A5100754966- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Glutathione Transferases and Polymorphisms
- Cancer-related Molecular Pathways
- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- PI3K/AKT/mTOR signaling in cancer
- Immunotherapy and Immune Responses
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- PARP inhibition in cancer therapy
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- Antibiotics Pharmacokinetics and Efficacy
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
Wenzhou Medical University
2022-2023
First Affiliated Hospital of Wenzhou Medical University
2022-2023
Peking University
2018-2023
Yangtze University
2022
The University of Texas MD Anderson Cancer Center
2002-2021
Shantou University
2015-2020
Inner Mongolia Medical University
2018-2020
Cancer Genetics (United States)
2007
Epidermal growth factor receptor (EGFR) mutations typically occur in exons 18-21 and are established driver non-small cell lung cancer (NSCLC)1-3. Targeted therapies approved for patients with 'classical' a small number of other mutations4-6. However, effective have not been identified additional EGFR mutations. Furthermore, the frequency effects atypical on drug sensitivity unknown1,3,7-10. Here we characterize mutational landscape 16,715 EGFR-mutant NSCLC, establish structure-function...
// Hongyu Li 1, 4 , Jing Hu 1 Shuhong Wu Wang Xiaobo Cao Xiaoshan Zhang Bingbing Dai Mengru Ruping Shao Ran Mourad Majidi Lin Ji John V. Heymach 2 Michael 3 Shiyang Pan 5 Minna 6 Reza J. Mehran Stephen G. Swisher Jack A. Roth and Bingliang Fang Department of Thoracic Cardiovascular Surgery, University Texas MD Anderson Cancer Center, Houston, Texas, USA Thoracic/Head & Neck Medical Oncology, Lymphoma, Jilin Province Hospital, Changchun, Jilin, China Laboratory Medicine, The First...
Abstract Development of candidate cancer treatments is a resource-intensive process, with the research community continuing to investigate options beyond static genomic characterization. Toward this goal, we have established landscapes 536 patient-derived xenograft (PDX) models across 25 types, together mutation, copy number, fusion, transcriptomic profiles, and NCI-MATCH arms. Compared human tumors, PDXs typically higher purity fit dynamic driver events molecular properties via multiple...
Abstract Glutathione (GSH)/GSH reductase (GSR) and thioredoxin/thioredoxin (TXNRD) are two major compensating thiol-dependent antioxidant pathways that maintain protein dithiol/disulfide balance. We hypothesized functional deficiency in one of these systems would render cells dependent on compensation by the other system for survival, providing a mechanism-based synthetic lethality approach treatment cancers. The human GSR gene is located chromosome 8p12, region frequently lost deletion was...
The DREAM complex orchestrates cell quiescence and the cycle. However, how is deregulated in cancer remains elusive. Here, we report that PAF (PCLAF/KIAA0101) drives exit to promote lung tumorigenesis by remodeling complex. highly expressed adenocarcinoma (LUAD) associated with poor prognosis. Importantly, Paf knockout markedly suppressed LUAD development mouse models. depletion induced growth arrest. required for global expression of cell-cycle genes controlled repressive Mechanistically,...
Mutations in Artemis both humans and mice result severe combined immunodeficiency due to a defect V(D)J recombination. In addition, mutants are radiosensitive chromosomally unstable, which has been attributed nonhomologous end joining (NHEJ). We show here, however, that Artemis-depleted cell extracts not defective NHEJ Artemis-deficient cells have normal repair kinetics of double-strand breaks after exposure ionizing radiation (IR). is shown, interact with known cycle checkpoint proteins be...
The removal of interstrand cross-links (ICLs) from DNA in higher eucaryotes is not well understood. Here, we show that processing psoralen ICLs mammalian cell extracts dependent upon the mismatch repair complex hMutSβ but hMutSα or hMlh1. also nucleotide excision proteins Ercc1 and Xpf other components stage this pathway Fanconi anemia proteins. Products formed during vitro reaction indicated ICL has been removed uncoupled cross-linked substrate extracts. Finally, shown to specifically bind...
Background: Presently, colistin is commercially available in two different forms, namely, sulfate and its sulphomethylated derivative, colistimethate sodium (CMS). However, the currently reported studies, most of clinical studies on for parenteral use are referred to as CMS. Data pharmacokinetics (PK), efficacy, side effects have not been reported. Methods: This retrospective study was performed carbapenem-resistant organism (CRO)-infected patients treated with more than 72 h. The population...
Patient-derived xenograft (PDX) models increasingly are used in translational research. However, the engraftment rates of patient tumor samples immunodeficient mice to PDX vary greatly.Tumor tissue from 308 patients with non-small cell lung cancer were implanted mice. The followed for 1.5 approximately 6 years. authors performed histological analysis PDXs and some residual tissues failed growth at 1 year after implantation. Quantitative polymerase chain reaction enzyme-linked immunoadsorbent...
Artemis is a phospho-protein that has been shown to have roles in V(D)J recombination, nonhomologous end-joining of double-strand breaks, and regulation the DNA damage-induced G(2)/M cell cycle checkpoint. Here, we identified four sites are phosphorylated response ionizing radiation (IR) show ATM major kinase responsible for these modifications. Two sites, S534 S538, rapid phosphorylation dephosphorylation, other two S516 S645, exhibit prolonged phosphorylation. Mutation both latter residues...
Akt is a critical mediator of the oncogenic PI3K pathway, and its activation regulated by kinases phosphatases acting in opposition. We report here existence novel protein complex that composed minimally Akt, PHLPP1, PHLPP2, FANCI, FANCD2, USP1 UAF1. Our studies show depletion but not FANCD2 or USP1, results increased phosphorylation Akt. This due to reduction interaction between PHLPP1 absence FANCI. In response DNA damage growth factor treatment, interactions FANCI are reduced consistent...
The dysfunction and apoptosis of vascular endothelial cells are the initiating links in formation atherosclerosis. N6-methyladenosine (m6A) is an extremely extensive RNA methylation modification its abnormality leads to occurrence various human diseases. In this study, we explored effects demethylase α-ketoglutarate-dependent dioxygenase ALKB homolog 5 (ALKBH5) on TNF-α-induced umbilical vein (HUVECs). TNF-α-treated HUVECs, expression ALKBH5 was significantly decreased. overexpression...
Abstract Organoid technology offers sophisticated in vitro human models for basic research and drug development. However, low batch-to-batch reproducibility high cost due to laborious procedures materials prevent organoid culture standardization automation high-throughput applications. Here, using a novel platform based on the findings that Pluronic F-127 (PF-127) could trigger highly uniform spheroid assembly through mechanism different from plate coating, we develop one-pot differentiation...
An efficient aptasensor was developed in which graphene oxide (GO) employed as an indicator for both electrochemical impedance spectroscopy and electrochemiluminescence (ECL) signal generation. The fabricated by self-assembling the ECL probe of a thiolated adenosine triphosphate binding aptamer (ABA) tagged with Ru complex (Ru(bpy)3(2+) derivatives) onto surface gold nanoparticle (AuNP) modified glassy carbon electrode (GCE). ABA immobilized AuNP GCE could strongly adsorb GO due to strong...
Artemis, a member of the SNM1 gene family, is known phosphorylation target ATM, ATR, and DNA-PKcs. We have previously identified two serine residues in Artemis (Ser(516) Ser(645)) that are subject to by ATM involved mediating recovery from G(2)/M checkpoint response ionizing radiation. Here we show these same sites also phosphorylated ATR various types replication stress including UVC, aphidicolin, hydroxyurea. mutation Ser(516) Ser(645) causes prolonged S phase after treatment with UV or...
Artemis, a member of the SNM1 gene family, is multifunctional phospho-protein that has been shown to have important roles in V(D)J recombination, DNA double strand break repair, and stress-induced cell-cycle checkpoint regulation. We show here Artemis interacts with Cul4A-DDB1 E3 ubiquitin ligase via direct interaction substrate-specificity receptor DDB2. Furthermore, also CDK inhibitor tumor suppressor p27, substrate ligase, both DDB2 are required for degradation p27 mediated by this...
Spindle poisons represent an important class of anticancer drugs that act by interfering with microtubule polymerization and dynamics thereby induce mitotic checkpoints apoptosis. Here we show mammalian SNM1 functions in early stress checkpoint is distinct from the well-characterized spindle regulates metaphase-to-anaphase transition. Specifically, found compared to wild-type cells, Snm1-deficient mouse embryonic fibroblasts exposed exhibited elevated levels micronucleus formation, decreased...
The NONO gene is located on chromosome Xq13.1 and encodes a nuclear protein involved in RNA synthesis, transcriptional regulation, DNA repair. Hemizygous variants have been reported to cause mental retardation, X-linked, syndromic 34 (MRXS34) males. Due the scarcity of clinical reports, characteristics mutation spectrum NONO-related disorder not entirely determined.We fetus with hypoplastic left heart syndrome, performed comprehensive genotyping examination, including copy-number variation...
Anti-PD-1 and anti-PD-L1 immunotherapy has provided a new therapeutic opportunity for treatment of advanced-stage non-small cell lung cancer (NSCLC). However, overall objective response rates are approximately 15%–25% in all NSCLC patients who receive anti-PD therapy. Therefore, strategies to overcome primary resistance urgently needed. We hypothesized that the barrier success therapy most can be by stimulating lymphocyte infiltration at sites through locoregional virotherapy. To this end,...