Herein, an electrochemical (EC)–photoelectrochemical (PEC) dual-mode biosensor was constructed for cytokeratin 19 fragment 21-1 (CYFRA21-1) assay based on the dual-signaling ratiometric strategy and "on–off–on" PEC method. The indium tin oxide (ITO) electrode modified by 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA)@C60 gold nanoparticles (Au NPs), double-stranded DNA composed of thiol/methylene blue (MB)-labeled single-stranded (ssDNA) (S0-MB) antibody/ferrocene (Fc)-labeled ssDNA...

Highly sensitive and selective microRNA (miRNA) assay is of great significance for disease diagnosis therapy. Herein, a magnetic-assisted electrochemistry (EC)–photoelectrochemistry (PEC) dual-mode biosensing platform was developed miRNA-210 detection based on dual-signaling EC photocurrent-polarity-switching PEC strategies. Porous magnetic Fe3O4 octahedra with large surface area were synthesized by calcining Fe-based metal–organic frameworks. Subsequently, the photoelectric materials...

Mercury ion (Hg2+) is one of the most harmful heavy metal ions with greatest impact on public health. Herein, based excellent catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) and strong photocurrent-polarity-switching ability to SnS2 photoanode split G-quadruplex–hemin complex, magnetic NiCo2O4@SiO2–NH2 sphere-assisted colorimetric photoelectrochemical (PEC) dual-mode sensing platform was developed for Hg2+ assay. First, amino-labelled single-stranded DNA1 (S1) immobilized then...

A compact and clamped CHA-control-HCR (CCH) circuitry system, specifically for amplified multi-microRNA imaging, is developed to precisely distinguish cancer cells from normal cells.

Abstract Synthetic biochemical circuits (e.g., DNA circuits) remain at the forefront of intracellular biosensing tasks yet are hindered by undesired off‐site activation and accompanying signal leakage. Herein, study attempts to overcome this limitation developing a simple‐yet‐powerful endogenous glutathione (GSH)‐regulating tactic that permits robust distinguishable on‐site microRNA (miRNA) imaging under disturbed redox homeostasis. Specifically, hierarchically activated catalytic (HAD)...

Epigenetic modification plays an indispensable role in regulating routine molecular signaling pathways, yet it is rarely used to modulate self-assembly networks. Herein, we constructed a bioorthogonal demethylase-stimulated DNA circuitry (DSC) system for high-fidelity imaging of microRNA (miRNA) live cells and mice by eliminating undesired off-site signal leakage. The simple robust DSC composed primary cell-specific regulation (CR) module ultimate signal-transducing amplifier (SA) module....

Bamboo-like N-doped carbon nanotubes wrapping Co nanoparticles (Co@BNCNTs) derived from a single precursor Co-MOF exhibited remarkable ORR electrocatalytic performance with long-term durability, due to the synergetic interaction between NCNTs and nanoparticles.

A reduction-cleavable polyphosphoester-camptothecin (CPT) prodrug tailored for enhancing drug loading content and triggering release has been prepared applied in tumor chemotherapy.

Water pollution presents a significant environmental concern on earth. Herein, due to the serious harmfulness of arsenate [As(V)], an iron phthalocyanine (FePc)-induced switchable photocurrent-polarity platform was developed for highly selective assay As(V). First, magnetic Co3O4–Fe3O4 cubes were obtained by calcination CoFe Prussian blue analogue and then functionalized with oligonucleotide (S1). In presence As(V), S1 could be released based stronger affinity between As(V) cubes. After...

Abstract The sensing performance of DNAzymes in live cells is tremendously hampered by the inefficient and inhomogeneous delivery DNAzyme probes their incontrollable off‐site activation, originating from susceptibility to nuclease digestion. This requires development a more compact robust DNAzyme‐delivering system with site‐specific activation property. Herein, highly Zn@DDz nanoplatform constructed integrating unimolecular microRNA‐responsive DNA‐cleaving (DDz) probe requisite Zn 2+ ‐ion...

Building dynamic biological networks, especially DNA circuits, has provided a powerful prospect for exploring the intrinsic regulation processes of live cells. Nevertheless, efficient intracellular microRNA analysis, available multi-component circuits are constrained by their limited operating speed and efficiency due to free diffusion reactants. Herein, we developed an accelerated Y-shaped catalytic (YDC) circuit high-efficiency imaging microRNA. By grafting hairpin assembly (CHA) reactants...

Colorectal cancer (CRC) is a major cause of cancer-related deaths in humans, and effective treatments are still needed clinical practice. Despite significant developments anticancer drugs inhibitors, their poor stability, water solubility, cellular membrane permeability limit therapeutic efficacy. To address these issues, multifunctional CaCO3 nanoparticles loaded with Curcumin (Cur) protein deacetylase (HDAC) inhibitor QTX125, coated hyaluronic acid (HA) (CaCO3@Cur@QTX125@HA), were prepared...

Circular RNAs (circRNAs) have emerged as regulators of cancer progression, including non-small cell lung (NSCLC). Tanreqing (TRQ), a traditional Chinese medicine, is used clinically for respiratory diseases. RT-qPCR quantified circ-WDR78 expression in NSCLC cells. Cell growth, apoptosis, invasion, and migration were assessed by functional assays. RNA-binding protein immunoprecipitation (RIP), luciferase reporter, RNA pull-down assays determined the competing endogenous (ceRNA) network...

The detection of lysine acetyltransferases is crucial for diagnosing and treating lung cancer, highlighting the necessity highly efficient methods. We developed a portable, accurate, sensitive technique using fast-scan cyclic voltammetry (FSCV) to determine activity acetyltransferase TIP60, employing novel miniature electrochemical sensor. This approach involves compact cell, merely 3 mm in diameter, that holds solutions up 50 μL, equipped with conductive indium tin oxide working electrode....