A5014012352- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Autoimmune and Inflammatory Disorders Research
- Psoriasis: Treatment and Pathogenesis
- Advancements in Transdermal Drug Delivery
- Toxin Mechanisms and Immunotoxins
- Spondyloarthritis Studies and Treatments
- Galectins and Cancer Biology
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Hormonal Regulation and Hypertension
- Bioactive Natural Diterpenoids Research
- Cardiac Fibrosis and Remodeling
- Lipoproteins and Cardiovascular Health
- Advanced Biosensing Techniques and Applications
- Plant-based Medicinal Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- Ferroptosis and cancer prognosis
Osaka University
2018-2024
First Affiliated Hospital of Xinjiang Medical University
2021-2024
Xinjiang Medical University
2021-2024
Shandong Provincial Hospital
2019-2022
Second Hospital of Shandong University
2020-2021
Qilu Hospital of Shandong University
2018-2019
Senotherapy targeting for senescent cells is designed to attenuate age-related dysfunction. Senescent T cells, defined as CD4+ CD44high CD62Llow PD-1+ CD153+ accumulate in visceral adipose tissues (VAT) obese individuals. Here, we show the long-lasting effect of using CD153 vaccination remove from high-fat diet (HFD)-induced C57BL/6J mice. We administered a peptide-KLH (keyhole limpet hemocyanin) conjugate vaccine with Alhydrogel (CD153-Alum) or CpG oligodeoxynucleotide (ODN) 1585...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates expression of low-density lipoprotein (LDL) receptors via receptor internalization and subsequent lysosomal degradation. Thus, an anti-PCSK9 antibody is well known as anti-hyperlipidemia drug. Here, we aimed to develop vaccine for a long-term treatment dyslipidemia targeted PCSK9. In This study, designed peptide mouse PCSK-9, which consisted short peptides conjugated keyhole limpet hemocyanin (KLH) carrier protein. Vaccines were...
This study was designed to investigate the correlation of neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte (PLR), and interleukin (IL)-37/IL-17 with incidence/treatment rheumatoid arthritis (RA). Firstly, fifty-eight patients RA treated at first affiliated hospital Xinjiang Medical University from January 2018 2019 were selected as group; forty-nine healthy volunteers enrolled in control group. disease-modifying anti-rheumatic drugs (DMARDs). Next, NLR, PLR, IL-37, IL-17 28-joint...
DNA vaccination can be applied to the treatment of various infectious diseases and cancers; however, technical difficulties have hindered development an effective delivery method. The efficacy a vaccine depends on optimal antigen expression by injected plasmid DNA. pyro-drive jet injector (PJI) is novel system that allows for adjustment injection depth may, thus, provide targeted approach therapeutic or preventative compounds. Herein, we investigated its potential use in delivering vaccines....
BACKGROUND Anti-inflammatory mediators such as mucin-domain containing-3 (TIM-3) and IL-37 play an important role in the regulation of Th1-mediated immunity. This study was designed to investigate proportions various T cell subsets monocytes peripheral blood rheumatoid arthritis (RA) patients, well level TIM-3 on these cells serum cytokine levels. MATERIAL AND METHODS We enrolled 59 RA patients 46 age- sex-matched healthy controls this study. The proportion expression were determined by flow...
We conducted a nonrandomized, open-label phase I study to assess the safety and immunogenicity of an intradermal coronavirus disease 2019 (COVID-19) DNA vaccine (AG0302-COVID-19) administered using pyro-drive jet injector at Osaka University Hospital between Yanagida November 2020 December 2021. Twenty healthy volunteers, male or female, were enrolled in low-dose (0.2 mg) high-dose (0.4 groups AG0302-COVID19 twice 2-week interval. There no adverse events that led discontinuation drug...
BACKGROUND: Myofibroblasts are primary cells involved in chronic response-induced cardiac fibrosis. Fibroblast activation protein (FAP) is a relatively specific marker of activated myofibroblasts and potential target molecule. This study aimed to clarify whether vaccine targeting FAP could eliminate stress model mice reduce METHODS: We coadministered peptide with CpG K3 oligonucleotide adjuvant male C57/BL6J confirmed an elevation the anti-FAP antibody titer. After continuous angiotensin II...
There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through development of efficient and safe vaccination methods. Over short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport storage. pVAX1-SARS-CoV2-co was designed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led immunoreactions against protein, anti-receptor-binding-domain, a neutralizing action...
The aim of this study was to investigate the effect Iguratimod (T-614) on rat knee osteoarthritis (KOA) and further explore its underlying mechanism.In study, papain-induced KOA model constructed. Hematoxylin eosin (H&E) staining conducted observe pathological changes cartilage tissue Mankin scoring principle used for quantitative scoring. Transmission electron microscopy (TEM) applied ultrastructure tissue. ELISA measure levels matrix metalloproteinase 13 (MMP-13) inflammatory factors...
We previously reported that silencing of the PRR gene, which encodes (pro)renin receptor [(P)RR], significantly reduced Wnt/β-catenin-dependent development pancreatic ductal adenocarcinoma (PDAC). Here, we examined effects a panel blocking mAbs directed against (P)RR extracellular domain on proliferation human PDAC cell lines PK-1 and PANC-1 in vitro vivo observed four rat anti-(P)RR induced accumulation cells G0-G1-phase cycle concomitant with an attenuation Wnt/β-catenin signaling....
There is an urgent need to limit and stop the worldwide coronavirus disease 2019 (COVID-19) pandemic via quick development of efficient safe vaccination methods. Plasmid DNA vaccines are one most remarkable that can be developed in a short term. pVAX1-SARS-CoV2-co, which plasmid vaccine, was designed express severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. The produced antibodies lead Immunoreactions against S protein, anti-receptor-binding-domain, neutralizing...
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global pandemic. New technologies have been utilized develop several types of vaccines prevent the spread SARS-CoV-2 infection, including mRNA vaccines. Our group previously developed an effective DNA-based vaccine. However, emerging variants concern (VOCs), such as delta variant, escaped mutations against vaccine-induced neutralizing antibodies. This suggests that...
Abstract Emerging SARS-CoV-2 Omicron variants are highly contagious with enhanced immune escape mechanisms against the initially approved COVID-19 vaccines. Therefore, we require stable alternative-platform vaccines that confer protection newer of SARS-CoV-2. We designed an B.1.1.529 specific DNA vaccine using our platform and evaluated humoral cellular responses. SD rats intradermally administered Omicron-specific via pyro-drive jet injector (PJI) thrice at 2-week intervals elicited high...
Objectives Spondyloarthritis (SpA) is known as series of immune-mediated inflammatory disease the axial and peripheral joints. Human leucocyte antigen (HLA)-B27 a genetic risk factor for SpA. Recent evidence suggests that interleukin −17 (IL17) axis strongly contributes to This study aimed assess efficacy an IL17A peptide-based vaccine on SpA manifestations in model rats. Methods HLA-B27/human β 2 -microglobulin (hβ M) transgenic rats were immunised with heat-inactivated Mycobacterium...
Rheumatoid arthritis (RA) is an autoinflammatory disease, its core treatment principle to achieve remission as soon possible. There no good prediction model that can accurately predict the rate of patients choose a scheme. Here, we aimed verify prognostic value some inflammatory indicators in RA and establish after treatment.A total 223 were enrolled at Qilu Hospital from June 2014 2020. Baseline clinical data collected plasma was obtained detect indicators. All treated with conventional...
Objectives: We recently developed a novel peptide, AJP001, that possesses both mouse T-cell epitope and adjuvant action. Direct conjugation to the antigen is useful for peptide vaccines without addition of adjuvants. In this study, efficacy an angiotensin (Ang) II AJP001-conjugated vaccine (AJ-Ang II) was evaluated in mice. Methods: The anti-Ang antibody titer measured Balb/C mice following three injections AJ-Ang at 2-week intervals. SBP during vaccination treated with Ang infusion (1 μg/kg...
Abstract To fight against the worldwide COVID-19 pandemic, development of an effective and safe vaccine SARS-CoV-2 is required. As potential pandemic vaccines, DNA/RNA viral vector vaccines protein-based have been rapidly developed to prevent spread worldwide. In this study, we designed plasmid DNA targeting Spike glycoprotein (S protein) as vaccine, humoral, cellular, functional immune responses were characterized support proceeding initial human clinical trials. After intramuscular...
ABSTRACT Rheumatoid arthritis (RA), a highly disabling autoimmune disease, is characterized by joint damage and synovial hyperplasia. This paper was designed to explore the effect mechanisms of Aconitum diphtheria on proliferation apoptosis RA fibroblast‐like synoviocytes (RA‐FLS). First, RA‐FLS treated with different doses A. extracts. Then, an inverted biological microscope adopted observe morphology. Subsequently, terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end...
<h3>Background</h3> Ankylosing spondylitis (AS) is one of the major diseases in spondyloarthritis (SpA). HLA-B27 known as a genetic risk factor for AS. Recent lines evidence suggests that IL-23/IL-17 axis strongly contributes to <h3>Objectives</h3> This study was assess efficacy IL17A peptide-based vaccine on SpA manifestations model rat. <h3>Methods</h3> HLA-B27/human beta 2 -microglobulin transgenic rats were immunized with heat-inactivated Mycobacterium Tuberculosis (MT) develop and...