A5081562578- Lipid Membrane Structure and Behavior
- Molecular Junctions and Nanostructures
- Supramolecular Self-Assembly in Materials
- Force Microscopy Techniques and Applications
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Photoreceptor and optogenetics research
- Nanopore and Nanochannel Transport Studies
- Antimicrobial Peptides and Activities
- Photochromic and Fluorescence Chemistry
- Spectroscopy and Quantum Chemical Studies
- Cellular transport and secretion
- Neonatal and Maternal Infections
- Acoustic Wave Resonator Technologies
- Nanoplatforms for cancer theranostics
- Ion channel regulation and function
- Extracellular vesicles in disease
- Neuroscience and Neuropharmacology Research
- Protein Interaction Studies and Fluorescence Analysis
- Electrochemical sensors and biosensors
- Conducting polymers and applications
- RNA Interference and Gene Delivery
University of Warsaw
2019-2025
Daptomycin is known as an effective antibiotic lipopeptide which shows activity against the number of Gram-positive pathogens. Its primary target bacterial cell membrane. However, detailed mechanism daptomycin action still subject to debate. In this paper, we have investigated interactions between and model lipid films composed negatively charged phosphatidylglycerols cardiolipin. order evaluate effect on molecular organization properties assemblies, used surface pressure measurements...
Cerivastatin belongs to the class of statins, drugs lowering blood cholesterol level by inhibiting enzyme HMG-CoA reductase. The is located in endoplasmic reticulum (ER) membrane, which enriched and sphingolipids. Therefore, present study an equimolar mixture 1,2-dioleoylo-sn‑glycero-3-phosphocholine (DOPC), (Chol) egg sphingomyelin (SM) was used prepare a simple model ER membrane using Langmuir technique. DOPC:Chol:SM 1:1:1 monolayers were characterized surface pressure measurements...
A model biomimetic system for the study of protein reconstitution or drug interactions should include lipid rafts in mixed monolayer, since they are usually domains embedding membrane proteins and peptides. Four films composed three components: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), cholesterol (Chol) sphingomyelin (SM) different molar ratios were proposed investigated using surface pressure measurements thermodynamic analysis monolayers at air-water interface imaged by Brewster...
The interactions of anthracyclines with biological membranes strongly depend on the drug lipophilicity, which might also determine specific affinity to cholesterol molecules. Therefore, in this work we show studies concerning effect two selected anthracyclines, daunorubicin (DNR) and idarubicin (IDA) simple models healthy (DMPC:Chol 7:3) cancer cells increased level 3:7) as well pure monolayers prepared at air-water interface supported gold surface. It has been shown that more lipophilic IDA...
Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods for preparation planar films, self-assembly bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism formation and electrochemical properties films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption occurs by replacement interfacial water it leads a double bilayer...
Abstract Sparsely tethered bilayer lipid membrane (stBLM) is a popular model considered to mimic biological membranes. The advantage of this system the molecular architecture that provides water environment on both sides membrane. In work, we have coupled electrochemical measurements with surface enhanced infrared absorption spectroscopy and atomic force microscopy evaluate effect electric field physicochemical properties stBLM composed 1,2‐dimyristoyl‐ sn ‐glycero‐3‐phosphocholine...
Abstract This concept paper elucidates the possible influence of electrical double layer (EDL) and polyelectrolytic nature bacterial cell walls on behavior antimicrobial peptides (AMPs) lipopeptides. It proposes that presence EDL adjacent to lipid membranes may play a crucial role in inducing peptide aggregation conformational changes, thus affecting their activity. Furthermore, it emphasizes negatively charged components both Gram‐positive Gram‐negative act as polyelectrolytes, effectively...
Personalized medicine is a new approach to modern oncology. Here, facilitate the application of extracellular vesicles (EVs) derived from lung cancer cells as potent advanced therapy medicinal products in cancer, EV membrane was functionalized with specific ligand for targeting purposes. In this role, most effective heptapeptide binding (PTHTRWA) used. The functionalization process surface performed through C- or N-terminal end heptapeptide. To prove activity EVs PTHTRWA, both model lipid...
We have designed and synthesized two α-helicomimetic foldamers composed of six urea residues with terminal ferrocene cysteamine moieties. The direction the net dipole moment in these compounds was opposite, it directed either toward moiety or cysteine. found that affects electrochemical behavior oligourea monolayers deposited on a gold electrode. By employing situ surface-enhanced infrared reflection-absorption spectroscopy, we were able to demonstrate electric field-induced dynamics...
The lipid cubic phase (LCP) is a nanomaterial composed of water channels surrounded by bilayers. LCPs are stable at room temperature and biocompatible. These features make the phases similar to biological membranes, hence, favorable for embedding membrane proteins. We show that monoolein deposited on electrode forms 3D bilayer film convenient electrochemical investigations In this research, we studied effect an ionophoric peptide, gramicidin A (gA), structure properties LCP film. identity...
Many biochemical processes related to proper homeostasis take place in cell membranes. The key molecules involved these are proteins, including transmembrane proteins. These macromolecules still challenge the understanding of their function within membrane. Biomimetic models that mimic properties membrane can help understand functionality. Unfortunately, preserving native protein structure such systems is problematic. A possible solution this problem involves use bicelles. Their unique make...
We have designed and synthesized a helical cysteamine-terminated oligourea foldamer composed of ten urea residues featuring side carboxyl amine groups. The group is located in proximity to the C-terminus hence at negative pole helix dipole. close N-terminus positive Beyond already remarkable dipole moment inherent 2.5 helices, incorporation additional charges originating from carboxylic groups supposed impact overall charge distribution along molecule. These molecules were self-assembled...
Many biochemical processes related to proper homeostasis take place in cell membranes. The key molecules involved these are proteins, including transmembrane proteins. These macromolecules still challenge the understanding of their function within membrane. Biomimetic models that mimic properties membrane can help understand and functions. Unfortunately, preserving native protein structure such systems is quite problematic. A possible solution this problem involves use bicelles. Their unique...