A5011591486- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Smoking Behavior and Cessation
- Obesity, Physical Activity, Diet
- Infective Endocarditis Diagnosis and Management
- CAR-T cell therapy research
- Streptococcal Infections and Treatments
- Health disparities and outcomes
- Bacterial Genetics and Biotechnology
- Cardiac Valve Diseases and Treatments
- Prion Diseases and Protein Misfolding
- Animal Disease Management and Epidemiology
- Cardiovascular Health and Risk Factors
- Pulmonary Hypertension Research and Treatments
- Cardiac tumors and thrombi
- Advanced biosensing and bioanalysis techniques
- Pediatric health and respiratory diseases
- Peptidase Inhibition and Analysis
- Heart Failure Treatment and Management
- bioluminescence and chemiluminescence research
University of Hawaiʻi at Mānoa
2023-2024
University of Hawaii System
2023-2024
Queen's Medical Center
2021-2024
Hamilton Medical Center
2023
Queens Hospital Center
2021
George Mason University
2004-2019
University of Hawaii at Hilo
2018
Amherst College
2015-2016
Cornell University
2003
University of Washington
1995-1998
Novel human adenoviruses (HAdVs) arise from genome recombination. Analysis of HAdV type 55 an outbreak in China shows a hexon recombination between HAdV-B11 and HAdV-B14, resulting that is 97.4% HAdV-B14. Sporadic appearances as re-emergent pathogen misidentification "HAdV-B11a" are due to this partial hexon.
The prion protein (PrP), first identified in scrapie-infected rodents, is encoded by a single exon of single-copy chromosomal gene. In addition to the protein-coding exon, PrP genes mammals contain one or two 5′-noncoding exons. To learn more about genomic organization regions surrounding exons, we sequenced 10 5 bp DNA from clones containing human, sheep, and mouse isolated cosmids λ phage. Our findings are as follows: (1) Although human transcript does not include untranslated 2 found its...
Human adenovirus C (HAdV-C) species are a common cause of respiratory infections and can occasionally produce severe clinical manifestations. A deeper understanding the variation evolution in HAdV-C is especially important since these viruses, including HAdV-C6, used as gene delivery vectors for human therapy other biotechnological applications. Here, full-genome analysis prototype HAdV-C6 recently identified virus provisionally termed HAdV-C57 reported. Although genomes all members very...
Human adenoviruses (HAdVs) are highly contagious pathogens causing acute respiratory disease (ARD), among other illnesses. Of the ARD genotypes, HAdV-7 presents with more severe morbidity and higher mortality than others. We report isolation identification of a genome type HAdV-7d (DG01_2011) from recent outbreak in Southern China. Genome sequencing, phylogenetic analysis restriction endonuclease (REA) comparisons past indicate has re-emerged China after an absence twenty-one years....
Extremely halophilic archaea, which flourish in hypersaline environments, are known to contain a variety of large dynamic replicons. Previously, the analysis one such replicon, pNRC100, Halobacterium sp. strain NRC-1, showed that it undergoes high-frequency insertion sequence (IS) element-mediated insertions and deletions, as well inversions via recombination between 39-kb-long inverted repeats (IRs). Now, complete sequencing 191,346-bp circle, has shown presence 27 IS elements representing...
Human adenoviruses (HAdVs) are uniquely important "model organisms" as they have been used to elucidate fundamental biological processes, recognized complex pathogens, and remedies for human health. As HAdVs may effect asymptomatic or mild severe symptomatic disease upon their infection of respiratory, ocular, gastrointestinal, genitourinary systems. High-resolution genomic data enhanced the understanding HAdV epidemiology, with recombination an major pathway in molecular evolution genesis...
In November of 2007 a human adenovirus (HAdV) was isolated from bronchoalveolar lavage (BAL) sample recovered biopsy an AIDS patient who presented with fever, cough, tachycardia, and expiratory wheezes. To better understand the virus, genome sequenced analyzed using bioinformatic phylogenomic analysis. The results suggest that this novel which is provisionally named HAdV-D59, may have been created multiple recombination events. Specifically, penton, hexon, fiber genes high nucleotide...
Genomics analysis of a historically intriguing and predicted emergent human adenovirus (HAdV) pathogen, which caused pneumonia death, provides insight into novel molecular evolution pathway involving "ping-pong" zoonosis anthroponosis. The genome this promiscuous pathogen is embedded with evidence unprecedented multiple, multidirectional, stable, reciprocal cross-species infections hosts from three species (human, chimpanzee, bonobo). This recombinant genome, typed as HAdV-B76, identical to...
ABSTRACT Vaccine strains of human adenovirus serotypes 4 and 7 (HAdV-4vac HAdV-7vac) have been used successfully to prevent adenovirus-related acute respiratory disease outbreaks. The genomes these two vaccine sequenced, annotated, compared with their prototype equivalents the goals understanding for molecular diagnostics applications, redevelopment, HAdV pathoepidemiology. These reference are archived in GenBank as HAdV-4vac (35,994 bp; AY594254 ) HAdV-7vac (35,240 AY594256). Bioinformatics...
Homo sapiens possess several trypsinogen or trypsinogen-like genes of which three (PRSS1, PRSS2, and PRSS3) produce functional trypsins in the digestive tract. PRSS1 PRSS2 are located on chromosome 7q35, while PRSS3 is found 9p13. Here, we report a variation theme new gene creation by duplication: was formed segmental duplications originating from chromosomes 7q35 11q24. As result, transcripts display two variants exon 1. The transcript whose organization most resembles encodes protein...
The 36,001 base pair DNA sequence of human adenovirus serotype 1 (HAdV-1) has been determined, using a 'leveraged primer sequencing strategy' to generate high quality sequences economically. This annotated genome (GenBank AF534906) confirms anticipated similarity closely related species C (formerly subgroup), adenoviruses HAdV-2 and -5, near identity with earlier reports representing parts the HAdV-1 genome. A first round data acquisition used PCR amplification primers from common genomes...
ABSTRACT Five genomes of human subspecies B1 adenoviruses isolated from cases acute respiratory disease have been sequenced and archived for reference. These include representatives two prevalent genomic variants HAdV-7, i.e., HAdV-7h HAdV-7d2. The other three are HAdV-3/16, HAdV-16 strain E26, HAdV-3+7 Takeuchi. All recombinant genomes. Genomics bioinformatics provide detailed views into the genetic makeup these pathogens insight their molecular evolution. Retrospective characterization...
Technological advances and increasingly cost-effect methodologies in DNA sequencing computational analysis are providing genome proteome data for human adenovirus research. Applying these tools, derived knowledge to the development of vaccines against pathogens will provide effective prophylactics. The same approaches can be applied vector gene delivery therapy vaccine protocols. Examination several field strain genomes their analyses examples that available using approaches. An example...