A5078252717- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- Animal Disease Management and Epidemiology
- bioluminescence and chemiluminescence research
- Vector-borne infectious diseases
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- Rabies epidemiology and control
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- Studies on Chitinases and Chitosanases
- Immune responses and vaccinations
Institut Pasteur
2022-2025
Osaka University
2020-2024
Université Paris Cité
2024
Centre National de la Recherche Scientifique
2024
Hokkaido University
2014-2023
Department of Virology
2022-2023
Sapporo Science Center
2019
Many SARS-CoV-2 variants with naturally acquired mutations have emerged. These can affect viral properties such as infectivity and immune resistance. Although the sensitivity of occurring to humoral immunity has been investigated, human leukocyte antigen (HLA)-restricted cellular remains largely unexplored. Here, we demonstrate that two recently emerging in receptor-binding domain spike protein, L452R (in B.1.427/429 B.1.617) Y453F B.1.1.298), confer escape from HLA-A24-restricted immunity....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of disease 2019 (COVID-19). Although multiple mutations have observed in SARS-CoV-2, functional analysis each mutation SARS-CoV-2 limited by lack convenient mutagenesis methods. In this study, we establish a PCR-based, bacterium-free method to generate infectious clones. Recombinant could be rescued at high titer with accuracy after assembling 10 cDNA fragments circular polymerase...
Summary During the current SARS-CoV-2 pandemic that is devastating modern societies worldwide, many variants naturally acquire multiple mutations have emerged. Emerging can affect viral properties such as infectivity and immune resistance. Although sensitivity of occurring to humoral immunity has recently been investigated, human leukocyte antigen (HLA)-restricted cellular remains unaddressed. Here we demonstrate two emerging mutants in receptor binding domain spike protein, L452R (in...
Abstract Reports of post-acute COVID-19 syndrome, in which the inflammatory response persists even after SARS-CoV-2 has disappeared, are increasing 1 , but underlying mechanisms syndrome remain unknown. Here, we show that SARS-CoV-2-infected cells trigger senescence-like cell-cycle arrest 2,3 neighboring uninfected a paracrine manner via virus-induced cytokine production. In cultured human or bronchial organoids, these SASR-CoV-2 infection-induced senescent express high levels series factors...
The widespread occurrence of SARS-CoV-2 has had a profound effect on society and vaccine is currently being developed. Angiotensin-converting enzyme 2 (ACE2) the primary host cell receptor that interacts with receptor-binding domain (RBD) spike protein. Although pneumonia main symptom in severe cases infection, expression levels ACE2 lung low, suggesting presence another for In order to identify additional receptors protein, we screened protein from cDNA library. We cloned L-SIGN as specific...
The emergence of novel tick-borne RNA viruses causing severe illness in humans has complicated the epidemiological landscape diseases, requiring further investigation to safeguard public health. In present study, we discovered a phlebovirus from Ixodes persulcatus ticks Japan. While its viral genome sequences were similar those mosquito/sandfly-borne viruses, molecular and biological footprints confirmed that this is virus. unique evolutionary position virus allowed us estimate ancestral...
In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b were predominant. the present 766 BVDVs 2014 Hokkaido, Japan, understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of 5′-untranslated region genome revealed isolates classified as genotype 1 (BVDV-1; 544 isolates) 2 (BVDV-2; 222). BVDV-1 further divided into BVDV-1a (93), (371) 1c (80) subgenotypes, all BVDV-2...
Mutations continue to accumulate within the SARS-CoV-2 genome, and ongoing epidemic has shown no signs of ending. It is critical predict problematic mutations that may arise in clinical environments assess their properties advance quickly implement countermeasures against future variant infections. In this study, we identified resistant remdesivir, which widely administered SARS-CoV-2-infected patients, discuss cause resistance. First, simultaneously constructed eight recombinant viruses...
Monitoring
Viral protein assembly and virion budding are tightly regulated to enable the proper formation of progeny virions. At this late stage in virus life cycle, some enveloped viruses take advantage host endosomal sorting complex required for transport (ESCRT) machinery, which contributes physiological functions membrane modulation abscission. Bullet-shaped viral particles unique morphological characteristics rhabdoviruses; however, involvement factors rhabdovirus infection and, specifically,...
Zika virus (ZIKV) is a mosquito-borne flavivirus primarily transmitted among humans by Aedes aegypti . Over the past two decades, it has caused significant outbreaks associated with birth defects and neurological disorders. Phylogenetically, ZIKV consists of main genotypes referred to as African Asian lineages, each exhibiting distinct biological properties. lineage strains are more efficiently mosquitoes, but pinpointing genetic basis this difference remained challenging. Here, we address...
Live-attenuated vaccines are generally highly effective. Here, we aimed to develop one against SARS-CoV-2, based on the identification of three types temperature-sensitive (TS) strains with mutations in nonstructural proteins (nsp), impaired proliferation at 37°C–39°C, and capacity induce protective immunity Syrian hamsters. To a live-attenuated vaccine, generated virus that combined all these TS-associated (rTS-all), which showed robust TS phenotype vitro high attenuation vivo. The vaccine...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes several host proteases to cleave the spike (S) protein enter cells. SARS-CoV-2 S is cleaved into S1 and S2 subunits by furin, which closely involved in pathogenicity of SARS-CoV-2. However, effects modulated protease cleavage activity due mutations on viral replication pathogenesis remain unclear. Herein, we serially passaged two strains Vero cells characterized cell-adapted vitro vivo . The adapted showed high...
Abstract Zika virus (ZIKV), an emerging mosquito-borne flavivirus, is associated with congenital neurological complications. Here, we investigate potential pathological correlates of gene expression in representative ZIKV strains through RNA sequencing and ribosome profiling. In addition to the single long polyprotein found all flaviviruses, identify translation unrecognised upstream open reading frames (uORFs) genomic 5′ region. Asian/American strains, ribosomes translate uORF1 uORF2,...
Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), member of the
The discovery of novel antivirals to treat hepatitis B virus (HBV) infection is urgently needed, as the currently available drugs mainly target viral proteins at replication step, whereas host factors also play significant roles in HBV infection. Although numerous studies have reported candidate for treatment, there remains a need find new drug that may other steps life cycle. In this study, by screening 533 G-protein-coupled receptors (GPCRs)-associated compound library, we identified...
There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through development of efficient and safe vaccination methods. Over short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport storage. pVAX1-SARS-CoV2-co was designed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led immunoreactions against protein, anti-receptor-binding-domain, a neutralizing action...
ABSTRACT Although the global crisis caused by coronavirus disease 2019 (COVID-19) pandemic is over, epidemic of continues. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), cause COVID-19, initiates infection via binding receptor-binding domain (RBD) its spike protein to human angiotensin-converting enzyme II (ACE2) receptor, and this interaction has been primary target for development COVID-19 therapeutics. Here, we identified neutralizing antibodies against SARS-CoV-2 screening...
Abstract SARS-CoV-2 infection causes severe symptoms in a subset of patients, suggesting the presence certain unknown risk factors. Although antibodies against receptor-binding domain (RBD) spike have been shown prevent infection, effects other protein domains are largely unknown. Here, we screened series anti-spike monoclonal from COVID-19 and found that some N-terminal (NTD) dramatically enhanced binding capacity to ACE2, thus increased SARS-CoV2 infectivity. Surprisingly, mutational...
BackgroundFrom late March through April 2021, we experienced a cluster of patients with COVID-19, named "Cluster K", rapid severe illness compared those who were infected before.MethodsPatients COVID-19 enrolled in this study divided into two groups: 66 from November 2020 to 2021 (group A) and 37 whose infection links traced Cluster K B). The primary outcome was mortality rate, the secondary maximal oxygen flow rate as severity disease. Viral genome sequences between groups.ResultsMortality...
There is an urgent need to limit and stop the worldwide coronavirus disease 2019 (COVID-19) pandemic via quick development of efficient safe vaccination methods. Plasmid DNA vaccines are one most remarkable that can be developed in a short term. pVAX1-SARS-CoV2-co, which plasmid vaccine, was designed express severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. The produced antibodies lead Immunoreactions against S protein, anti-receptor-binding-domain, neutralizing...