A5100379193- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Pancreatic and Hepatic Oncology Research
- Legionella and Acanthamoeba research
- Chronic Lymphocytic Leukemia Research
- Renal cell carcinoma treatment
- Ferroptosis and cancer prognosis
- Genomics and Rare Diseases
- Viral Infections and Outbreaks Research
- Grief, Bereavement, and Mental Health
- Tuberous Sclerosis Complex Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
Harvard University
2005-2025
Massachusetts General Hospital
2005-2025
University of Kentucky
2019-2024
Leidos (United States)
2019-2024
Frederick National Laboratory for Cancer Research
2018-2024
Markey Cancer Center
2019-2024
Sichuan University
2022-2024
University of the Basque Country
2024
West China Second University Hospital of Sichuan University
2022-2024
Dana-Farber Cancer Institute
2010-2024
Background Tumor mutational burden (TMB), defined as the number of somatic mutations per megabase interrogated genomic sequence, demonstrates predictive biomarker potential for identification patients with cancer most likely to respond immune checkpoint inhibitors. TMB is optimally calculated by whole exome sequencing (WES), but next-generation targeted panels provide estimates in a time-effective and cost-effective manner. However, differences panel size gene coverage, addition underlying...
Abstract Patient-derived xenografts (PDXs) are resected human tumors engrafted into mice for preclinical studies and therapeutic testing. It has been proposed that the mouse host affects tumor evolution during PDX engraftment propagation, affecting accuracy of modeling cancer. Here, we exhaustively analyze copy number alterations (CNAs) in 1,451 matched patient (PT) samples from 509 models. CNA inferences based on DNA sequencing microarray data displayed substantially higher resolution...
The CIITAdel keeps viruses at bay A better understanding of cellular mechanisms involved in viral resistance is needed for the next generation antiviral therapies. Bruchez et al. used a transposon-mediated gene-activation screen to search previously unreported host restriction factors Ebola virus (see Perspective by Wells and Coyne). authors found that transcription factor, major histocompatibility complex class II transactivator (CIITA), induces human cell lines directing expression p41...
IntroductionVariable genomic breakpoints have been identified through the application of target-capture DNA next-generation sequencing (NGS) for ALK, ROS1, and RET fusion detection in NSCLC. We investigated whether breakpoint location can predict matched targeted therapy efficacy.MethodsNSCLCs were analyzed by NGS, target-specific RNA whole-transcriptome sequencing, immunohistochemistry.ResultsIn total, 3787 NSCLC samples analyzed. NGS detected fusions 241, 59, 76 cases, respectively. These...
Abstract Development of candidate cancer treatments is a resource-intensive process, with the research community continuing to investigate options beyond static genomic characterization. Toward this goal, we have established landscapes 536 patient-derived xenograft (PDX) models across 25 types, together mutation, copy number, fusion, transcriptomic profiles, and NCI-MATCH arms. Compared human tumors, PDXs typically higher purity fit dynamic driver events molecular properties via multiple...
Amino acids are transported across the plasma membrane of plant cells by proton-amino acid symports. We report here successful cloning a neutral amino carrier functional complementation. A histidine transport deletion mutant Saccharomyces cerevisiae was transformed with an Arabidopsis thaliana cDNA library constructed in yeast expression vector. Forty transformants, out 10(5), allowed growth on histidine-limiting medium. The acquired ability to grow low shown be strictly dependent protein...
Translation initiation factor eukaryotic translation 4E (eIF4E) plays a key role in regulation of cellular proliferation.Its effects on the m 7 GpppN mRNA cap are critical because overexpression eIF4E transforms cells, and function is rate-limiting for G 1 passage.Although we identified as c-Myc target, little else known about its transcriptional regulation.Previously, described an element at position ؊25 (TTACCCCCCCTT) that was promoter function.Here report this sequence (named 4EBE, basal...
Mannose-binding lectin (MBL) is a key soluble effector of the innate immune system that recognizes pathogen-specific surface glycans. Surprisingly, low-producing MBL genetic variants may predispose children and immunocompromised individuals to infectious diseases are more common than would be expected in human populations. Since certain defense molecules, such as immunoglobulins, can exploited by invasive pathogens, we hypothesized might also enhance infections some circumstances....
Hepatocyte nuclear factor-1 alpha (HNF-1 alpha) and HNF-1 beta are transcription factors that contain a divergent homeodomain, bind to DNA as homo- or heterodimers, act regulate of many genes expressed primarily in liver. We show regulatory for sucrase-isomaltase (SI), the gene which is exclusively absorptive enterocytes small intestine. two evolutionarily conserved sites SI promoter, previously named SIF2 SIF3, with different relative affinities. binds SIF3 element greater affinity than...
Purpose Nucleolar spindle-associated protein (NuSAP1) is an important mitosis-related protein, and aberrant NuSAP1 expression associated with abnormal spindles mitosis. This study investigated the prognostic value of in breast cancer. Methods Two sets tissue microarrays (TMAs) that included samples from 450 cancer patients were constructed, which 250 training set other 200 validation set. Immunohistochemical staining was performed to determine levels. A Kaplan-Meier analysis used estimate...
Background: Clinical trials frequently reported anaplastic lymphoma kinase tyrosine inhibitors (ALK-TKIs) associated with cardiac adverse drug events (AEs) but minimal postmarketing data. We aimed to research real-world disorders ALK-TKIs based on the Food and Drug Administration Adverse Event Reporting System (FAERS). Methods: Extract reports from FAERS first quarter of 2016 second 2021 were obtained. Data mining was carried out using disproportionality analysis determine clinical...
The progression from preinvasive lesion to invasive carcinoma is a critical step contributing breast cancer lethality. We identified downregulation of milk fat globule-EGF factor 8 (MFG-E8) as contributor using microarray analysis laser capture microdissected (LCM) tissues. first MFG-E8 in lesions transgenic mammary tumor models, which were confirmed LCM-isolated human ductal carcinomas compared with patient-matched normal In situ analyses expression estrogen receptor (ER) positive cases its...
Abstract The c-myc oncogene plays a key role in cellular growth control, and translation initiation factors are among the transcriptional targets of Myc. Here, we describe defect control myc-null cells due to alterations mammalian target rapamycin (mTOR) pathway. Myc loss increased sensitivity dominant inhibition eukaryotic factor 4E function. Polysomal profiles myc−/− revealed decreased rates, which were accompanied by 40S/60S ribosomal subunit ratios. Because 40S small contains regulatory...
Yin Yang 1 (YY1) is a critical transcription factor controlling cell proliferation, development and DNA damage responses. Retrotranspositions have independently generated additional YY family members in multiple species. Although Drosophila YY1 [pleiohomeotic (Pho)] its homolog [pleiohomeotic-like (Phol)] redundantly control homeotic gene expression, the regulatory contributions of YY1-homologs not yet been examined other Indeed, targets for mammalian YY2 are completely unknown. Using set...
Retroviral vectors including lentiviral are commonly used tools to stably express transgenes or RNA molecules in mammalian cells. Their utilities roughly divided into two categories, stable overexpression of and molecules, which requires maximal transduction efficiency, functional selection with retrovirus (RV)-based libraries, takes advantage retroviral superinfection resistance. However, the dynamic features RV-mediated not well characterized. Here, we engineered murine stem cell...
Background Despite the fact that numerous clinical and preclinical studies have demonstrated synergistic effects of combining antiangiogenic or chemotherapy with immunotherapy, no data been found to indicate combination therapy is more effective safer as second-line therapy. Methods We retrospectively compared effectiveness safety ICIs plus rh-endostatin in patients advanced non-small cell lung cancer (NSCLC). The evaluation indicators this study were progression-free survival (PFS),...
This study investigated the predictive value of β2-microglobulin (β2-MG) on prognosis diffuse large B-cell lymphoma (DLBCL) patients. Patients with DLBCL diagnosed by pathologic biopsy were collected from January 2021 through December 2022, and a total 98 patients finally included. The grouped according to their prognosis, i.e., into survival group (n = 69) death 29). critical β2-MG for in was obtained ROC curve. Kaplan Meier curve plotted analyze relationship between overall patients,...
Targeted next-generation sequencing is a powerful method to comprehensively identify biomarkers for cancer. Starting material currently either DNA or RNA different variations, but splitting 2 assays burdensome and sometimes unpractical, causing delay complete lack of detection critical events, in particular, potent targetable fusion events. An assay that analyzes both templates streamlined process eagerly needed.We developed single-tube, dual-template an integrated bioinformatics pipeline...