A5055552571- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Microfluidic and Bio-sensing Technologies
- COVID-19 Clinical Research Studies
- Microbial Inactivation Methods
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Cancer Immunotherapy and Biomarkers
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Acute Ischemic Stroke Management
- Inhalation and Respiratory Drug Delivery
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- Advancements in Transdermal Drug Delivery
Osaka University
2016-2023
// Chin Yang Chang 1 , Jiayu A. Tai Sumin Li Tomoyuki Nishikawa Yasufumi Kaneda Division of Gene Therapy Science, Graduate School Medicine, Osaka University, Suita, 565-0871, Japan Correspondence to: Kaneda, email: kaneday@gts.med.osaka-u.ac.jp Keywords: HVJ-E, tumor associated neutrophils (TANs), anti-tumor immunity, microenvironment (TME), cytotoxic T lymphocytes (CTLs) Received: November 20, 2015 Accepted: May 22, 2016 Published: 31, ABSTRACT The (TME) fosters tumors by attenuating...
There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through development of efficient and safe vaccination methods. Over short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport storage. pVAX1-SARS-CoV2-co was designed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led immunoreactions against protein, anti-receptor-binding-domain, a neutralizing action...
Cell-cell fusion involves the of somatic cells into a single hybrid cell. It is not only physiological process but also an important cell engineering technology which can be applied to various fields, such as regenerative medicine, antibody engineering, genetic and cancer therapy. There are three major methods fusion: electrical fusion, polyethylene glycol (PEG) virus-mediated fusion. Although PEG most economical approach does require expensive instrumentation, it has poor rate induces high...
Breast cancer has a high risk of metastasis; however, no effective treatment been established. We developed novel immunotherapy for breast to enhance cytotoxic T lymphocytes against cells using N1-type neutrophils with anti-tumor properties. For this purpose, we combined CXCL2 (CXC chemokine ligand 2) plasmid DNA inactivated Sendai virus (hemagglutinating Japan)-envelope (HVJ-E). The combination and HVJ-E (C/H) suppressed the growth murine cancers in orthotopic syngeneic models by enhancing...
There is an urgent need to limit and stop the worldwide coronavirus disease 2019 (COVID-19) pandemic via quick development of efficient safe vaccination methods. Plasmid DNA vaccines are one most remarkable that can be developed in a short term. pVAX1-SARS-CoV2-co, which plasmid vaccine, was designed express severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. The produced antibodies lead Immunoreactions against S protein, anti-receptor-binding-domain, neutralizing...
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global pandemic. New technologies have been utilized develop several types of vaccines prevent the spread SARS-CoV-2 infection, including mRNA vaccines. Our group previously developed an effective DNA-based vaccine. However, emerging variants concern (VOCs), such as delta variant, escaped mutations against vaccine-induced neutralizing antibodies. This suggests that...
Abstract Emerging SARS-CoV-2 Omicron variants are highly contagious with enhanced immune escape mechanisms against the initially approved COVID-19 vaccines. Therefore, we require stable alternative-platform vaccines that confer protection newer of SARS-CoV-2. We designed an B.1.1.529 specific DNA vaccine using our platform and evaluated humoral cellular responses. SD rats intradermally administered Omicron-specific via pyro-drive jet injector (PJI) thrice at 2-week intervals elicited high...
Recent advances in endovascular treatment (EVT) dramatically changed the outcome of ischemic stroke, but partial reperfusion does not improve as well no reperfusion. Although is estimated to be more potential for therapeutic intervention than permanent occlusion due some blood supply, their pathophysiological difference still unknown. To answer question, we analyzed mice, which were exposed distal middle cerebral artery with 14-min common carotid (CCA) (partial reperfusion) or CCA (no...
Neutrophils are the first line of defense against pathogens in immune system, and also has important implications for tumor micro-environment. The effect neutrophils on cancer is a double-edged sword that they can both promote or inhibit growth. When different environments exposed to stimulation, would polarize toward anti-tumorigenic N1 pro-tumorigenic N2 phenotype. exact mechanism not yet well understood, but it currently known IFN-β stimulation pathogen-associated molecular patterns...