Abstract Pyroptosis, a form of programmed cell death (PCD), has garnered increasing attention as it relates to innate immunity and diseases. However, the involvement pyroptosis in mechanism by which lobaplatin acts against colorectal cancer (CRC) is unclear. Our study revealed that treatment with reduced viability HT-29 HCT116 cells dose-dependent manner. Morphologically, treated exhibited microscopic features swelling large bubbles emerging from plasma membrane, transmission electron...

Repeated and long-term oxaliplatin therapy leads to drug resistance severe adverse events, which limit its clinical use. These difficulties highlight the importance of identifying potent specific combinations enhance antitumor effects oxaliplatin. The farnesoid X receptor (FXR) deficiency in colorectal cancer (CRC) suggests that restoring FXR function might be a promising strategy for CRC treatment. A combination study showed GW4064 acted synergistically with colon cells. plus inhibited cell...

Background: Presently, colistin is commercially available in two different forms, namely, sulfate and its sulphomethylated derivative, colistimethate sodium (CMS). However, the currently reported studies, most of clinical studies on for parenteral use are referred to as CMS. Data pharmacokinetics (PK), efficacy, side effects have not been reported. Methods: This retrospective study was performed carbapenem-resistant organism (CRO)-infected patients treated with more than 72 h. The population...

The gasdermins (GSDMs) family is proposed to be pore-forming effector proteins that cause cell membrane permeabilization and pyroptosis. Despite our increasing knowledge of GSDMD, GSDME GSDMB, the biological functions regulation GSDM expression activation remain elusive for most GSDMs. In this study, we analyzed molecular characteristics oncogenic role genes systematically.TCGA, CCLE, cBioPortal, GEPIA, CellMiner BioGRID databases were utilized in study. Immunohistochemical analysis a series...

Duodenogastric reflux (DGR) is closely associated with gastric inflammation and tumorigenesis; however, the precise mechanism unclear. Hence, we aim to clarify this molecular design an effective therapeutic strategy based on it. The present study found that DGR induced TXNIP/NLRP3 inflammasome activation triggered pyroptosis in mucosa

BACKGROUND:Intra-abdominal adhesion is one of the most common complications after abdominal surgery. The efficacy current treatments for intra-abdominal unsatisfactory. In this study, we investigated effect gallic acid on prevention and treatment adhesions surgery using an rat model. MATERIAL AND METHODS:The experimental rats were randomly divided into sham operation group, control chitosan 3 groups different concentrations. All except those in group received cecal abrasion to induce...

MicroRNA (miR)‑181a is a member of the miR‑181 family that serves key role in pathogenesis various cancer types. The present study aimed to investigate interaction between miR‑181a and Ras association domain protein1 isoform A (RASSF1A), their roles gastric carcinogenesis. RASSF1A was assessed cell lines tissues. direct binding identified using luciferase reporting gene system. effects on growth, cycle apoptosis were with Cell Counting Kit‑8 assay flow cytometry. division 25A (CDC25A),...

Gastric cancer is among the most common gastrointestinal malignancies. Recent studies have suggested that bone morphogenetic protein-2 (BMP2) related to development and progression of various cancers. Meanwhile, evidence suggests BMP2 might lead epigenetic changes in gastric cancer. Thus, we investigated whether plays a role via regulation. Cell viability, colony formation, cell cycle assays were performed assess effect recombinant human (rhBMP2) cells. LDN-193189 Noggins used as antagonists...

Colorectal cancer (CRC) remains a leading cause of cancer-related death worldwide. Cetuximab, in combination with chemotherapy, is effective for treating patients wild-type KRAS/BRAF metastatic CRC (mCRC). However, intrinsic or acquired drug resistance often limits the use cetuximab. In this study, we investigated potential co-treatment 3-Bromopyruvate (3-BP) and cetuximab to overcome CRC, both vitro vivo. Our results demonstrated that 3-BP synergistically induced an antiproliferative effect...

Loss of caudal type homeobox 2 (CDX2) is associated with the development human colorectal cancer, while telomerase reverse transcriptase (hTERT) frequently occurs in variety cancers. We investigated effects restoration CDX2 expression using a hypoxia-inducible hTERT promoter-driven vector (pLVX-5HRE-hTERTp-CDX2-3FLAG) on colon cancer cell viability, cycle distribution, apoptosis, colony formation, invasion ability and xenograft tumor growth nude mice. overexpression significantly inhibited...

Forkhead box (FOX) A1 is a member of the FOX family transcription factors, which serve function in numerous types tumor. The present study assessed potential role FOXA1 human epithelial ovarian carcinoma (EOC). Total RNA was isolated from 16 fresh-frozen EOC tumors with paired corresponding non-malignant epithelium tissues, and expression analyzed using reverse transcription-quantitative polymerase chain reaction. Immunohistochemical analysis performed to evaluate 110 tissue specimens...

Abstract Zinc-finger of the cerebellum 2 (Zic2) is widely implicated in cancers, but role Zic2 tumorigenesis bilateral. A recent study indicated that could render colon cancer cells more resistant to low glucose-induced apoptosis. However, functional roles and underlying molecular mechanism remain elusive. Herein, we demonstrated was highly expressed tissues correlated with poor survival. Knockdown inhibited cell growth, arrested cycle transition from G0/G1 S phase, suppressed tumor sphere...

Role of aquaporin-8 (AQP8) in cervical cancer has not been fully elucidated.We aim to explore the impacts AQP8 on viability, apoptosis and metastasis cells.AQP8 protein expression carcinoma specimens cell lines was detected by IHC western blot analysis. Lentivirus-mediated transfection used upregulate knockdown cells. Cell viability were assessed CCK-8 flow cytometry assays, respectively. Transwell experiments conducted investigate invasive migratory capabilities. EMT-related markers...

Caudal-related homeobox protein 2 (CDX2), a tumor suppressor in the adult colon, is overexpressed under non-cancer specific cytomegalovirus promoter certain cells; furthermore, non-specific expression of CDX2 may result aberrant side effects normal cells. The human telomerase reverse transcriptase (hTERT) active majority cancer cells but not Hypoxia key feature solid tumors, and targeted genes be significantly upregulated by five copies hypoxia-response elements (HREs) hypoxic conditions....

The human deafness, autosomal dominant 5 gene (DFNA5), a newly discovered executor of pyroptosis, has been strongly implicated in the tumorigenesis several cancers. However, an understanding functional role DFNA5 development and progression colorectal cancer (CRC) is limited. In this study, we demonstrated that was downregulated CRC tissues. Ectopic expression inhibited tumor cell growth vitro, retarded formation vivo, blocked cell-cycle transition from G0/G1 to S phase, whereas knockdown...

The Escherichia coli purine nucleoside phosphorylase/Fludarabine phosphate (ePNP/Fludara) suicide system has several drawbacks, such as side-effects and the low efficiency of ePNP expression. In this study, we evaluated antitumor effects dual-specific 8HSEs-hTERTp-ePNP/Fludara under hyperthermia in vitro vivo. Luciferase activities from 8HSEs‑hTERT CMV promoters were compared using dual luciferase assay SW480 (high hTERT expression) MKN74 cells (hTERT-negative) presence absence hyperthermia....

Abstract Background Repeated and long-term oxaliplatin therapy leads to drug resistance severe adverse events, which limit its clinical use. These difficulties highlight the importance of identifying potent specific combinations enhance antitumor effects oxaliplatin. The farnesoid X receptor (FXR) deficiency in colorectal cancer (CRC) suggests that restoring FXR function might be a promising strategy for CRC treatment. Methods A series vitro vivo experiments were conducted assess effect...