanoparticles Synthesis and Applications
- Computational Drug Discovery Methods
- Medical and Biological Ozone Research
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Coal and Its By-products
- Biosimilars and Bioanalytical Methods
- Animal testing and alternatives
North Estonia Medical Centre
2022-2023
National Institute of Chemical Physics and Biophysics
2013-2022
University of Helsinki
2020-2021
Karolinska Institutet
2014-2018
Institute of Macromolecular Chemistry
2018
University of Tartu
2016
Tallinn University of Technology
2011-2012
Chemical Pharmaceutical Research Institute
1969
Background It is generally accepted that antibacterial properties of Ag nanoparticles (AgNPs) are dictated by their dissolved fraction. However, dissolution-based concept alone does not fully explain the toxic potency nanoparticulate silver compared to ions. Methodology/Principal Findings Herein, we demonstrated direct contact between bacterial cell and AgNPs' surface enhanced toxicity nanosilver. More specifically, cell-NP increased cellular uptake particle-associated ions – single ultimate...
Bacterial infections are one of the leading causes death worldwide. In case topical bacterial such as wound infections, silver (Ag) has historically been most widely used antibacterials. However, scientific publications have demonstrated adverse effects on human cells, ecotoxicity and insufficient antibacterial effect for complete elimination infections. The use Ag in form nanoparticles (NPs, 1-100 nm) allows to control release ions but is still not sufficient eliminate infection avoid...
Nanoparticles (NPs) have been shown to accumulate in organs, cross the blood–brain barrier and placenta, potential elicit developmental neurotoxicity (DNT). Here, we developed a human embryonic stem cell (hESC)-derived 3-dimensional (3-D) vitro model that allows for testing of neurotoxicants. Early central nervous system PAX6+ precursor cells were generated from hESCs differentiated further within 3-D structures. The was characterized neural marker expression revealing robust differentiation...
Silver nanoparticles (AgNP) are widely used in consumer products and medicine, mostly due to their excellent antimicrobial properties. One of the generally accepted antibacterial mechanisms AgNP is efficient contact with cells dissolution close vicinity bacterial cell envelope. Yet, primary mechanism wall damage events essential for bactericidal action not elucidated.In this study we a combination various assays differentiate adverse effects on envelope: outer membrane (OM) plasma (PM).We...
Within EU FP7 project NANOVALID, the (eco)toxicity of 7 well-characterized engineered nanomaterials (NMs) was evaluated by 15 bioassays in 4 laboratories. The highest tested nominal concentration NMs 100 mg/l. panel yielded following toxicity order: Ag > ZnO CuO TiO2 MWCNTs SiO2 Au. Ag, and proved very toxic majority assays, assumingly due to dissolution. latter supported parallel analysis respective soluble metal salts. most sensitive tests/species were Daphnia magna (towards NMs, 24-h EC50...
Abstract Carbon-based nanomaterials including carbon nanotubes (CNTs) have been shown to trigger inflammation. However, how these materials are ‘sensed’ by immune cells is not known. Here we compared the effects of two carbon-based nanomaterials, single-walled CNTs (SWCNTs) and graphene oxide (GO), on primary human monocyte-derived macrophages. Genome-wide transcriptomics assessment was performed at sub-cytotoxic doses. Pathway analysis microarray data revealed pronounced chemokine-encoding...
We show that in vitro toxicity assay based on inhibition of the bioluminescence recombinant Escherichia coli encoding thermostable luciferase from Photorhabdus luminescens is a versatile alternative to Vibrio fischeri MicrotoxTM test. Performance two luxCDABE-transformed E. MC1061 constructs (pDNlux) and (pSLlux) otherwise identical, but having 100-fold different background luminescence was compared with performance V. fischeri. The microplate luminometer kinetic Flash-Assay test format used...
The fat mass and obesity-associated protein (FTO), an RNA N6-methyladenosine (m6A) demethylase, is important regulator of central nervous system development, neuronal signaling disease. We present here the target-tailored development biological characterization small-molecule inhibitors FTO. active compounds were identified using high-throughput molecular docking dynamics screening ZINC compound library. In FTO binding activity-inhibition assays two best demonstrated Kd = 185 nM; IC50 1.46...
A set of nine recombinant heavy metal-specific luminescent bacterial sensors belonging to Gram-negative (Escherichia and Pseudomonas) Gram-positive (Staphylococcus Bacillus) genera containing various types metalresponse genetic elements was characterized for metal bioavailability studies. All strains were induced by Hg Cd five also Zn. As a lowest limit, the detecting 0.03 μg·L-1 Hg, 2 400 Limit determination depended mostly on metal-response element, whereas toxicity those metals towards...
The activity of a new class antimicrobials-polyoxometalate ionic liquids (POM-ILs)-is systematically investigated. prototype POM-ILs feature Keggin-type anions (α-SiW11 O398- ) and tetraalkylammonium ions as active cationic species. Antimicrobial tests the against important human pathogens show that variation alkyl chain length cation leads to significant changes in antimicrobial medically relevant Gram-negative bacteria Escherichia coli Pseudomonas aeruginosa, especially Gram-positive...
The analysis of (bio)available copper in complex environmental settings, including biological test media, is a challenging task. In this study, we demonstrated the potential recombinant Pseudomonas fluorescens-based biosensor for bioavailability CuSO4 and CuO nanoparticles (nano-CuO) seventeen different ecotoxicological microbiologial media. parallel, free Cu these media was analysed using Cu-ion selective electrode (Cu-ISE). case CuSO4, both bioavailable decreased greatly with increasing...