A5027098755- HIV Research and Treatment
- Reproductive tract infections research
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Complement system in diseases
- Reproductive System and Pregnancy
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Syphilis Diagnosis and Treatment
- Adolescent Sexual and Reproductive Health
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Cervical Cancer and HPV Research
- Blood groups and transfusion
- Gut microbiota and health
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Immune Response and Inflammation
- HIV-related health complications and treatments
- SARS-CoV-2 and COVID-19 Research
- Pelvic floor disorders treatments
- Preterm Birth and Chorioamnionitis
Hektoen Institute
2021
Rush University Medical Center
2007-2016
Rush University
1999-2014
Presbyterian St. Luke's Medical Center
1993-2013
Loyola University Chicago
2011
University of Illinois Chicago
1984-2007
St. Luke's Hospital
2005
Frontier Science & Technology Research Foundation
2001
University of Copenhagen
2000
National Institutes of Health
1999
Objective Lactobacillus dominates the lower genital tract microbiota of many women, producing a low vaginal pH, and is important for healthy pregnancy outcomes protection against several sexually transmitted pathogens. Yet, factors that promote remain poorly understood. We hypothesized amount free glycogen in lumen an determinant colonization pH. Methods Free lavage samples was quantified. Pyrosequencing 16S rRNA gene used to identify from 21 African American women collected over 8–11 years....
Lactobacillus colonization of the lower female genital tract provides protection from acquisition sexually transmitted diseases, including human immunodeficiency virus, and adverse pregnancy outcomes. While glycogen in vaginal epithelium is thought to support vivo, many isolates cannot utilize vitro. This study investigated how could be utilized by lactobacilli tract. Several were confirmed not grow glycogen, but did glycogen-breakdown products, maltose, maltotriose, maltopentaose,...
Bacterial vaginosis (BV) is associated with human immunodeficiency virus (HIV) acquisition. We examined the association between BV and BV-associated bacteria expression of HIV in female genital tract.HIV RNA, lactobacilli, Gardnerella vaginalis, Mycoplasma hominis cervicovaginal lavage (CVL) samples were quantified by polymerase chain reaction (PCR). Gynecologic evaluation included Nugent score assessment, Amsel criteria detection other genital-tract infections, dysplasia grading. CD4 cell...
This study investigates whether cell-derived glycosylphosphatidylinositol-linked complement control proteins CD55 and CD59 can be incorporated into HIV-1 virions contribute to resistance. Virus was prepared by transfection of cell lines with pNL4-3, primary isolates were derived from patients' PBMCs. tested for sensitivity complement-mediated virolysis in the presence anti-gp160 antibody. Viral preparations JY33 cells, which lack CD59, highly sensitive complement. H9 U937 express low levels...
Neuroleukin is a lymphokine product of lectin-stimulated T cells that induces immunoglobulin secretion by cultured human peripheral blood mononuclear cells. acts early in the vitro response leads to formation antibody-secreting cells, but continued production differentiated neuroleukin-independent. Although factor not directly mitogenic, cellular proliferation late component neuroleukin. does have B-cell growth (BCGF) or differentiation (BCDF) activity defined assays. Neuroleukin-evoked...
ABSTRACT Bacterial vaginosis (BV) is a clinical syndrome presenting with malodorous vaginal discharge and increased pH. Diagnosis has been based on Amsel criteria direct Gram stain of secretions. Human immunodeficiency virus (HIV)-infected participants in the Women's Interagency HIV Study contributed cervicovaginal lavage (CVL) samples. Lactobacilli, Gardnerella vaginalis , Mycoplasma hominis samples were quantified by PCR. Gynecologic evaluation included Nugent score criterion assessment....
Whether human immunodeficiency virus (HIV) infection is associated with a change in the diversity of genital microbiota women was investigated.Amplicon length heterogeneity polymerase chain reaction (LH-PCR) analysis and pyrosequencing 16S ribosomal RNA gene were used to analyze HIV-positive (HIV(+)) HIV-negative (HIV(-)) or without bacterial vaginosis (BV).LH-PCR revealed significantly more BV-positive (BV(+)) than BV-negative (BV(-)) women, but no significant difference noted between...
Background Colonization of the female lower genital tract with Lactobacillus provides protection against STIs and adverse pregnancy outcomes. Growth is postulated to depend on epithelial cell-produced glycogen. However, amount cell-free glycogen in fluid available for utilization by not known. Methods Eighty-five samples from 7 pre-menopausal women taken over 4–6 weeks were obtained using Instead SoftCup® (EvoFem, Inc., San Diego, CA, USA) consented donors. Cell-free glucose fluids estrogen...
Human immunodeficiency virus type 1 (HIV-1) can be either resistant or sensitive to complement- mediated destruction depending on the host cells. Incorporation of different levels cell CD46, CD55 and CD59 may account for this differential sensitivity complement. However, it has not been determined whether all incorporated at which protect virions. To determine each these proteins HIV-1, virions were derived from CHO cells expressing human CD59. Virions shown incorporate both glycosyl...
The current study was undertaken to determine whether the human T cell leukemia/lymphoma oncovirus type I (HTLV-I) and herpesvirus cytomegalovirus (HCM) incorporate host cell-derived C regulatory proteins. Our experiments showed that both CD59 CD55 were associated with external membrane of HTLV-I derived from MT2 cells, since virus could be captured by mAbs these proteins, antisera induced C-mediated lysis virions. Additionally, detected immunoblot analysis purified HTLV-I. Purified HCMV...
Mannose-binding lectin (MBL) is present in human serum and plays an important role innate immunity by binding to carbohydrate on micro-organisms. Whereas the gp120/gp41 of immunodeficiency virus type 1 (HIV-1) contains numerous N-linked glycosylation sites many these contain high-mannose glycans which could interact with MBL, interaction between MBL primary isolates (PI) HIV-1 has not been studied. To determine if PI HIV bind a capture assay was developed incubated MBL-coated microtitre...
Mannose-binding lectin (MBL), a serum that mediates innate immune functions including activation of the complement pathway, binds to carbohydrates expressed on some viral glycoproteins. In this study, ability MBL bind virus particles pseudotyped with Ebola and Marburg envelope glycoproteins was evaluated. Virus bearing either (Zaire strain) or (Musoke bound at significantly higher levels immobilized compared vesicular stomatitis glycoprotein no glycoprotein. As observed in previous studies,...
Mannose-binding lectin (MBL) targets diverse microorganisms for phagocytosis and complement-mediated lysis by binding specific surface glycans. Although recombinant human MBL (rhMBL) trials have focused on reconstitution therapy, safety studies identified no barriers to its use at higher levels. Ebola viruses cause fatal hemorrhagic fevers which treatment exists that are feared as potential biothreat agents. We found mice whose rhMBL serum concentrations were increased ≥7-fold above average...
Mannose-binding lectin (MBL) is a key soluble effector of the innate immune system that recognizes pathogen-specific surface glycans. Surprisingly, low-producing MBL genetic variants may predispose children and immunocompromised individuals to infectious diseases are more common than would be expected in human populations. Since certain defense molecules, such as immunoglobulins, can exploited by invasive pathogens, we hypothesized might also enhance infections some circumstances....
Bacterial vaginosis (BV) is characterized by low abundance of Lactobacillus species, high pH, and immune cell infiltration has been associated with an increased risk human papillomavirus (HPV) infection. We molecularly assessed the cervicovaginal microbiota over time in immunodeficiency virus (HIV)-infected HIV-uninfected women to more comprehensively study HPV-microbiota relationship, controlling for status. 16S ribosomal RNA gene amplicon pyrosequencing HPV DNA testing were conducted...
This study was undertaken to directly assess the susceptibility of HIV-1 plasma virus C-mediated lysis. Plasma from HIV-infected individuals collected and ultracentrifuged over 20% sucrose isolate virions components including anticoagulants, which inhibit C activity. Treatment with alone in absence exogenously added Ab caused lysis all patients (n = 18) (range 14 86%). occurred via classical pathway not due cross-reactive Abs source. Protein A bound a fraction isolated this binding blocked...
The aim of this study was to determine the feasibility detecting bacterial vaginosis (BV)-related organisms in stored genital tract specimens using real-time PCR. Frozen cervicovaginal lavage (CVL) samples from 21 women were analyzed by PCR for numbers Mycoplasma hominis, Gardnerella vaginalis and lactobacilli. Lactobacilli detected all CVL samples, G. but one sample, while M. hominis only six samples. Using Amsel criteria define BV, with BV had significantly higher than without (P=0.004)....
Innate immune factors in mucosal secretions may influence human immunodeficiency virus type 1 (HIV-1) transmission. This study examined the levels of three such factors, genital tract lactoferrin [Lf], secretory leukocyte protease inhibitor [SLPI], and RANTES, women at risk for acquiring HIV infection, as well cofactors that be associated with their presence. Women high infection meeting established criteria (n = 62) low-risk controls 33) underwent cervicovaginal lavage (CVL), CVL fluid...
The ability of complement to inactivate human immunodeficiency virus (HIV) in the presence specific antibody was evaluated. HIV treated with and/or antibody, and then its titer determined on CD4+ H9 cell line. While alone had no effect titer, plus subneutralizing levels resulted reductions. Complement sources deficient membrane attack component C5 or C8 did not antibody-treated HIV, suggesting that neutralization occurred via lysis. This possibility investigated by assessing release reverse...
The composition of the lower genital tract microbiota in women is believed to affect risk sexually acquiring HIV. Since macaque could similarly impact vaginal infection with SIV we identified 11 rhesus macaques using multitag pyrosequencing 16S rRNA gene. was polymicrobial a median nine distinct bacterial taxa per (range 3–16 taxa, each constituting 1% or more sequences). Taxa frequently found included Peptoniphilus, Sneathia, Porphyromonas, Mobiluncus, Atopobacter, Dialister, Thioreductor,...
Understanding factors that affect heterosexual transmission of HIV in women is great importance. Lactobacilli the lower genital tract utilize glycogen vaginal epithelial cells as an energy source and produce lactic acid. The resultant acidity believed to provide protection against infection. Conversely, bacterial vaginosis (BV) characterized by less acid a higher pH, associated with increased susceptibility Because infection macaques simian immunodeficiency virus (SIV) or simian-human (SHIV)...
Bacterial vaginosis (BV) is associated with an increased rate of sexual transmission human immunodeficiency virus (HIV) type 1, and Gardnerella vaginalis frequently isolated from the genital tracts women BV. G. lysates were found to significantly stimulate HIV expression in monocytoid cells. Stimulation was higher when heated at 100°C for 5 min but reduced by treatment lysozyme or protease. also activated certain T cell lines. long-terminal repeat transcription HIV-infected cells NF-κB...