A5086663538- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Enzyme Production and Characterization
- Biochemical and Structural Characterization
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Asthma and respiratory diseases
- Advanced Proteomics Techniques and Applications
- COVID-19 Clinical Research Studies
- Phytase and its Applications
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Protein Hydrolysis and Bioactive Peptides
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Cell death mechanisms and regulation
- IL-33, ST2, and ILC Pathways
- Mast cells and histamine
UCSI University
2015-2024
Max Planck Institute for Multidisciplinary Sciences
2022-2024
University of Salzburg
2017-2023
Osaka International University
2020-2021
Osaka University
2020-2021
Chulalongkorn University
2015-2016
Understanding patterns of IgE sensitization in Dermatophagoides-allergic patients living various geographical areas is necessary to design a product suitable for worldwide allergen immunotherapy (AIT).Using HIFI Allergy customized microarray assay, IgEs specific 12 purified allergens from Dermatophagoides pteronyssinus or D. farinae were assessed sera 1302 house dust mite (HDM)-allergic areas. Comprehensive mass spectrometric (MS) analyses conducted characterize HDM extracts, as well bodies...
Abstract If and how proteasomes catalyze not only peptide hydrolysis but also splicing is an open question that has divided the scientific community. The debate so far been based on immunopeptidomics, in vitro digestions of synthetic polypeptides as well ex vivo experiments, which could indirectly describe proteasome-catalyzed full-length proteins. Here we develop a workflow—and cognate software - to analyze proteasome-generated non-spliced spliced peptides produced from entire proteins...
The widespread occurrence of SARS-CoV-2 has had a profound effect on society and vaccine is currently being developed. Angiotensin-converting enzyme 2 (ACE2) the primary host cell receptor that interacts with receptor-binding domain (RBD) spike protein. Although pneumonia main symptom in severe cases infection, expression levels ACE2 lung low, suggesting presence another for In order to identify additional receptors protein, we screened protein from cDNA library. We cloned L-SIGN as specific...
Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major allergen. However, molecular mechanisms of allergic sensitization, including roles and other components extract, remain incompletely understood. Here, we examined how known pollen-derived molecules influence endolysosomal processing 1, thereby shaping its allergenicity.We analyzed biochemical immunological interaction ligands with 1. We then investigated proteolytic by endosomal...
The cysteine protease enzyme legumain hydrolyzes peptide bonds with high specificity after asparagine and under more acidic conditions aspartic acid [Baker, E. N. J. Mol. Biol. 1980, 141, 441−484; Baker, N.; 1977, 111, 207–210; Drenth, J.; Biochemistry 1976, 15, 3731–3738; Menard, R.; Cell. Biochem. 1994, 137; Polgar, L. Eur. 1978, 88, 513–521; Storer, A. C.; Methods Enzymol. 244, 486–500. Remarkably, additionally exhibits ligase activity that prevails at pH > 5.5. atomic reaction mechanisms...
The in-depth characterization of the recently identified house dust mite (HDM) major allergen Der p 23 requires production its recombinant counterpart because natural is poorly extractable from fecal pellets. This study aimed to provide a detailed physico-chemical (rDer 23) as well investigate IgE reactivity in cohort HDM-allergic patients Thailand.Purified rDer 23, secreted Pichia pastoris, was characterized by mass spectrometry and circular dichroism analyses for chitin-binding activity....
Abstract Background The house dust mite (HDM ) allergen Der p 13 could be a lipid‐binding protein able to activate key innate signaling pathways in the initiation of allergic response. We investigated IgE reactivity recombinant (rDer 13), its activities, and capacity stimulate airway epithelium cells. Methods Purified rDer was characterized by mass spectrometry, circular dichroism, fluorescence‐based assays, silico structural prediction. IgE‐binding activity allergenic potential were...
Here, we develop PEPSeek, a web-server based software to allow higher performance in the identification of pathogen-derived epitope candidates detected via mass spectrometry MHC class I immunopeptidomes. We apply it human and mouse cell lines infected with either SARS-CoV-2, Listeria monocytogenes or Chlamydia trachomatis, thereby identifying large number novel antigens epitopes that prove be recognized by CD8+ T cells. In cells, identified antigenic peptide features suggested how processing...
The vacuolar cysteine protease legumain plays important functions in seed maturation and plant programmed cell death. Because of their dual ligase activity, legumains have become particular biotechnological interest, e.g. for the synthesis cyclic peptides drug design or protein engineering. However, molecular mechanisms behind activities are still poorly understood, limiting applications. Here, we present crystal structure Arabidopsis thaliana isoform β (AtLEGβ) its zymogen state. Combining...
Art v 1, Amb a 4, and Par h 1 are allergenic defensin-polyproline-linked proteins present in mugwort, ragweed, feverfew pollen, respectively. We aimed to investigate the physicochemical immunological features underlying different capacities of those allergens.Recombinant were expressed E. coli physicochemically characterized detail regarding identity, secondary structure, aggregation status. Allergenic activity was assessed by mediator releases assay, serum IgE reactivity, inhibition ELISA...
Bottom-up mass spectrometry-based proteomics utilizes proteolytic enzymes with well characterized specificities to generate peptides amenable for identification by high-throughput tandem spectrometry. Trypsin, which cuts specifically after the basic residues lysine and arginine, is predominant enzyme used proteome digestion, although proteases alternative are required detect sequences that not accessible tryptic digest. Here, we show human cysteine protease legumain exhibits a strict...
Actinoporins are small 18.5 kDa pore-forming toxins. A family of six actinoporin genes has been identified in the genome Hydra magnipapillata, and HALT-1 (Hydra actinoporin-like toxin-1) shown to have haemolytic activity. In this study, we used site-directed mutagenesis investigate role amino acids N-terminal region conserved aromatic cluster required for cell membrane binding. total 10 mutants were constructed tested their cytolytic activity on human erythrocytes HeLa cells, respectively....
Abstract SARS-CoV-2 infection causes severe symptoms in a subset of patients, suggesting the presence certain unknown risk factors. Although antibodies against receptor-binding domain (RBD) spike have been shown prevent infection, effects other protein domains are largely unknown. Here, we screened series anti-spike monoclonal from COVID-19 and found that some N-terminal (NTD) dramatically enhanced binding capacity to ACE2, thus increased SARS-CoV2 infectivity. Surprisingly, mutational...
Introduction: Understanding, which factors determine the immunogenicity and immune polarizing properties of proteins, is an important prerequisite for designing better vaccines immunotherapeutics. While extrinsic modulatory such as pathogen associated molecular patterns are well understood, far less known about contribution protein inherent features. Protein fold-stability represents intrinsic feature contributing to polarization by influencing amount peptide-MHC II complexes (pMHCII). Here,...
Knowledge of the susceptibility proteins to endolysosomal proteases provides valuable information on immunogenicity. Though Ole e 1-like are considered relevant allergens, little is known about their immunogenic properties and T cell epitopes. Thus, six representative molecules, i.e., 1, Fra Sal k 5, Che a Phl p 11 Pla l were investigated. Endolysosomal degradation peptide generation simulated using microsomal fractions JAWS II dendritic cells. Kinetics patterns evaluated by gel...
The discovery of many noncanonical peptides detectable with sensitive mass spectrometry inside, outside, and on cells shepherded the development novel methods for their identification, often not supported by a systematic benchmarking other methods. We here propose iBench, bioinformatic tool that can construct ground truth proteomics datasets cognate databases, thereby generating training court wherein methods, search engines, strategies be tested, performances estimated same tool. iBench...
Birch pollen allergy affects more than 20% of the European allergic population. On a molecular level, birch can be linked to two dominant allergens Bet v 1 and 2. 2 belongs profilin family, which is abundant in plant kingdom. Importantly, homologous profilins have conserved cysteine motif with currently unknown functional relevance. In particular, it whether relevant for disulfide formation what extent would affect profilins’ structural, immunological properties. Here we present crystal...